The present study was designed to analyze the major constituents in Prunellae Spica and establish a method for simultaneous determination of two constituents contained in Prunellae Spica. High performance liquid chrom...The present study was designed to analyze the major constituents in Prunellae Spica and establish a method for simultaneous determination of two constituents contained in Prunellae Spica. High performance liquid chromatography coupled with time-of-flight mass spectrometry(HPLC-QTOF-MS/MS) technique was used to identify the constituents in the extractive of Prunellae Spica. High performance liquid chromatography coupled with evaporative light scattering detection(HPLC-ELSD) was used to simultaneously quantify two kinds of constituents contained in Prunellae Spica. Principal component analysis(PCA) was applied to compare the similarity and difference among samples from different regions of China. In the present study, 22 compounds were identified and some new fragmental pathways of triterpenic acids were discovered. An accurate and reliable HPLC-ELSD method was developed and validated for the first time to simultaneously quantify multiple constituents, including rosmarinic acid, maslinic acid, corosolic acid, betulin, oleanolic acid, and ursolic acid in the extract of Prunellae Spica.(PCA) revealed some similarities and differences among different samples from different regions of China. In conclusion, our results from this study would be helpful in establishing a scientific and rational quality control method for Prunellae Spica.展开更多
Background Most orthopedic surgeons prefer spica cast immobilization in children for 4 to 12 weeks after surgical hip reconstruction in children with developmental hip dysplasia.This challenging treatment may be assoc...Background Most orthopedic surgeons prefer spica cast immobilization in children for 4 to 12 weeks after surgical hip reconstruction in children with developmental hip dysplasia.This challenging treatment may be associated with complica-tions.Studies are lacking that focus on early mobilization without casting for postoperative care after hip reconstruction.Methods Twenty-seven children(3.4±2.0 years),including 33 hips with developmental hip dysplasia(DDH)and dislocation of the hip(Tonnis grade 1 to 4),who underwent hip reconstruction(Dega acetabuloplasty,varisation-derotation osteotomy and facultative open reduction)were retrospectively included in this study.Postoperatively the patients were placed in an individual foam shell with 30 degrees of hip abduction,hip extension,and neutral rotation.Early mobilization physiotherapy was performed within the first few days after the surgery under epidural anaesthesia.Full weight bearing was allowed after 3-4 weeks.All children received a clinical examination and radiographic evaluation before and after surgical intervention.The follow-up period was 12.3±2.9 months.Results On average,the postoperative acetabular index decreased significantly from 36.9 to 21.7 degrees and the center-edge angle increased from 9.9 to 28.6 degrees.All hips had reached Tonnis grade 1 at the time of the last follow-up.No complications such as dislocation of the bone wedge,avascular necrosis of the acetabulum or femur,lack of non-union,or nerve injury,were reported.Conclusions In this cohort study,hip reconstruction was successful according to clinical and radiographic outcome param-eters after early mobilization without cast therapy.Early mobilization may be used as an alternative treatment option after hip reconstruction in DDH.展开更多
Introduction: Femoral shaft fractures are among the most common pediatric injury, which have the highest incidence among other pediatric fractures, and treatment of them carries a lot of controversies. Aim of the stud...Introduction: Femoral shaft fractures are among the most common pediatric injury, which have the highest incidence among other pediatric fractures, and treatment of them carries a lot of controversies. Aim of the study: To compare the outcome of fixation of femoral shaft fracture by elastic nail with Spica cast. Patient and method: This prospective comparative study was carried out in Sulaimani Teaching Hospital, in the period from the 10<sup>th</sup> of December 2011 to the 10<sup>th</sup> of June 2012, for two groups of children whose ages were between 4 - 12 years (average 6.61 years), sustained traumatic femoral shaft fractures with follow-up period of about 6 months. The first group was consisting of 30 children treated by Elastic Intramedullary Nail, while the second group was consisted of 30 children treated by hip Spica. The selection was made on random bases. Results: Age range was between 4 - 12 years and showed male predominance in both groups with ratio of about 2:1 while fracture site showed predominance of the midshaft pattern in both groups. This study showed highly significant deference (P value of 0.001) between the two groups (Spica group of 3 days versus Nail group median of rate 4.5 days) in the admission period. Our sample showed shortening of about 2 cm in 2 case in the Nail group (6.7%) versus 6 cases (20%) in Spica group. We reported 4 cases of wound infection (13.3%) and 4 cases of pin site irritation (13.3%) in nail group. A higher rate of malunion was observed in the Spica group (10 cases more than 10° angulation in coronal plane) while the Nail group reported 2 case more than 10° in coronal plane. The mean operative time for Nail group was 55 minutes while in Spica group was 30 minutes. We reported shorter time to start mobilization and walking with support or independently in the nail group (weight bearing time 7.2 weeks) compared with the Spica casting group (weight bearing time 7.5 weeks). Conclusion: Elastic nail fixation yields better outcome for femoral shaft fracture in form o展开更多
