Objective： To gain insight into the mechanism by which sex-determining region of Y chromosome （SRY）-related high-mobility-group box 2 （SOX2） involved in carcinogenesis and cancer stem cells （CSCs）. Data Sources...Objective： To gain insight into the mechanism by which sex-determining region of Y chromosome （SRY）-related high-mobility-group box 2 （SOX2） involved in carcinogenesis and cancer stem cells （CSCs）. Data Sources： The data used in this review were mainly published in English from 2000 to present obtained from PubMed. The search terms were ＂SOX2,＂ ＂cancer,＂ ＂tumor＂ or ＂CSCs.＂ Study Selection： Articles studying the mitochondria-related pathologic mechanism and treatment of glaucoma were selected and reviewed. Results： SOX2, a transcription factor that is the key in maintaining pluripotent properties of stem cells, is a member of SRV-related high-mobility group domain proteins. SOX2 participates in many biological processes, such as modulation of cell proliferation, regulation of cell death signaling, cell apoptosis, and most importantly, tumor formation and development. Although SOX2 has been implicated in the biology of various tumors and CSCs, the findings are highly controversial, and information regarding the underlying mechanism remains limited. Moreover, the mechanism by which SOX2 involved in carcinogenesis and tumor progression is rather unclear yet. Conclusions： Here, we review the important biological functions of SOX2 in different tumors and CSCs, and the function of SOX2 signaling in the pathobiology ofneoplasia, such as Wnt/β-catenin signaling pathway, Hippo signaling pathway, Survivin signaling pathway, P13K/Akt signaling pathway, and so on. Targeting towards SOX2 may be an effective therapeutic strategy for cancer therapy.展开更多
目的探讨胎儿游离DNA在无创性产前诊断中的应用价值。方法行产前诊断的孕妇87例,分为中期妊娠(16～24周)61例(中孕组)和早期妊娠(11～14周)26例(早孕组),采用实时荧光定量PCR方法,利用Taqman探针对妊娠早、中期孕妇血浆游离的胎儿Y染色...目的探讨胎儿游离DNA在无创性产前诊断中的应用价值。方法行产前诊断的孕妇87例,分为中期妊娠(16～24周)61例(中孕组)和早期妊娠(11～14周)26例(早孕组),采用实时荧光定量PCR方法,利用Taqman探针对妊娠早、中期孕妇血浆游离的胎儿Y染色体性别决定基因(sex-determining region of Y-chromosome,SRY)进行扩增和定量分析。结果孕13周后的样本均得到特异性扩增,特异度和灵敏度均为100%;中孕组男性胎儿SRY检测的平均DNA浓度为155.46copies/mL血浆,实测范围为43.6～326.8copies/mL血浆;早孕组平均浓度为26.42copies/mL血浆,实测范围为14.3～73.3copies/mL血浆。结论采用荧光实时定量PCR在妊娠早、中期可稳定检测到胎儿游离DNA,可作为孕期性连锁遗传性疾病研究或产前诊断的有效补充手段。展开更多
性发育异常在人类遗传性疾病中很常见,因此性别决定在临床和生物学研究中非常重要。Y染色体性别决定区(sex-determining region of Y-chromosome,Sry)即哺乳动物Y染色体上的睾丸决定基因片段,与性别决定密切相关。本文对Sry基因的结构...性发育异常在人类遗传性疾病中很常见,因此性别决定在临床和生物学研究中非常重要。Y染色体性别决定区(sex-determining region of Y-chromosome,Sry)即哺乳动物Y染色体上的睾丸决定基因片段,与性别决定密切相关。本文对Sry基因的结构功能和表达调节及其相关的性别决定分子机制进行了综述。展开更多
目的追踪骨髓间充质干细胞(bone mesenchymal stem cells,BMSCs)在溃疡性结肠炎大鼠模型体内的归巢情况,为BMSCs移植治疗溃疡性结肠炎提供实验基础。方法体外培养扩增Sprague-Dawley(SD)雄性大鼠BMSCs,流式细胞术鉴定BMSCs,采用慢病毒...目的追踪骨髓间充质干细胞(bone mesenchymal stem cells,BMSCs)在溃疡性结肠炎大鼠模型体内的归巢情况,为BMSCs移植治疗溃疡性结肠炎提供实验基础。方法体外培养扩增Sprague-Dawley(SD)雄性大鼠BMSCs,流式细胞术鉴定BMSCs,采用慢病毒载体介导的绿色荧光蛋白(green fluorescent protein,GFP)技术(lentivirus-GFP)对BMSCs进行荧光蛋白标记(Ad-GFP-BMSCs)。SD雌性大鼠被随机分为3组:空白组、模型组、Ad-GFP-BMSCs组。2,4,6-三硝基苯磺酸(2,4,6-trinitrobenzene sulfonic acid,TNBS)诱导溃疡性结肠炎,疾病活动指数(disease activity index,DAI)对各组大鼠进行评估,采用性别交叉移植的方法,尾静脉注射Ad-GFP-BMSCs于雌性大鼠体内,1周后收集结肠标本,对结肠标本进行病理学评估,免疫荧光检测结肠组织GFP表达,PCR检测Y染色体的性别决定区(sex-determining region on the Y chromosome,SRY)基因。结果 TNBS能成功诱导溃疡性结肠炎,与模型组相比,Ad-GFP-BMSCs组DAI评分显著下降(P<0.05)。Lentivirus-GFP成功标记SD大鼠的BMSCs,转染后BMSCs能够稳定表达GFP蛋白,尾静脉注射1周,Ad-GFP-BMSCs组免疫荧光可检测到GFP表达,PCR可检测出SRY基因,空白组和模型组无GFP和SRY基因表达。结论慢病毒载体介导的GFP转染技术和SRY基因可以追踪移植的BMSCs,而且BMSCs通过尾静脉注射可以归巢于受损的结肠组织,这可能为BMSCs移植治疗溃疡性结肠炎提供实验基础。展开更多
基金This study was supported by grants from the National Natural Science Foundation of China （No. 81172234） and the Fundamental Research Funds for the Central Universities of China.
