SecA is the essential component of the signal-peptide dependent translocation pathway in Escherichia coil （E.coh）. The structure and function of SecA must be known to understand the molecular mechanism of preprotein...SecA is the essential component of the signal-peptide dependent translocation pathway in Escherichia coil （E.coh）. The structure and function of SecA must be known to understand the molecular mechanism of preprotein translocation. The high flexibility of SecA causes a dynamic conformational heterogeneity which presents a barrier to the growth of crystals of high diffraction quality. Electron microscopy was used to resolve the macromolecular structure of SecA in solution by negative staining and single particle analysis at a resolution of 2.9 nm. The structure of E. coil SecA is similar to the dimeric form of Bacillus subtilis SecA and is 10 nm × 10 nm × 5 nm in size.展开更多
A nuclear microprobe with high spatial resolution and high analyti- cal sensitivity was applied to analyze atmospheric aerosol at five monitoring sites in Shanghai city. Meantime, a new pattern recognition technique, ...A nuclear microprobe with high spatial resolution and high analyti- cal sensitivity was applied to analyze atmospheric aerosol at five monitoring sites in Shanghai city. Meantime, a new pattern recognition technique, which used the micro PIXE spectrum of a single aerosol particle as its fingerprint, was developed to identify the origin of the particle. The results showed that the major contributors to the at- mosphere pollution were soil dust (31.6%), building dust (30.8%), and the next were vehicle exhaust (13.7%), metallurgic industry excrements (5.6%), oil combustion (5%) and coal combustion (2.3%). Besides these, about 10% of the particles could not be identified. Based on the cluster analysis of these particles, they could be divided into eight groups. By inference, they might belong to some sub-pollution sources from soil dust, building dust and metallurgic industry excrements. Moreover, some new pollution sources from tyres and chemical plants were also revealed.展开更多
Scanning electron microscope energy dispersive X ray analysis system (SEM EDX) is able to provide analytical data for each single particle. Hundreds of particles are usually analyzed for an aerosol sample. Both physic...Scanning electron microscope energy dispersive X ray analysis system (SEM EDX) is able to provide analytical data for each single particle. Hundreds of particles are usually analyzed for an aerosol sample. Both physical and chemical characterization can be performed in terms of particle size and particle class compositional data, respectively. Mineral dusts such as aluminosilicates and quartz particles as well as sea salt particles featured coarse fractions. Fine fractions were dominated by various sulfur containing and carbonaceous particles.展开更多
基金Supported by the National Natural Science Foundation of China (Nos. 30170196 and 30330160)
文摘SecA is the essential component of the signal-peptide dependent translocation pathway in Escherichia coil （E.coh）. The structure and function of SecA must be known to understand the molecular mechanism of preprotein translocation. The high flexibility of SecA causes a dynamic conformational heterogeneity which presents a barrier to the growth of crystals of high diffraction quality. Electron microscopy was used to resolve the macromolecular structure of SecA in solution by negative staining and single particle analysis at a resolution of 2.9 nm. The structure of E. coil SecA is similar to the dimeric form of Bacillus subtilis SecA and is 10 nm × 10 nm × 5 nm in size.
基金Supported by National Natural Science Foundation of China (11030042)
文摘A nuclear microprobe with high spatial resolution and high analyti- cal sensitivity was applied to analyze atmospheric aerosol at five monitoring sites in Shanghai city. Meantime, a new pattern recognition technique, which used the micro PIXE spectrum of a single aerosol particle as its fingerprint, was developed to identify the origin of the particle. The results showed that the major contributors to the at- mosphere pollution were soil dust (31.6%), building dust (30.8%), and the next were vehicle exhaust (13.7%), metallurgic industry excrements (5.6%), oil combustion (5%) and coal combustion (2.3%). Besides these, about 10% of the particles could not be identified. Based on the cluster analysis of these particles, they could be divided into eight groups. By inference, they might belong to some sub-pollution sources from soil dust, building dust and metallurgic industry excrements. Moreover, some new pollution sources from tyres and chemical plants were also revealed.
文摘Scanning electron microscope energy dispersive X ray analysis system (SEM EDX) is able to provide analytical data for each single particle. Hundreds of particles are usually analyzed for an aerosol sample. Both physical and chemical characterization can be performed in terms of particle size and particle class compositional data, respectively. Mineral dusts such as aluminosilicates and quartz particles as well as sea salt particles featured coarse fractions. Fine fractions were dominated by various sulfur containing and carbonaceous particles.
