[Objectives]This study was conducted to clarify the species,occurrence and distribution characteristics of sugarcane rust pathogens in low-latitude plateau sugarcane rust areas of Yunnan Province and the phylogenetic ...[Objectives]This study was conducted to clarify the species,occurrence and distribution characteristics of sugarcane rust pathogens in low-latitude plateau sugarcane rust areas of Yunnan Province and the phylogenetic relationship between the pathogens and that between them and other rust fungi of the genus Puccinia.[Methods]Fifty seven sugarcane rust samples collected from the sugarcane areas of Baoshan,Lincang,Menghai,Menglian,Ximeng,Lancang and Wenshan in Yunnan were subjected to molecular identification.[Results]The four sugarcane rust samples from Haiyin1 hao in Menghai,Yunnan Province belonged to orange rust of sugarcane,and the pathogen was Puccinia kuehnii Butler.;and other 53 samples of sugarcane rust belonged to brown rust of sugarcane,and the pathogen was Puccinia melanocephala Syd.et P.Syd.The rDNA sequences of other rust fungi from the genus Puccinia were downloaded from GenBank and used for the construction of an NJ tree for systematic evolution analysis together with the sequences obtained in this study.The results showed that the four P.kuehnii sequences obtained in this study were clustered with Puccinia polysora(GenBank accession number:GU058024)into one group,and they were close genetically;the 53 P.melanocephala sequences were clustered with P.nakanishikii(GenBank accession number:GU058002),P.rufipes(GenBank accession number:AJ296545),Aecidium deutziae(GenBank accession number:KU309317)and P.coronata(GenBank accession number:DQ354526)into one group,and they had a close genetic relationship;and the genetic relationship of the two species of sugarcane rust fungi identified in this study was relatively distant.[Conclusions]This study provides a scientific basis for sugarcane rust epidemic prediction,disease resistance breeding,and precise prevention and control.展开更多
The sugarcane rust was the main disease causing damage on sugarcane leaves. The biological characteristics of the sugarcane rust pathogens found in Beihai were studied. The research results showed that the germination...The sugarcane rust was the main disease causing damage on sugarcane leaves. The biological characteristics of the sugarcane rust pathogens found in Beihai were studied. The research results showed that the germination of uredospores would be promoted under the dark condition or with the glucose concentration of 1.5%. The suitable temperature range for the germination of uredospore was 20 -25 ℃, 25 ℃3 was the optimum temperature. The range of relative humidity （RH） for the germination of uredospore was over 80%, the optimum relative humidity for the germination of uredospore was 100% ＋water drops with the germination rate of 28.3%. Urediospore could germinate in the pH value range of 4.1 - 11.1, the most suitable pH value was 7.0. At routine conditions, the longest survival period of uredospore was 120 days. Selection of resistant varieties and cultivation management was the key of control measures.展开更多
基金Supported by China Agriculture Research System of MOF and MARA(CARS-170303)Joint Special Project of Basic Agricultural Research in Yunnan Province(2018FG001-029)+1 种基金"Yunling Industrial Technology Leading Talents"Training Project(2018LJRC56)Special Fund for the Construction of Modern Agricultural Industry Technology System in Yunnan Province(YNGZTX-4-92)。
文摘[Objectives]This study was conducted to clarify the species,occurrence and distribution characteristics of sugarcane rust pathogens in low-latitude plateau sugarcane rust areas of Yunnan Province and the phylogenetic relationship between the pathogens and that between them and other rust fungi of the genus Puccinia.[Methods]Fifty seven sugarcane rust samples collected from the sugarcane areas of Baoshan,Lincang,Menghai,Menglian,Ximeng,Lancang and Wenshan in Yunnan were subjected to molecular identification.[Results]The four sugarcane rust samples from Haiyin1 hao in Menghai,Yunnan Province belonged to orange rust of sugarcane,and the pathogen was Puccinia kuehnii Butler.;and other 53 samples of sugarcane rust belonged to brown rust of sugarcane,and the pathogen was Puccinia melanocephala Syd.et P.Syd.The rDNA sequences of other rust fungi from the genus Puccinia were downloaded from GenBank and used for the construction of an NJ tree for systematic evolution analysis together with the sequences obtained in this study.The results showed that the four P.kuehnii sequences obtained in this study were clustered with Puccinia polysora(GenBank accession number:GU058024)into one group,and they were close genetically;the 53 P.melanocephala sequences were clustered with P.nakanishikii(GenBank accession number:GU058002),P.rufipes(GenBank accession number:AJ296545),Aecidium deutziae(GenBank accession number:KU309317)and P.coronata(GenBank accession number:DQ354526)into one group,and they had a close genetic relationship;and the genetic relationship of the two species of sugarcane rust fungi identified in this study was relatively distant.[Conclusions]This study provides a scientific basis for sugarcane rust epidemic prediction,disease resistance breeding,and precise prevention and control.
基金Supported by Special Fund Project for Basic Research in Guangxi Sugarcane Research Institute(G2008010)Special Fund of Modern Industrial Technology System Construction(NYCYTXGXCXTD-02)~~
文摘The sugarcane rust was the main disease causing damage on sugarcane leaves. The biological characteristics of the sugarcane rust pathogens found in Beihai were studied. The research results showed that the germination of uredospores would be promoted under the dark condition or with the glucose concentration of 1.5%. The suitable temperature range for the germination of uredospore was 20 -25 ℃, 25 ℃3 was the optimum temperature. The range of relative humidity （RH） for the germination of uredospore was over 80%, the optimum relative humidity for the germination of uredospore was 100% ＋water drops with the germination rate of 28.3%. Urediospore could germinate in the pH value range of 4.1 - 11.1, the most suitable pH value was 7.0. At routine conditions, the longest survival period of uredospore was 120 days. Selection of resistant varieties and cultivation management was the key of control measures.
