Objective To evaluate the effects of ethyl-acetate fraction （EAF） of extracts from Tetrastigma hemsleyanum Diels et. Gilg （TDG） on immune functions of ICR mice. Methods ICR mice were exposed to different doses of ...Objective To evaluate the effects of ethyl-acetate fraction （EAF） of extracts from Tetrastigma hemsleyanum Diels et. Gilg （TDG） on immune functions of ICR mice. Methods ICR mice were exposed to different doses of EAF for 15 or 30 days and then their immune functions were analyzed, including ConA-induced splenic lymphocyte transformation, SRBC- induced delayed type hypersensitivity response, serum hemolysin analysis, antibody-producing cells, peritoneal macrophage phagocytized chicken red blood cells, natural killer cell activity, and serum level of cytoldnes. Results EAF of extracts from TDG at different doses had various effects on immune functions of ICR mice. As compared with the controls, it increased the mouse spleen lymphocyte transformation induced by ConA, the left-hind voix pedis thickness and the number of plague forming cells （PFCs） at the dose of 1.82 mg/mL, 5.48 mg/mL, and 9.12 mg/mL, respectively; increased the ink clearance ability at the dose of 0.91 mg/mL, 1.82 mg/mL, 5.48 mg/mL, and 9.12 mg/mL, respectively; increased the phagocytosis index of mononuclear-macrophages and production of serum interferon-gamma （IFN-？） at the dose of 5.48 mg/mL; and could promote the production of serum tumor necrosis factor-alpha （TNF-α） at the dose of 9.12 mg/mL. Conclusion EAF of extracts from TDG can regulate mouse immune functions in vivo.展开更多
目的:观察靳三针联合鼠神经生长因子治疗小儿痉挛型脑瘫的临床疗效。方法:选取132例痉挛型脑瘫患儿按随机数表法分为对照组与观察组。对照组采用鼠神经生长因子治疗,观察组采用靳三针联合鼠神经生长因子治疗。比较两组治疗前后运动功能...目的:观察靳三针联合鼠神经生长因子治疗小儿痉挛型脑瘫的临床疗效。方法:选取132例痉挛型脑瘫患儿按随机数表法分为对照组与观察组。对照组采用鼠神经生长因子治疗,观察组采用靳三针联合鼠神经生长因子治疗。比较两组治疗前后运动功能、痉挛程度、血清白细胞介素-6(interleukin-6,IL-6)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)水平的变化情况,并对两组临床疗效进行评价。结果:观察组有效率为90.91%,显著优于对照组75.76%,差异有统计学意义(P<0.05);与治疗前比较,两组粗大运动功能评估量表(gross motor function measure,GMFM)、精细运动功能评定量表(peabody Development Motor Scales-2,PDMS-2)评分均上升,而改良Ashworth痉挛量表(modified ashworth scale,MAS)积分、血清IL-6、TNF-α水平均下降,且观察组均优于对照组,差异具有统计学意义(P<0.05);治疗期间两组均未出现不良反应及肝肾功能异常。结论:采用靳三针联合鼠神经生长因子治疗小儿痉挛型脑瘫可显著提高临床疗效,改善患儿运动功能,减轻痉挛程度,降低血清IL-6、TNF-α水平,促进患儿身体恢复。展开更多
基金the Scientific Research Foundation of Health Bureau of Zhejiang Province (No. 2006c151)
文摘Objective To evaluate the effects of ethyl-acetate fraction （EAF） of extracts from Tetrastigma hemsleyanum Diels et. Gilg （TDG） on immune functions of ICR mice. Methods ICR mice were exposed to different doses of EAF for 15 or 30 days and then their immune functions were analyzed, including ConA-induced splenic lymphocyte transformation, SRBC- induced delayed type hypersensitivity response, serum hemolysin analysis, antibody-producing cells, peritoneal macrophage phagocytized chicken red blood cells, natural killer cell activity, and serum level of cytoldnes. Results EAF of extracts from TDG at different doses had various effects on immune functions of ICR mice. As compared with the controls, it increased the mouse spleen lymphocyte transformation induced by ConA, the left-hind voix pedis thickness and the number of plague forming cells （PFCs） at the dose of 1.82 mg/mL, 5.48 mg/mL, and 9.12 mg/mL, respectively; increased the ink clearance ability at the dose of 0.91 mg/mL, 1.82 mg/mL, 5.48 mg/mL, and 9.12 mg/mL, respectively; increased the phagocytosis index of mononuclear-macrophages and production of serum interferon-gamma （IFN-？） at the dose of 5.48 mg/mL; and could promote the production of serum tumor necrosis factor-alpha （TNF-α） at the dose of 9.12 mg/mL. Conclusion EAF of extracts from TDG can regulate mouse immune functions in vivo.
文摘目的:观察靳三针联合鼠神经生长因子治疗小儿痉挛型脑瘫的临床疗效。方法:选取132例痉挛型脑瘫患儿按随机数表法分为对照组与观察组。对照组采用鼠神经生长因子治疗,观察组采用靳三针联合鼠神经生长因子治疗。比较两组治疗前后运动功能、痉挛程度、血清白细胞介素-6(interleukin-6,IL-6)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)水平的变化情况,并对两组临床疗效进行评价。结果:观察组有效率为90.91%,显著优于对照组75.76%,差异有统计学意义(P<0.05);与治疗前比较,两组粗大运动功能评估量表(gross motor function measure,GMFM)、精细运动功能评定量表(peabody Development Motor Scales-2,PDMS-2)评分均上升,而改良Ashworth痉挛量表(modified ashworth scale,MAS)积分、血清IL-6、TNF-α水平均下降,且观察组均优于对照组,差异具有统计学意义(P<0.05);治疗期间两组均未出现不良反应及肝肾功能异常。结论:采用靳三针联合鼠神经生长因子治疗小儿痉挛型脑瘫可显著提高临床疗效,改善患儿运动功能,减轻痉挛程度,降低血清IL-6、TNF-α水平,促进患儿身体恢复。