As important drugs for the treatment of nodular goiter(NG),Prunella Spica preparations are widely used clinically,and have a significant effect on NG.Various active ingredients in the preparations intervene in the for...As important drugs for the treatment of nodular goiter(NG),Prunella Spica preparations are widely used clinically,and have a significant effect on NG.Various active ingredients in the preparations intervene in the formation of NG by inhibiting the proliferation of thyroid follicular cells,promoting cell apoptosis,regulating immunity,improving the microcirculation of thyroid tissue and other mechanisms,and can reduce the postoperative recurrence of NG.展开更多
[Objectives] The study aimed to optimize the extraction process of Prunellae Spica compound to provide an experimental basis for the development and utilization of the compound. [Methods]Through orthogonal test,the op...[Objectives] The study aimed to optimize the extraction process of Prunellae Spica compound to provide an experimental basis for the development and utilization of the compound. [Methods]Through orthogonal test,the optimal extraction process of Prunellae Spica compound was chosen by using the yield of dry paste and ursolic acid content as evaluation indicators. [Results]The optimal extraction process of Prunellae Spica compound is as follows: solvent dosage was 16 times,and extraction frequency was 3 times,while extraction time was 1 h.[Conclusions]The extraction process of Prunellae Spica compound was stable and feasible,and this study can provide a theoretical basis for further preparation of the compound.展开更多
[Objectives]This study aimed to establish HPLC chromatograms of decoction pieces,standard decoction and formula granules of Spica Prunellae.[Methods]The chromatographic conditions were as follows:column,SHISEIDO CAPCE...[Objectives]This study aimed to establish HPLC chromatograms of decoction pieces,standard decoction and formula granules of Spica Prunellae.[Methods]The chromatographic conditions were as follows:column,SHISEIDO CAPCELL PAK C18 MGII column(4.6 mm×250 mm,5μm);mobile phase,acetonitrile-0.1%phosphoric acid;gradient elution;detection wavelength,280 nm;flow rate,1.0 mL/min;and column temperature,30℃.The correlation between the decoction pieces,standard decoction and formula granules of Spica Prunellae was analyzed by specific chromatograms.[Results]The fingerprint chromatograms of decoction pieces,standard decoction and formula granules of Spica Prunellae showed five common peaks,with good correlation.Among the five common peaks,four of them were tanshinol,protocatechuic acid,caffeic acid and rosmarinic acid.[Conclusions]The main chemical constituents of decoction pieces,standard decoction and formula granules of Spica Prunellae are basically the same.The HPLC specific chromatogram established can be used for the quality control of Spica Prunellae formula granules.展开更多
基金supported by National Natural Science Foundation of China(Nos.8127400481473317)+1 种基金2011’Program for Excellent Scientific and Technological Innovation Team of Jiangsu Higher Educationthe Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘The present study was designed to analyze the major constituents in Prunellae Spica and establish a method for simultaneous determination of two constituents contained in Prunellae Spica. High performance liquid chromatography coupled with time-of-flight mass spectrometry(HPLC-QTOF-MS/MS) technique was used to identify the constituents in the extractive of Prunellae Spica. High performance liquid chromatography coupled with evaporative light scattering detection(HPLC-ELSD) was used to simultaneously quantify two kinds of constituents contained in Prunellae Spica. Principal component analysis(PCA) was applied to compare the similarity and difference among samples from different regions of China. In the present study, 22 compounds were identified and some new fragmental pathways of triterpenic acids were discovered. An accurate and reliable HPLC-ELSD method was developed and validated for the first time to simultaneously quantify multiple constituents, including rosmarinic acid, maslinic acid, corosolic acid, betulin, oleanolic acid, and ursolic acid in the extract of Prunellae Spica.(PCA) revealed some similarities and differences among different samples from different regions of China. In conclusion, our results from this study would be helpful in establishing a scientific and rational quality control method for Prunellae Spica.
文摘Background Most orthopedic surgeons prefer spica cast immobilization in children for 4 to 12 weeks after surgical hip reconstruction in children with developmental hip dysplasia.This challenging treatment may be associated with complica-tions.Studies are lacking that focus on early mobilization without casting for postoperative care after hip reconstruction.Methods Twenty-seven children(3.4±2.0 years),including 33 hips with developmental hip dysplasia(DDH)and dislocation of the hip(Tonnis grade 1 to 4),who underwent hip reconstruction(Dega acetabuloplasty,varisation-derotation osteotomy and facultative open reduction)were retrospectively included in this study.Postoperatively the patients were placed in an individual foam shell with 30 degrees of hip abduction,hip extension,and neutral rotation.Early mobilization physiotherapy was performed within the first few days after the surgery under epidural anaesthesia.Full weight bearing was allowed after 3-4 weeks.All children received a clinical examination and radiographic evaluation before and after surgical intervention.The follow-up period was 12.3±2.9 months.Results On average,the postoperative acetabular index decreased significantly from 36.9 to 21.7 degrees and the center-edge angle increased from 9.9 to 28.6 degrees.All hips had reached Tonnis grade 1 at the time of the last follow-up.No complications such as dislocation of the bone wedge,avascular necrosis of the acetabulum or femur,lack of non-union,or nerve injury,were reported.Conclusions In this cohort study,hip reconstruction was successful according to clinical and radiographic outcome param-eters after early mobilization without cast therapy.Early mobilization may be used as an alternative treatment option after hip reconstruction in DDH.
