In previous work it was shown that mutation of site 1 in the downstream enhancer sequence (DE) led to ablation of enhancement. Mutation of the Wilms tumour factor element (Wt), situated in the Amh promoter between the...In previous work it was shown that mutation of site 1 in the downstream enhancer sequence (DE) led to ablation of enhancement. Mutation of the Wilms tumour factor element (Wt), situated in the Amh promoter between the tata box and the start of translation (TSS), also led to ablation of enhancement. This suggested that these sites may be the anchor points for a specific duplex factor bridging remote DNA elements to the promoter. Mutation analysis of the DNA sequence between sections 1 and 2 of DE was carried out by site directed mutagenesis. It is reported here that site 4 lying between DE1 and DE2, plays a key role in controlling the level of enhancement.展开更多
目的:比较不同配伍活血化瘀方药对肥胖大鼠皮下和内脏脂肪内脂素、脂联素等mRAN表达的影响,探讨化痰活血方的作用机制。方法:利用高脂饲料复制营养性肥胖大鼠模型,以活血方(血府逐瘀汤)、补气活血方(补阳还五汤)、化痰活血方(血府逐瘀...目的:比较不同配伍活血化瘀方药对肥胖大鼠皮下和内脏脂肪内脂素、脂联素等mRAN表达的影响,探讨化痰活血方的作用机制。方法:利用高脂饲料复制营养性肥胖大鼠模型,以活血方(血府逐瘀汤)、补气活血方(补阳还五汤)、化痰活血方(血府逐瘀汤合二陈汤)干预,利用实时荧光定量PCR技术检测皮下脂肪组织、内脏脂肪组织内脂素、脂联素等mRAN表达,酶联免疫法检测其血清浓度。使用SAS 9.13 for Windows系统进行统计分析。结果:活血方药可下调模型大鼠内脏脂肪、皮下脂肪的内脂素mRNA表达;补气活血方、化痰活血方对内脏脂肪的作用优于活血方;但各活血方对皮下脂肪内脂素mRNA表达的影响未见显著差异。活血化瘀方药可上调模型大鼠内脏脂肪的脂联素mRNA表达,但各活血方之间未见显著差异;补气活血方能够上调皮下脂肪的脂联素mRNA表达,但活血方、化痰活血方的作用未见显著差异。结论:内脏脂肪、皮下脂肪组织分泌功能存在显著差异,补气、活血、化痰药在肥胖病治疗中可能存在协同作用;气虚、痰湿与血瘀在肥胖病的发生发展过程中均具有重要意义。展开更多
[Objective] The paper was to study the expression characteristics and borer resistance of Bt gene of Songjing 9 （1C^＊）. [Method] With super japonica rice Songjing 9 as the receptor, transgenic crylC^＊ super japon...[Objective] The paper was to study the expression characteristics and borer resistance of Bt gene of Songjing 9 （1C^＊）. [Method] With super japonica rice Songjing 9 as the receptor, transgenic crylC^＊ super japonica rice Songjing 9 （1C^＊） regulated by ubi promoter was created by agrobacterium-mediated genetic transformation method. Ten strains of transgenic super japonica rice formed from different transformation events were planted in the field; the Tt-mRNA of leaf, stem-sheath and young spikelet at booting stage were detected by real-time fluorescent quantitative PCR; the Bt protein content of leaf, stem-sheath and young spikelet and harvested brown rice were detected by enzyme-linked immunosorbent assay （ELISA）; the white head rate damaged by Chilo suppressalis was manually investigated. [Result] The Bt-mRNA and Bt protein content of various organs and Bt protein content of mature brown rice at booting stage of Songjing 9 （1C^＊） significantly differed among strains; there was no correlation relationship in Bt-mRNA and Bt protein content between leaf and stem-sheath, between leaf and young spikelet, and between stem-sheath and young spikelet; there was no relation between Bt-mRNA or Bt protein content of leaf, stem-sheath and young spikelet and Bt protein content of brown rice; there was positive correlation between Bt-mRNA and Bt protein content in the same organ such as leaf, stem-sheath and young spikelet at booting stage; such strains as Songjing 9 （1C^＊）, whether the Bt gene expression was high or low, showed good resistance against C.suppressalis. This indicated that there were differences in Bt gene expression in different strains of transgenic cry1C^＊ super japonica rice or different organs of the same strain; there was no relation in Bt gene expression among different organs; the Bt-mRNA and protein content of the same organ usually had consistent expression; the Bt gene expression of various organs had nothing to do with the Bt protein content of brown rice展开更多
文摘In previous work it was shown that mutation of site 1 in the downstream enhancer sequence (DE) led to ablation of enhancement. Mutation of the Wilms tumour factor element (Wt), situated in the Amh promoter between the tata box and the start of translation (TSS), also led to ablation of enhancement. This suggested that these sites may be the anchor points for a specific duplex factor bridging remote DNA elements to the promoter. Mutation analysis of the DNA sequence between sections 1 and 2 of DE was carried out by site directed mutagenesis. It is reported here that site 4 lying between DE1 and DE2, plays a key role in controlling the level of enhancement.
