为了进一步研究微生物富硒的能力,得到富硒能力较强的啤酒酵母,以啤酒酵母WX-01为出发菌,通过高浓度亚硒酸钠初筛,再经过常压室温等离子体(atmospheric and room temperature plasma,ARTP)诱变处理以及亚硒酸钠抗性平板筛选,观察菌株的...为了进一步研究微生物富硒的能力,得到富硒能力较强的啤酒酵母,以啤酒酵母WX-01为出发菌,通过高浓度亚硒酸钠初筛,再经过常压室温等离子体(atmospheric and room temperature plasma,ARTP)诱变处理以及亚硒酸钠抗性平板筛选,观察菌株的生长状况结合对其生物量与硒含量的测定,选育出一株富硒优势啤酒酵母。通过培养条件为添加质量浓度35 mg/L,加硒时间8 h,培养时间36 h,得到的酵母WX-1的生物量提高到(5192±36)mg/L,较原始菌株WX-01提高了201%,硒含量达到(1475±33)μg/g,较原始菌株提高了330%,其有机硒产量和转化率分别为7658μg/L和97.1%。扫描电镜分析酵母菌富集后表面有少量单质硒析出。另外红外光谱在特定区域出现不同强度的吸收峰表明酵母细胞参与了硒蛋白的合成。展开更多
With the improvement of living standards, people pay more attention to the agricultural products with health protection function, and the selenium-rich agricultural products attract more and more consumers. The main b...With the improvement of living standards, people pay more attention to the agricultural products with health protection function, and the selenium-rich agricultural products attract more and more consumers. The main biological role of selenium is to resist oxidation and inflammatory response, mainly focusing on resisting aging, preventing cardiovascular disease, protecting eyesight, counteracting or destroying the toxic properties, preventing cancer and thyroid disease. In most areas of China, there is a widespread shortage of selenium, thus producing selenium-rich agricultural products to provide natural selenium-rich health food to the areas in need of selenium, has gradually become a new hot spot of China's health food industry, but high content of selenium in food is detrimental to human body, even leads to selenium intoxication, and artificially adding inorganic selenium is difficult to guarantee that the selenium content of agricultural products is not exceeded. According to human body's daily demand for selenium in dietetics and the content of selenium in agricultural products in the Chinese food composition table, we put forward the recommendations on the standard of selenium in agricultural products, in order to provide the basis for China to formulate the health standard of selenium content in selenium-rich agricultural products.展开更多
文摘为了进一步研究微生物富硒的能力,得到富硒能力较强的啤酒酵母,以啤酒酵母WX-01为出发菌,通过高浓度亚硒酸钠初筛,再经过常压室温等离子体(atmospheric and room temperature plasma,ARTP)诱变处理以及亚硒酸钠抗性平板筛选,观察菌株的生长状况结合对其生物量与硒含量的测定,选育出一株富硒优势啤酒酵母。通过培养条件为添加质量浓度35 mg/L,加硒时间8 h,培养时间36 h,得到的酵母WX-1的生物量提高到(5192±36)mg/L,较原始菌株WX-01提高了201%,硒含量达到(1475±33)μg/g,较原始菌株提高了330%,其有机硒产量和转化率分别为7658μg/L和97.1%。扫描电镜分析酵母菌富集后表面有少量单质硒析出。另外红外光谱在特定区域出现不同强度的吸收峰表明酵母细胞参与了硒蛋白的合成。
基金Supported by Hunan University Innovation Team FoundationHunan Science and Technology Plan Project (2011NK3093)
文摘With the improvement of living standards, people pay more attention to the agricultural products with health protection function, and the selenium-rich agricultural products attract more and more consumers. The main biological role of selenium is to resist oxidation and inflammatory response, mainly focusing on resisting aging, preventing cardiovascular disease, protecting eyesight, counteracting or destroying the toxic properties, preventing cancer and thyroid disease. In most areas of China, there is a widespread shortage of selenium, thus producing selenium-rich agricultural products to provide natural selenium-rich health food to the areas in need of selenium, has gradually become a new hot spot of China's health food industry, but high content of selenium in food is detrimental to human body, even leads to selenium intoxication, and artificially adding inorganic selenium is difficult to guarantee that the selenium content of agricultural products is not exceeded. According to human body's daily demand for selenium in dietetics and the content of selenium in agricultural products in the Chinese food composition table, we put forward the recommendations on the standard of selenium in agricultural products, in order to provide the basis for China to formulate the health standard of selenium content in selenium-rich agricultural products.
