Plants use sophisticated mechanisms of gene expression to control senescence in response to environmental stress or aging.ORE1(Arabidopsis thaliana NAC092)is a master regulator of senescence that belongs to the plant-...Plants use sophisticated mechanisms of gene expression to control senescence in response to environmental stress or aging.ORE1(Arabidopsis thaliana NAC092)is a master regulator of senescence that belongs to the plant-specific NAC transcription factor protein family.ORE1 has been reported to bind to multiple DNA targets to orchestrate leaf senescence,yet the mechanistic basis for recognition of the cognate gene sequence remains unclear.Here,we report the crystal structure of the ORE1-NAC domain alone and its DNA-binding form.The structure of DNA-bound ORE1-NAC revealed the molecular basis for nucleobase recognition and phosphate backbone interactions.We showthat local versatility in the DNA-binding site,in combination with domain flexibility of the ORE-NAC homodimer,is crucial for the maintenance of binding to intrinsically flexible DNA.Our results provide a platformfor understanding other plant-specific NAC protein-DNA interactions as well as insight into the structural basis of NAC regulators in plants of agronomic and scientific importance.展开更多
The multistep phosphorelay （MSP） is a central signaling pathway in plants integrating a wide spectrum of hormonal and environmental inputs and controlling numerous developmental adaptations. For the thor- ough compr...The multistep phosphorelay （MSP） is a central signaling pathway in plants integrating a wide spectrum of hormonal and environmental inputs and controlling numerous developmental adaptations. For the thor- ough comprehension of the molecular mechanisms underlying the MSP-mediated signal recognition and transduction, the detailed structural characterization of individual members of the pathway is critical. In this review we describe and discuss the recently known crystal and nuclear magnetic resonance structures of proteins acting in MSP signaling in higher plants, focusing particularly on cytokinin and ethylene signaling in Arabidopsis thaliana. We discuss the range of functional aspects of available structural infor- mation including determination of ligand specificity, activation of the receptor via its autophosphorylaUon, and downstream signal transduction through the phosphorelay. We compare the plant structures with their bacterial counterparts and show that although the overall similarity is high, the differences in structural de- tails are frequent and functionally important. Finally, we discuss emerging knowledge on molecular recog- nition mechanisms in the MSP, and mention the latest findings regarding structural determinants of signaling specificity in the Arabidopsis MSP that could serve as a general model of this pathway in all higher plants.展开更多
The saturation problem is the one of the most common handicaps for applying to real applications, especially the actuator saturation. This paper focuses on the robustness of the sliding mode control （SMC） which inco...The saturation problem is the one of the most common handicaps for applying to real applications, especially the actuator saturation. This paper focuses on the robustness of the sliding mode control （SMC） which incorporates a saturation constraint technique compared to classical linear quadratic regulator （LQR） with saturation. In the first step, the authors present a design methodology of SMC of a class of linear saturated systems. The authors present the structure of the saturation, after that the synthesis of the sliding surface is formulate as a problem of root clustering, which leads to the development of a continuous and non-linear control law that ensures the reaching condition of the sliding mode. The second step is devoted to present briefly the LQR controller technique. Finally, to validate results, the authors demonstrate an example of a quarter of vehicle system.展开更多
基金supported by the Korean Ministry of Science,Information/Communication Technology and Future Planning(NRF-2021R1A4A1031754,NRF-2019M3E5D6066058,and NRF-2022R1F1A1068269)I.C.was funded by BK21 Plus(4120200313623)of the Ministry of Education,Korea.
文摘Plants use sophisticated mechanisms of gene expression to control senescence in response to environmental stress or aging.ORE1(Arabidopsis thaliana NAC092)is a master regulator of senescence that belongs to the plant-specific NAC transcription factor protein family.ORE1 has been reported to bind to multiple DNA targets to orchestrate leaf senescence,yet the mechanistic basis for recognition of the cognate gene sequence remains unclear.Here,we report the crystal structure of the ORE1-NAC domain alone and its DNA-binding form.The structure of DNA-bound ORE1-NAC revealed the molecular basis for nucleobase recognition and phosphate backbone interactions.We showthat local versatility in the DNA-binding site,in combination with domain flexibility of the ORE-NAC homodimer,is crucial for the maintenance of binding to intrinsically flexible DNA.Our results provide a platformfor understanding other plant-specific NAC protein-DNA interactions as well as insight into the structural basis of NAC regulators in plants of agronomic and scientific importance.
文摘The multistep phosphorelay （MSP） is a central signaling pathway in plants integrating a wide spectrum of hormonal and environmental inputs and controlling numerous developmental adaptations. For the thor- ough comprehension of the molecular mechanisms underlying the MSP-mediated signal recognition and transduction, the detailed structural characterization of individual members of the pathway is critical. In this review we describe and discuss the recently known crystal and nuclear magnetic resonance structures of proteins acting in MSP signaling in higher plants, focusing particularly on cytokinin and ethylene signaling in Arabidopsis thaliana. We discuss the range of functional aspects of available structural infor- mation including determination of ligand specificity, activation of the receptor via its autophosphorylaUon, and downstream signal transduction through the phosphorelay. We compare the plant structures with their bacterial counterparts and show that although the overall similarity is high, the differences in structural de- tails are frequent and functionally important. Finally, we discuss emerging knowledge on molecular recog- nition mechanisms in the MSP, and mention the latest findings regarding structural determinants of signaling specificity in the Arabidopsis MSP that could serve as a general model of this pathway in all higher plants.
文摘The saturation problem is the one of the most common handicaps for applying to real applications, especially the actuator saturation. This paper focuses on the robustness of the sliding mode control （SMC） which incorporates a saturation constraint technique compared to classical linear quadratic regulator （LQR） with saturation. In the first step, the authors present a design methodology of SMC of a class of linear saturated systems. The authors present the structure of the saturation, after that the synthesis of the sliding surface is formulate as a problem of root clustering, which leads to the development of a continuous and non-linear control law that ensures the reaching condition of the sliding mode. The second step is devoted to present briefly the LQR controller technique. Finally, to validate results, the authors demonstrate an example of a quarter of vehicle system.
