AIM To explore the relationship between the rules of Cx gene expression and cellular differentiation in organs of different embryonic stages. METHODS A series of Cx gene serving as molecular probes and the Northern...AIM To explore the relationship between the rules of Cx gene expression and cellular differentiation in organs of different embryonic stages. METHODS A series of Cx gene serving as molecular probes and the Northern blot hybrydization were employed to study the Cx gene expression. RESULTS Cx31, Cx31 1, Cx46 did not express while other Cx genes expressed in the embryonic liver and stomach. The Cx gene expression in the liver and stomach showed different state at different embryonic stages. The Cx gene expression had organic diversity. The expression of Cx26 gene was overlapping in the above organs. Cx43 did not express in the human liver after birth, but it expressed in the embryonic stage. CONCLUSION The expression state of Cx genes is concordant with cellular differentiation. It might be a key candidate gene to regulate some differentiational events associated with cellular differentiation, proliferation, and morphogenesis in the early embryo.展开更多
目的:探讨Id2(inhibitor of DNAbinding or inhibitor of differentiation)在SGC-7901和Colo-205细胞株中小亚群(sidepopulation,SP)细胞中的表达,并鉴定其是否为胃肠道癌干样细胞的特异性标志。方法:用Hoechest33342染色检测SGC-7901和...目的:探讨Id2(inhibitor of DNAbinding or inhibitor of differentiation)在SGC-7901和Colo-205细胞株中小亚群(sidepopulation,SP)细胞中的表达,并鉴定其是否为胃肠道癌干样细胞的特异性标志。方法:用Hoechest33342染色检测SGC-7901和Colo-205细胞株中SP细胞的比例;通过荧光免疫细胞化学染色比较Id2在SP细胞与非SP细胞中表达的差异。结果:胃癌SGC-7901、结肠癌Colo-205细胞中SP细胞的比例分别为3.8%和4.3%;Id2在SGC-7901细胞SP中的阳性表达率为34.2%,显著低于在非SP中的阳性表达率97.7%,P<0.001,而Id2在Colo-205细胞SP中的阳性表达率达77.4%,显著高于在非SP中的阳性表达率4.8%,P<0.001。结论:Id2可能是胃癌SGC-7901细胞中SP细胞的阴性分子标志,同时可能是结肠癌Colo-205细胞中SP细胞的阳性分子标志。展开更多
文摘AIM To explore the relationship between the rules of Cx gene expression and cellular differentiation in organs of different embryonic stages. METHODS A series of Cx gene serving as molecular probes and the Northern blot hybrydization were employed to study the Cx gene expression. RESULTS Cx31, Cx31 1, Cx46 did not express while other Cx genes expressed in the embryonic liver and stomach. The Cx gene expression in the liver and stomach showed different state at different embryonic stages. The Cx gene expression had organic diversity. The expression of Cx26 gene was overlapping in the above organs. Cx43 did not express in the human liver after birth, but it expressed in the embryonic stage. CONCLUSION The expression state of Cx genes is concordant with cellular differentiation. It might be a key candidate gene to regulate some differentiational events associated with cellular differentiation, proliferation, and morphogenesis in the early embryo.
文摘目的:探讨Id2(inhibitor of DNAbinding or inhibitor of differentiation)在SGC-7901和Colo-205细胞株中小亚群(sidepopulation,SP)细胞中的表达,并鉴定其是否为胃肠道癌干样细胞的特异性标志。方法:用Hoechest33342染色检测SGC-7901和Colo-205细胞株中SP细胞的比例;通过荧光免疫细胞化学染色比较Id2在SP细胞与非SP细胞中表达的差异。结果:胃癌SGC-7901、结肠癌Colo-205细胞中SP细胞的比例分别为3.8%和4.3%;Id2在SGC-7901细胞SP中的阳性表达率为34.2%,显著低于在非SP中的阳性表达率97.7%,P<0.001,而Id2在Colo-205细胞SP中的阳性表达率达77.4%,显著高于在非SP中的阳性表达率4.8%,P<0.001。结论:Id2可能是胃癌SGC-7901细胞中SP细胞的阴性分子标志,同时可能是结肠癌Colo-205细胞中SP细胞的阳性分子标志。
