对77例胃粘膜活检标本进行银染,显示核仁组成区相关的嗜银蛋白(Ag-NOR),进行计数和统计分析,并对银染标本作电镜观察及能谱分析。结果表明,Ag-NOR 颗粒计数的多少,对胃肿瘤恶性程度的诊断有重要参考价值。电镜证实银染物质定位于核仁组...对77例胃粘膜活检标本进行银染,显示核仁组成区相关的嗜银蛋白(Ag-NOR),进行计数和统计分析,并对银染标本作电镜观察及能谱分析。结果表明,Ag-NOR 颗粒计数的多少,对胃肿瘤恶性程度的诊断有重要参考价值。电镜证实银染物质定位于核仁组成区,银染阳性区能谱分析显示 Ag 峰,说明银染反应具特异性.展开更多
Zhang Zhi-Yi et al. demonstrated amphioxus immuno-positive reaction in Hatschek’s pit to antibodies to mammalian gonadotropins (LH and FSH). A gonadotropin substance in the homogenate of amphioxus’ Hatschek’s pit w...Zhang Zhi-Yi et al. demonstrated amphioxus immuno-positive reaction in Hatschek’s pit to antibodies to mammalian gonadotropins (LH and FSH). A gonadotropin substance in the homogenate of amphioxus’ Hatschek’s pit was found by Fang Yong-Qiang et al., who demonstrated that the substance existed in the epithelial cell of Hatschek’s pit by electron microscope. However, Sahline, in an immunohistochemical study using Hatschek’s pit of amphioxus, found no immunoreactive response to antibodies to pituitary hormones of vertebrate. Nozaki and Gorbman recently obtained evidence for gonadotropin activity in Hatschek’s pit of amphioxus (Branchiostoma betcheri) using antibodies to the beta subunit of human luteinizing hormone (hLH_β) and human chorionic gonadotropin (hCG)展开更多
Antisera against abseisic-acid-binding proteins(Ab Ⅰ)and anti-idiotypic antibodies to ABAmonoclonal antibody(Ab Ⅱ)have been developed and tested to comparatively localize abacisic-acid-bindingproteins(ABBPs)in maize...Antisera against abseisic-acid-binding proteins(Ab Ⅰ)and anti-idiotypic antibodies to ABAmonoclonal antibody(Ab Ⅱ)have been developed and tested to comparatively localize abacisic-acid-bindingproteins(ABBPs)in maize root tips using immunocytochemistry technique.Based on the staining pattern ob-served along the root tip,an apicobasal gradient of ABBP has been revealed with either Ab Ⅰ or Ab Ⅱ.Thelabels are mainly concentrated in,the root cap and apical meristem.In the elongation zone and root hair zone,only cells in pericycle and epidermis are obviously labeled.The silver labels are distributed evenly in thewhole cell of the apical meristem,but in the cells of the root cap and elongation zone,the positive stainingdegree in the cytoplasm is reduced even to completely negative staining;nevertheless,the nuclei and plasmamembranes are strongly labeled.As in the labeled cells of the root hair zone,only some immunoactive sitescan be seen in the nucleus compacted to the edge of the cells.The staining pattern in the whole root tip alsoshows obvious dependence of the ABBPs localization on the activity of the nucleus.The significance ofABBPs localization is discussed.展开更多
文摘对77例胃粘膜活检标本进行银染,显示核仁组成区相关的嗜银蛋白(Ag-NOR),进行计数和统计分析,并对银染标本作电镜观察及能谱分析。结果表明,Ag-NOR 颗粒计数的多少,对胃肿瘤恶性程度的诊断有重要参考价值。电镜证实银染物质定位于核仁组成区,银染阳性区能谱分析显示 Ag 峰,说明银染反应具特异性.
基金Project supported by the National Natural Science Foundation of China and Fujian Provincial Natural Science Foundation.
文摘Zhang Zhi-Yi et al. demonstrated amphioxus immuno-positive reaction in Hatschek’s pit to antibodies to mammalian gonadotropins (LH and FSH). A gonadotropin substance in the homogenate of amphioxus’ Hatschek’s pit was found by Fang Yong-Qiang et al., who demonstrated that the substance existed in the epithelial cell of Hatschek’s pit by electron microscope. However, Sahline, in an immunohistochemical study using Hatschek’s pit of amphioxus, found no immunoreactive response to antibodies to pituitary hormones of vertebrate. Nozaki and Gorbman recently obtained evidence for gonadotropin activity in Hatschek’s pit of amphioxus (Branchiostoma betcheri) using antibodies to the beta subunit of human luteinizing hormone (hLH_β) and human chorionic gonadotropin (hCG)
文摘Antisera against abseisic-acid-binding proteins(Ab Ⅰ)and anti-idiotypic antibodies to ABAmonoclonal antibody(Ab Ⅱ)have been developed and tested to comparatively localize abacisic-acid-bindingproteins(ABBPs)in maize root tips using immunocytochemistry technique.Based on the staining pattern ob-served along the root tip,an apicobasal gradient of ABBP has been revealed with either Ab Ⅰ or Ab Ⅱ.Thelabels are mainly concentrated in,the root cap and apical meristem.In the elongation zone and root hair zone,only cells in pericycle and epidermis are obviously labeled.The silver labels are distributed evenly in thewhole cell of the apical meristem,but in the cells of the root cap and elongation zone,the positive stainingdegree in the cytoplasm is reduced even to completely negative staining;nevertheless,the nuclei and plasmamembranes are strongly labeled.As in the labeled cells of the root hair zone,only some immunoactive sitescan be seen in the nucleus compacted to the edge of the cells.The staining pattern in the whole root tip alsoshows obvious dependence of the ABBPs localization on the activity of the nucleus.The significance ofABBPs localization is discussed.