Thinopyrum elongatum （2n = 2x = 14, EE）, a wild relative of wheat, has been suggested as a potentially novel source of resistance to several major wheat diseases including Fusarium Head Blight （FHB）. In this study...Thinopyrum elongatum （2n = 2x = 14, EE）, a wild relative of wheat, has been suggested as a potentially novel source of resistance to several major wheat diseases including Fusarium Head Blight （FHB）. In this study, a series of wheat （cv. Chinese Spring, CS） substitution and ditelosomic lines, including Th. elongatum additions, were assessed for Type II resistance to FHB. Results indicated that the lines containing chromosome 7E of Th. elongatum gave a high level of resistance to FHB, wherein the infection did not spread beyond the inoculated floret. Furthermore, it was determined that the novel resistance gene（s） of 7E was located on the short-ann （7ES） based on sharp difference in FHB resistance between the two 7E ditelosomic lines for each arm. On the other hand, Th. elongatum chromosomes 5E and 6E likely contain gene（s） for susceptibility to FHB because the disease spreads rapidly within the inoculated spikes of these lines. Genomic in situ hybridization （GISH） analysis revealed that the alien chromosomes in the addition and substitution lines were intact, and the lines did not contain discernible genomic aberrations. GISH and multicolor-GISH analyses were further performed on three trans- location lines that also showed high levels of resistance to FHB. Lines TA3499 and TA3695 were shown to contain one pair of wheat-Th. elongatum translocated chromosomes involving fragments of 7D plus a segment of the 7E, while line TA3493 was found to contain one pair of wheat-Th, elongatum translocated chromosomes involving the D- and A-genome chromosomes of wheat. Thus, this study has established that the short-arm of chromosome 7E of Th. elongatum harbors gene（s） highly resistant to the spreading of FHB, and chromatin of 7E introgressed into wheat chromosomes largely retained the resistance, implicating the feasibility of using these lines as novel material for breeding FHB-resistant wheat cultivars.展开更多
以中国春-Synthetic 6x小麦染色体代换系及其亲本为材料,设置对照(0 mmol/L Na Cl)和盐胁迫(150 mmol/L Na Cl)2个处理,通过测定不同盐处理条件下代换系幼苗渗透调节物质Na+、K+、可溶性糖、可溶性蛋白及脯氨酸含量变化,探讨盐胁迫对小...以中国春-Synthetic 6x小麦染色体代换系及其亲本为材料,设置对照(0 mmol/L Na Cl)和盐胁迫(150 mmol/L Na Cl)2个处理,通过测定不同盐处理条件下代换系幼苗渗透调节物质Na+、K+、可溶性糖、可溶性蛋白及脯氨酸含量变化,探讨盐胁迫对小麦代换系幼苗渗透调节物质的影响,并对其调控相关特性的基因进行染色体定位。结果表明,在盐胁迫条件下,小麦代换系的无机渗透调节物质Na+、K+含量明显升高,有机渗透调节物质可溶性糖、可溶性蛋白及脯氨酸含量显著增加。对相关性状的染色体定位分析表明,Synthetic 6x的1A、2A、3B、7B、1D、4D和7D染色体上存在抑制Na+含量升高的基因,4A、7A染色体上可能存在使K+含量升高的基因;6A、7A和7D染色体上可能存在使可溶性糖含量升高的基因,1A、2A、4A和6A染色体上可能存在使可溶性蛋白含量升高的基因,7A、6D染色体上可能存在使脯氨酸含量升高的基因。即Synthetic 6x的第4、7染色体(4A、4D;7A、7D)上可能含有调控无机调节物质的基因,第6染色体上(6A、6D)可能含有调控有机渗透调节物质的基因。展开更多
A set of T. aestivum-L. elongatum substitution lines were studied on yellow rust resistance at seedling stage, inheritance of the resistance and esterase-5 (Est-5) analysis. The results demonstrated that L. elongatum ...A set of T. aestivum-L. elongatum substitution lines were studied on yellow rust resistance at seedling stage, inheritance of the resistance and esterase-5 (Est-5) analysis. The results demonstrated that L. elongatum carried a new yellow rust resistance gene(s), which was dominant and located on chromosome 3E of L. elongatum. The biochemical locus encoding Est-5 was also located on chromosome 3E, and was tentatively named Est-E5, which was co-segregant with the Yr gene(s) in wheat background. In addition, the transmission frequencies of chromosome 3E in 3A/3E and 3D/3E hybrids selfing were significantly higher than that of chromosome 3E in 3B/3E hybrid, which was probably due to the difference on genetic relationships among A, B, D and E genomes.展开更多