文摘Introduction: Femoral shaft fractures are among the most common pediatric injury, which have the highest incidence among other pediatric fractures, and treatment of them carries a lot of controversies. Aim of the study: To compare the outcome of fixation of femoral shaft fracture by elastic nail with Spica cast. Patient and method: This prospective comparative study was carried out in Sulaimani Teaching Hospital, in the period from the 10<sup>th</sup> of December 2011 to the 10<sup>th</sup> of June 2012, for two groups of children whose ages were between 4 - 12 years (average 6.61 years), sustained traumatic femoral shaft fractures with follow-up period of about 6 months. The first group was consisting of 30 children treated by Elastic Intramedullary Nail, while the second group was consisted of 30 children treated by hip Spica. The selection was made on random bases. Results: Age range was between 4 - 12 years and showed male predominance in both groups with ratio of about 2:1 while fracture site showed predominance of the midshaft pattern in both groups. This study showed highly significant deference (P value of 0.001) between the two groups (Spica group of 3 days versus Nail group median of rate 4.5 days) in the admission period. Our sample showed shortening of about 2 cm in 2 case in the Nail group (6.7%) versus 6 cases (20%) in Spica group. We reported 4 cases of wound infection (13.3%) and 4 cases of pin site irritation (13.3%) in nail group. A higher rate of malunion was observed in the Spica group (10 cases more than 10° angulation in coronal plane) while the Nail group reported 2 case more than 10° in coronal plane. The mean operative time for Nail group was 55 minutes while in Spica group was 30 minutes. We reported shorter time to start mobilization and walking with support or independently in the nail group (weight bearing time 7.2 weeks) compared with the Spica casting group (weight bearing time 7.5 weeks). Conclusion: Elastic nail fixation yields better outcome for femoral shaft fracture in form o
基金Supported by the Key Project of Teaching Reform Research of Taishan Vocational College of Nursing(202307).
文摘As important drugs for the treatment of nodular goiter(NG),Prunella Spica preparations are widely used clinically,and have a significant effect on NG.Various active ingredients in the preparations intervene in the formation of NG by inhibiting the proliferation of thyroid follicular cells,promoting cell apoptosis,regulating immunity,improving the microcirculation of thyroid tissue and other mechanisms,and can reduce the postoperative recurrence of NG.
基金Supported by Talent Training and Construction Project of Professor Yang Shilin’s Team in 2017(YSL17010)
文摘[Objectives] The study aimed to optimize the extraction process of Prunellae Spica compound to provide an experimental basis for the development and utilization of the compound. [Methods]Through orthogonal test,the optimal extraction process of Prunellae Spica compound was chosen by using the yield of dry paste and ursolic acid content as evaluation indicators. [Results]The optimal extraction process of Prunellae Spica compound is as follows: solvent dosage was 16 times,and extraction frequency was 3 times,while extraction time was 1 h.[Conclusions]The extraction process of Prunellae Spica compound was stable and feasible,and this study can provide a theoretical basis for further preparation of the compound.
基金Supported by Scientific Research and Technology Development Project of Nanning City(20173158-5).
文摘[Objectives]This study aimed to establish HPLC chromatograms of decoction pieces,standard decoction and formula granules of Spica Prunellae.[Methods]The chromatographic conditions were as follows:column,SHISEIDO CAPCELL PAK C18 MGII column(4.6 mm×250 mm,5μm);mobile phase,acetonitrile-0.1%phosphoric acid;gradient elution;detection wavelength,280 nm;flow rate,1.0 mL/min;and column temperature,30℃.The correlation between the decoction pieces,standard decoction and formula granules of Spica Prunellae was analyzed by specific chromatograms.[Results]The fingerprint chromatograms of decoction pieces,standard decoction and formula granules of Spica Prunellae showed five common peaks,with good correlation.Among the five common peaks,four of them were tanshinol,protocatechuic acid,caffeic acid and rosmarinic acid.[Conclusions]The main chemical constituents of decoction pieces,standard decoction and formula granules of Spica Prunellae are basically the same.The HPLC specific chromatogram established can be used for the quality control of Spica Prunellae formula granules.
