Olive leaves have an antioxidant capacity, and olive leaf extract can protect the blood, spleen and hippocampus in lead-poisoned mice. However, little is known about the effects of olive leaf extract on lead-induced b...Olive leaves have an antioxidant capacity, and olive leaf extract can protect the blood, spleen and hippocampus in lead-poisoned mice. However, little is known about the effects of olive leaf extract on lead-induced brain injury. This study was designed to determine whether olive leaf extract can inhibit lead-induced brain injury, and whether this effect is associated with antioxidant capacity. First, we established a mouse model of lead poisoning by continuous intragastric administration of lead acetate for 30 days. Two hours after successful model establishment, lead-poisoned mice were given olive leaf extract at doses of 250, 500 or 1 000 mg/kg daily by intragastric administration for 50 days. Under the transmission electron microscope, olive leaf extract attenuated neuronal and capillary injury and reduced damage to organelles and the matrix around the capillaries in the frontal lobe of the cerebral cortex in the lead-poisoned mice. Olive leaf extract at a dose of 1 000 mg/kg had the greatest protective effect. Spectrophotometry showed that olive leaf extract significantly in- creased the activities of superoxide dismutase, catalase, alkaline phosphatase and acid phes- phatase, while it reduced malondialdehyde content, in a dose-dependent manner. Furthermore, immunohistochemical staining revealed that olive leaf extract dose-dependently decreased Bax protein expression in the cerebral cortex of lead-poisoned mice. Our findings indicate that olive leaf extract can inhibit lead-induced brain injury by increasing antioxidant capacity and reducing apop- tosis.展开更多
Seeds of Indian mustard （Brassicajuncea （L.） Czern. et Coss.） were exposed to 0, 50, 100 and 150 mmol/L NaCI for 8 h and seeds were sown in an earthen pot. These stressed seedlings were subsequently sprayed with 1...Seeds of Indian mustard （Brassicajuncea （L.） Czern. et Coss.） were exposed to 0, 50, 100 and 150 mmol/L NaCI for 8 h and seeds were sown in an earthen pot. These stressed seedlings were subsequently sprayed with 10μmol/L salicylic acid （SA） at 30 d and were sampled at 60 d to assess the changes in growth, photosynthesis and antioxidant enzymes. The seedlings raised from the seeds treated with NaCI had significantly reduced growth and the activities of carbonic anhydrase, nitrate reductase and photosynthesis, and the decrease was proportional to the increase in NaCI concentration. However, the antioxidant enzymes （catalase, peroxidase and superoxide dismutase） and proline content was enhanced in response to NaCI and/or SA treatment, where their interaction had an additive effect. Moreover, the toxic effects generated by the lower concentration of NaCI （50 mmol/L） were completely overcome by the application of SA. It was, therefore, concluded that SA ameliorated the stress generated by NaCl through the alleviated antioxidant system.展开更多
Hydrogen can relieve tissue-damaging oxidative stress, inflammation and apoptosis. Injection of hydrogen-rich saline is an effective method for transporting molecular hydrogen. We hypothesized that hydrogen-rich salin...Hydrogen can relieve tissue-damaging oxidative stress, inflammation and apoptosis. Injection of hydrogen-rich saline is an effective method for transporting molecular hydrogen. We hypothesized that hydrogen-rich saline would promote the repair of spinal cord injury induced by Allen＇s method in rats. At 0.5, 1, 2, 4, 8, 12 and 24 hours after injury, then once daily for 2 weeks, 0.25 mL/kg hydrogen-rich saline was infused into the subarachnoid space through a catheter. Results at 24 hours, 48 hours, 1 week and 2 weeks after injury showed that hydrogen-rich saline markedly reduced cell death, inflammatory cell infiltration, serum malondialdehyde content, and caspa se-3 immunoreactivity, elevated serum superoxide dismutase activity and calcitonin gene-related peptide immunoreactivity, and improved motor function in the hindlimb. The present study confirms that hydrogen-rich saline injected within 2 weeks of injury effectively contributes to the repair of spinal cord injury in the acute stage.展开更多
基金supported by the Natural Science Foundation of Gansu Province,No.1107RJZK243a grant from Gansu Provincial Education Committee,No.1128B-01
文摘Olive leaves have an antioxidant capacity, and olive leaf extract can protect the blood, spleen and hippocampus in lead-poisoned mice. However, little is known about the effects of olive leaf extract on lead-induced brain injury. This study was designed to determine whether olive leaf extract can inhibit lead-induced brain injury, and whether this effect is associated with antioxidant capacity. First, we established a mouse model of lead poisoning by continuous intragastric administration of lead acetate for 30 days. Two hours after successful model establishment, lead-poisoned mice were given olive leaf extract at doses of 250, 500 or 1 000 mg/kg daily by intragastric administration for 50 days. Under the transmission electron microscope, olive leaf extract attenuated neuronal and capillary injury and reduced damage to organelles and the matrix around the capillaries in the frontal lobe of the cerebral cortex in the lead-poisoned mice. Olive leaf extract at a dose of 1 000 mg/kg had the greatest protective effect. Spectrophotometry showed that olive leaf extract significantly in- creased the activities of superoxide dismutase, catalase, alkaline phosphatase and acid phes- phatase, while it reduced malondialdehyde content, in a dose-dependent manner. Furthermore, immunohistochemical staining revealed that olive leaf extract dose-dependently decreased Bax protein expression in the cerebral cortex of lead-poisoned mice. Our findings indicate that olive leaf extract can inhibit lead-induced brain injury by increasing antioxidant capacity and reducing apop- tosis.
文摘Seeds of Indian mustard （Brassicajuncea （L.） Czern. et Coss.） were exposed to 0, 50, 100 and 150 mmol/L NaCI for 8 h and seeds were sown in an earthen pot. These stressed seedlings were subsequently sprayed with 10μmol/L salicylic acid （SA） at 30 d and were sampled at 60 d to assess the changes in growth, photosynthesis and antioxidant enzymes. The seedlings raised from the seeds treated with NaCI had significantly reduced growth and the activities of carbonic anhydrase, nitrate reductase and photosynthesis, and the decrease was proportional to the increase in NaCI concentration. However, the antioxidant enzymes （catalase, peroxidase and superoxide dismutase） and proline content was enhanced in response to NaCI and/or SA treatment, where their interaction had an additive effect. Moreover, the toxic effects generated by the lower concentration of NaCI （50 mmol/L） were completely overcome by the application of SA. It was, therefore, concluded that SA ameliorated the stress generated by NaCl through the alleviated antioxidant system.
基金supported by a grant from Hunan Provincial Science and Technology Ministry of China,No.2015JJ6116
文摘Hydrogen can relieve tissue-damaging oxidative stress, inflammation and apoptosis. Injection of hydrogen-rich saline is an effective method for transporting molecular hydrogen. We hypothesized that hydrogen-rich saline would promote the repair of spinal cord injury induced by Allen＇s method in rats. At 0.5, 1, 2, 4, 8, 12 and 24 hours after injury, then once daily for 2 weeks, 0.25 mL/kg hydrogen-rich saline was infused into the subarachnoid space through a catheter. Results at 24 hours, 48 hours, 1 week and 2 weeks after injury showed that hydrogen-rich saline markedly reduced cell death, inflammatory cell infiltration, serum malondialdehyde content, and caspa se-3 immunoreactivity, elevated serum superoxide dismutase activity and calcitonin gene-related peptide immunoreactivity, and improved motor function in the hindlimb. The present study confirms that hydrogen-rich saline injected within 2 weeks of injury effectively contributes to the repair of spinal cord injury in the acute stage.