基金supported by the grant of the National High Technology Research and Development Program("863"Program)of China(No.2011AA100101)
文摘Thinopyrum elongatum （2n = 2x = 14, EE）, a wild relative of wheat, has been suggested as a potentially novel source of resistance to several major wheat diseases including Fusarium Head Blight （FHB）. In this study, a series of wheat （cv. Chinese Spring, CS） substitution and ditelosomic lines, including Th. elongatum additions, were assessed for Type II resistance to FHB. Results indicated that the lines containing chromosome 7E of Th. elongatum gave a high level of resistance to FHB, wherein the infection did not spread beyond the inoculated floret. Furthermore, it was determined that the novel resistance gene（s） of 7E was located on the short-ann （7ES） based on sharp difference in FHB resistance between the two 7E ditelosomic lines for each arm. On the other hand, Th. elongatum chromosomes 5E and 6E likely contain gene（s） for susceptibility to FHB because the disease spreads rapidly within the inoculated spikes of these lines. Genomic in situ hybridization （GISH） analysis revealed that the alien chromosomes in the addition and substitution lines were intact, and the lines did not contain discernible genomic aberrations. GISH and multicolor-GISH analyses were further performed on three trans- location lines that also showed high levels of resistance to FHB. Lines TA3499 and TA3695 were shown to contain one pair of wheat-Th. elongatum translocated chromosomes involving fragments of 7D plus a segment of the 7E, while line TA3493 was found to contain one pair of wheat-Th, elongatum translocated chromosomes involving the D- and A-genome chromosomes of wheat. Thus, this study has established that the short-arm of chromosome 7E of Th. elongatum harbors gene（s） highly resistant to the spreading of FHB, and chromatin of 7E introgressed into wheat chromosomes largely retained the resistance, implicating the feasibility of using these lines as novel material for breeding FHB-resistant wheat cultivars.
文摘以中国春-Synthetic 6x小麦染色体代换系及其亲本为材料,设置对照(0 mmol/L Na Cl)和盐胁迫(150 mmol/L Na Cl)2个处理,通过测定不同盐处理条件下代换系幼苗渗透调节物质Na+、K+、可溶性糖、可溶性蛋白及脯氨酸含量变化,探讨盐胁迫对小麦代换系幼苗渗透调节物质的影响,并对其调控相关特性的基因进行染色体定位。结果表明,在盐胁迫条件下,小麦代换系的无机渗透调节物质Na+、K+含量明显升高,有机渗透调节物质可溶性糖、可溶性蛋白及脯氨酸含量显著增加。对相关性状的染色体定位分析表明,Synthetic 6x的1A、2A、3B、7B、1D、4D和7D染色体上存在抑制Na+含量升高的基因,4A、7A染色体上可能存在使K+含量升高的基因;6A、7A和7D染色体上可能存在使可溶性糖含量升高的基因,1A、2A、4A和6A染色体上可能存在使可溶性蛋白含量升高的基因,7A、6D染色体上可能存在使脯氨酸含量升高的基因。即Synthetic 6x的第4、7染色体(4A、4D;7A、7D)上可能含有调控无机调节物质的基因,第6染色体上(6A、6D)可能含有调控有机渗透调节物质的基因。
文摘A set of T. aestivum-L. elongatum substitution lines were studied on yellow rust resistance at seedling stage, inheritance of the resistance and esterase-5 (Est-5) analysis. The results demonstrated that L. elongatum carried a new yellow rust resistance gene(s), which was dominant and located on chromosome 3E of L. elongatum. The biochemical locus encoding Est-5 was also located on chromosome 3E, and was tentatively named Est-E5, which was co-segregant with the Yr gene(s) in wheat background. In addition, the transmission frequencies of chromosome 3E in 3A/3E and 3D/3E hybrids selfing were significantly higher than that of chromosome 3E in 3B/3E hybrid, which was probably due to the difference on genetic relationships among A, B, D and E genomes.