文摘目的:比较不同配伍活血化瘀方药对肥胖大鼠皮下和内脏脂肪内脂素、脂联素等mRAN表达的影响,探讨化痰活血方的作用机制。方法:利用高脂饲料复制营养性肥胖大鼠模型,以活血方(血府逐瘀汤)、补气活血方(补阳还五汤)、化痰活血方(血府逐瘀汤合二陈汤)干预,利用实时荧光定量PCR技术检测皮下脂肪组织、内脏脂肪组织内脂素、脂联素等mRAN表达,酶联免疫法检测其血清浓度。使用SAS 9.13 for Windows系统进行统计分析。结果:活血方药可下调模型大鼠内脏脂肪、皮下脂肪的内脂素mRNA表达;补气活血方、化痰活血方对内脏脂肪的作用优于活血方;但各活血方对皮下脂肪内脂素mRNA表达的影响未见显著差异。活血化瘀方药可上调模型大鼠内脏脂肪的脂联素mRNA表达,但各活血方之间未见显著差异;补气活血方能够上调皮下脂肪的脂联素mRNA表达,但活血方、化痰活血方的作用未见显著差异。结论:内脏脂肪、皮下脂肪组织分泌功能存在显著差异,补气、活血、化痰药在肥胖病治疗中可能存在协同作用;气虚、痰湿与血瘀在肥胖病的发生发展过程中均具有重要意义。
基金Supported by National High Technology Research and Development Program(863 plan)of China(2014AA10A600)National Major Project for Transgenic Organism Breeding of China(2016ZX08001001-001-007)Open Project of Key Laboratory of Molecular Biology in Colleges and Universities of Heilongjiang Province(1206)
文摘[Objective] The paper was to study the expression characteristics and borer resistance of Bt gene of Songjing 9 （1C^＊）. [Method] With super japonica rice Songjing 9 as the receptor, transgenic crylC^＊ super japonica rice Songjing 9 （1C^＊） regulated by ubi promoter was created by agrobacterium-mediated genetic transformation method. Ten strains of transgenic super japonica rice formed from different transformation events were planted in the field; the Tt-mRNA of leaf, stem-sheath and young spikelet at booting stage were detected by real-time fluorescent quantitative PCR; the Bt protein content of leaf, stem-sheath and young spikelet and harvested brown rice were detected by enzyme-linked immunosorbent assay （ELISA）; the white head rate damaged by Chilo suppressalis was manually investigated. [Result] The Bt-mRNA and Bt protein content of various organs and Bt protein content of mature brown rice at booting stage of Songjing 9 （1C^＊） significantly differed among strains; there was no correlation relationship in Bt-mRNA and Bt protein content between leaf and stem-sheath, between leaf and young spikelet, and between stem-sheath and young spikelet; there was no relation between Bt-mRNA or Bt protein content of leaf, stem-sheath and young spikelet and Bt protein content of brown rice; there was positive correlation between Bt-mRNA and Bt protein content in the same organ such as leaf, stem-sheath and young spikelet at booting stage; such strains as Songjing 9 （1C^＊）, whether the Bt gene expression was high or low, showed good resistance against C.suppressalis. This indicated that there were differences in Bt gene expression in different strains of transgenic cry1C^＊ super japonica rice or different organs of the same strain; there was no relation in Bt gene expression among different organs; the Bt-mRNA and protein content of the same organ usually had consistent expression; the Bt gene expression of various organs had nothing to do with the Bt protein content of brown rice
