目的:考察用合煎、分煎2种方法制备的香薷散中香荆芥酚和麝香草酚含量的变化并比较其体外抗菌作用。方法:(1)有效成分含量测定用HPLC法,采用Phenomenex Luna C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-5%乙酸水(60∶40)为流动相,流速1...目的:考察用合煎、分煎2种方法制备的香薷散中香荆芥酚和麝香草酚含量的变化并比较其体外抗菌作用。方法:(1)有效成分含量测定用HPLC法,采用Phenomenex Luna C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-5%乙酸水(60∶40)为流动相,流速1.0 mL·min-1,检测波长274nm,柱温30℃;(2)采用微量稀释法体外测试香薷散不同煎液对常见菌种的最低抑菌浓度。结果:(1)合煎液中香荆芥酚和麝香草酚的平均含量高于分煎液;(2)合煎液对金黄色葡萄球菌、乙型溶血性链球菌和绿脓杆菌的抑菌作用大于分煎液。结论:与分煎液比较,合煎液中香荆芥酚和麝香草酚的含量较高,抑菌作用较强。展开更多
[Objectives]To investigate the effect and mechanism of Dachengqi Decoction and separated decoction on incomplete intestinal obstruction in rats.[Methods]80 healthy SD rats were selected to establish incomplete intesti...[Objectives]To investigate the effect and mechanism of Dachengqi Decoction and separated decoction on incomplete intestinal obstruction in rats.[Methods]80 healthy SD rats were selected to establish incomplete intestinal obstruction model by silk ligation.The dosage was 20 mL/kg for 3 d,and the damage index of ileocecal mucosa was analyzed;the morphology of ileocecal mucosa was observed by HE staining;the serum levels of IL-1α,IL-1β,IL-6,IL-18,Ach,NO,ET,IL-1,TNF-αand ultra-micro Na+-K+-ATPase were detected by ELISA.[Results]Compared with the model group,the mucosal damage index of Dachengqi Decoction and each separated decoction group decreased significantly(P<0.05);compared with the normal group and sham operation group,the serum level of IL-1,IL-6,TNF-αand other factors in the model group increased significantly(P<0.05);compared with the model group,the serum IL-1,IL-6 and TNF-αsecretion levels of rats in Dachengqi Decoction group and separated decoction group decreased(P<0.01).[Conclusions]Dachengqi Decoction and each separated decoction can effectively improve intestinal tissue pathological damage in the incomplete intestinal obstruction model rats,and reduce the inflammatory reaction in the rat body.展开更多
基金2023 Young and Middle-aged University Teachers Basic Scientific Research Ability Improvement Project in Guangxi(2023KY0299)High-level Key Discipline Construction Project of Traditional Chinese Medicine of National Administration of Traditional Chinese Medicine(zyyzdxk-2023165)+3 种基金Talent Training Project of Guangxi International Zhuang Medicine Hospital—"Young Seedling Project"(2022001)Guangxi Traditional Chinese Medicine Multidisciplinary and Interdisciplinary Innovation Team Project(GZKJ2309)High-level Talent Cultivation Innovation Team Funding Project of Guangxi University of Chinese Medicine(2022A008)2023 Three-Year Action Plan Project for High-Level Talent Team Construction of Guangxi International Zhuang Medicine Hospital(GZCX20231203,GZCX20231202).
文摘[Objectives]To investigate the effect and mechanism of Dachengqi Decoction and separated decoction on incomplete intestinal obstruction in rats.[Methods]80 healthy SD rats were selected to establish incomplete intestinal obstruction model by silk ligation.The dosage was 20 mL/kg for 3 d,and the damage index of ileocecal mucosa was analyzed;the morphology of ileocecal mucosa was observed by HE staining;the serum levels of IL-1α,IL-1β,IL-6,IL-18,Ach,NO,ET,IL-1,TNF-αand ultra-micro Na+-K+-ATPase were detected by ELISA.[Results]Compared with the model group,the mucosal damage index of Dachengqi Decoction and each separated decoction group decreased significantly(P<0.05);compared with the normal group and sham operation group,the serum level of IL-1,IL-6,TNF-αand other factors in the model group increased significantly(P<0.05);compared with the model group,the serum IL-1,IL-6 and TNF-αsecretion levels of rats in Dachengqi Decoction group and separated decoction group decreased(P<0.01).[Conclusions]Dachengqi Decoction and each separated decoction can effectively improve intestinal tissue pathological damage in the incomplete intestinal obstruction model rats,and reduce the inflammatory reaction in the rat body.