文摘Objective： To gain insight into the mechanism by which sex-determining region of Y chromosome （SRY）-related high-mobility-group box 2 （SOX2） involved in carcinogenesis and cancer stem cells （CSCs）. Data Sources： The data used in this review were mainly published in English from 2000 to present obtained from PubMed. The search terms were ＂SOX2,＂ ＂cancer,＂ ＂tumor＂ or ＂CSCs.＂ Study Selection： Articles studying the mitochondria-related pathologic mechanism and treatment of glaucoma were selected and reviewed. Results： SOX2, a transcription factor that is the key in maintaining pluripotent properties of stem cells, is a member of SRV-related high-mobility group domain proteins. SOX2 participates in many biological processes, such as modulation of cell proliferation, regulation of cell death signaling, cell apoptosis, and most importantly, tumor formation and development. Although SOX2 has been implicated in the biology of various tumors and CSCs, the findings are highly controversial, and information regarding the underlying mechanism remains limited. Moreover, the mechanism by which SOX2 involved in carcinogenesis and tumor progression is rather unclear yet. Conclusions： Here, we review the important biological functions of SOX2 in different tumors and CSCs, and the function of SOX2 signaling in the pathobiology ofneoplasia, such as Wnt/β-catenin signaling pathway, Hippo signaling pathway, Survivin signaling pathway, P13K/Akt signaling pathway, and so on. Targeting towards SOX2 may be an effective therapeutic strategy for cancer therapy.
文摘目的探讨胎儿游离DNA在无创性产前诊断中的应用价值。方法行产前诊断的孕妇87例,分为中期妊娠(16～24周)61例(中孕组)和早期妊娠(11～14周)26例(早孕组),采用实时荧光定量PCR方法,利用Taqman探针对妊娠早、中期孕妇血浆游离的胎儿Y染色体性别决定基因(sex-determining region of Y-chromosome,SRY)进行扩增和定量分析。结果孕13周后的样本均得到特异性扩增,特异度和灵敏度均为100%;中孕组男性胎儿SRY检测的平均DNA浓度为155.46copies/mL血浆,实测范围为43.6～326.8copies/mL血浆;早孕组平均浓度为26.42copies/mL血浆,实测范围为14.3～73.3copies/mL血浆。结论采用荧光实时定量PCR在妊娠早、中期可稳定检测到胎儿游离DNA,可作为孕期性连锁遗传性疾病研究或产前诊断的有效补充手段。
文摘性发育异常在人类遗传性疾病中很常见,因此性别决定在临床和生物学研究中非常重要。Y染色体性别决定区(sex-determining region of Y-chromosome,Sry)即哺乳动物Y染色体上的睾丸决定基因片段,与性别决定密切相关。本文对Sry基因的结构功能和表达调节及其相关的性别决定分子机制进行了综述。
文摘目的追踪骨髓间充质干细胞(bone mesenchymal stem cells,BMSCs)在溃疡性结肠炎大鼠模型体内的归巢情况,为BMSCs移植治疗溃疡性结肠炎提供实验基础。方法体外培养扩增Sprague-Dawley(SD)雄性大鼠BMSCs,流式细胞术鉴定BMSCs,采用慢病毒载体介导的绿色荧光蛋白(green fluorescent protein,GFP)技术(lentivirus-GFP)对BMSCs进行荧光蛋白标记(Ad-GFP-BMSCs)。SD雌性大鼠被随机分为3组:空白组、模型组、Ad-GFP-BMSCs组。2,4,6-三硝基苯磺酸(2,4,6-trinitrobenzene sulfonic acid,TNBS)诱导溃疡性结肠炎,疾病活动指数(disease activity index,DAI)对各组大鼠进行评估,采用性别交叉移植的方法,尾静脉注射Ad-GFP-BMSCs于雌性大鼠体内,1周后收集结肠标本,对结肠标本进行病理学评估,免疫荧光检测结肠组织GFP表达,PCR检测Y染色体的性别决定区(sex-determining region on the Y chromosome,SRY)基因。结果 TNBS能成功诱导溃疡性结肠炎,与模型组相比,Ad-GFP-BMSCs组DAI评分显著下降(P<0.05)。Lentivirus-GFP成功标记SD大鼠的BMSCs,转染后BMSCs能够稳定表达GFP蛋白,尾静脉注射1周,Ad-GFP-BMSCs组免疫荧光可检测到GFP表达,PCR可检测出SRY基因,空白组和模型组无GFP和SRY基因表达。结论慢病毒载体介导的GFP转染技术和SRY基因可以追踪移植的BMSCs,而且BMSCs通过尾静脉注射可以归巢于受损的结肠组织,这可能为BMSCs移植治疗溃疡性结肠炎提供实验基础。
