BACKGROUND The rate of positive tests using fecal immunochemical test(FIT)does not decrease with subsequent campaigns,but the positive predictive value of advanced neoplasia significantly decreases in subsequent campa...BACKGROUND The rate of positive tests using fecal immunochemical test(FIT)does not decrease with subsequent campaigns,but the positive predictive value of advanced neoplasia significantly decreases in subsequent campaign after a first negative test.A relationship between the fecal hemoglobin concentration(Fhb)and the opportunity to detect a colorectal cancer in subsequent campaign has been shown.AIM To predict the severity of colorectal lesions based on Fhb measured during previous colorectal cancer screening campaign.METHODS This etiological study included 293750 patients aged 50-74,living in Auvergne-Rhone-Alpes(France).These patients completed at least two FIT[test_((-1))and test_((0))]between June 2015 and December 2019.Delay between test_((-1))and test_((0))was>1year and test_((-1))result was negative(<150 ngHb/mL).The severity of colorectal lesions diagnosed at test_((0))was described according to Fhb measured at test_((-1))[Fhb_((-1))].The relationship between the severity classified in seven ordinal categories and the predictive factors was analyzed in an ordered multivariate polytomous regression model.RESULTS The test_((0))positive rate was 4.0%,and the colonoscopy completion rate was 97.1%in 11594 patients who showed a positive test_((0)).The colonoscopy detection rate was 77.7%in those 11254 patients who underwent a colonoscopy.A total of 8748 colorectal lesions were detected(including 2182 low-risk-polyps,2400 high-riskpolyp,and 502 colorectal cancer).The colonoscopy detection rate varied significantly with Fhb_((-1))[0 ngHb/mL:75.6%,(0-50 ngHb/mL):77.3%,(50-100 ngHb/mL):88.7%,(100-150 ngHb/mL):90.3%;P=0.001].People with a Fhb_((-1))within(100-150 ngHb/mL)(P=0.001)were 2.6(2.2;3.0)times more likely to have a high severity level compared to those having a Fhb_((-1))value of zero.This risk was reduced by 20%in patients aged 55-59 compared to those aged<55[adjusted odds ratio:0.8(0.6;1.0)].CONCLUSION The study showed that higher Fhb_((-1))is correlated to an increased risk of severity of colorectal lesi展开更多
Cultivating microalgae in post hydrothermal liquefaction wastewater(PHWW)offers many benefits,including nutrients recovery and reuse,wastewater purification and biomass production.However,the high nutrients concentrat...Cultivating microalgae in post hydrothermal liquefaction wastewater(PHWW)offers many benefits,including nutrients recovery and reuse,wastewater purification and biomass production.However,the high nutrients concentration and toxic substances in PHWW undermine the efficiency of biomass production and nutrient recovery.This study aimed to investigate the effects of the microalgae strains,initial nutrients concentrations and inoculum sizes on biomass production and nutrient recovery using PHWW as the cultivation medium.Results indicated that both biomass production and nutrients recovery were successfully improved by using the screened microalgae strain at the desirable initial nutrient concentration with the suggested algae inoculum size.Chlorella vulgaris 1067 probably demonstrated the strongest tolerance ability among the five microalgae strains screened,and performed well in the diluted PHWW,of which initial TN concentration was approximately 500 mg/L.The desirable inoculum size was determined to be 0.103-0.135 g/L.The biomass daily productivity was increased by 15.67-fold(reached 0.13 g/(L·d)).With the above optimal conditions,high biomass production and nutrient recovery from the PHWW to produce microalgae biomass for bioenergy production were achieved.展开更多
目的：建立糖原磷酸化酶（GPa）抑制剂的体外高通量筛选模型。方法：用梯度离心方法提取大鼠肝脏GPa，以葡萄糖-1-磷酸作为底物，通过测定反应中磷酸根的释放量，间接反映肝脏GPa的活性。通过对反应体系的优化，调整反应条件，建立96孔...目的：建立糖原磷酸化酶（GPa）抑制剂的体外高通量筛选模型。方法：用梯度离心方法提取大鼠肝脏GPa，以葡萄糖-1-磷酸作为底物，通过测定反应中磷酸根的释放量，间接反映肝脏GPa的活性。通过对反应体系的优化，调整反应条件，建立96孔微孔板的高通量筛选模型，用咖啡因对此模型进行验证，并评价高通量技术参数[Z＇-因子（Z＇-factor），信号本底比（signal to background，S／B），信噪比（signal to noise，S／N）]。用此模型对5000个样品（包括合成化合物、天然提取物）组成的随机库进行体外筛选，考察这些样品对GPa的抑制作用。结果：确定反应体系条件是：反应温度25℃，反应时间30min，底物浓度0．5mmol·L^-1，大鼠肝脏GPa的用量为250ng。用此条件测定咖啡因对GPa的抑制曲线，计算其半数抑制浓度（IC50）为（285．3±39．6）μmol·L^-1，与文献报道（IC50=240μmol·L^-1）基本一致；本模型技术参数Z＇-因子=0．79，S／B=0．45，S／N=11．32，表明此模型可以用于高通量筛选。应用此模型筛选得到有活性的化合物9个，其IC50在3．56～45．75μmol·L^-1。结论：建立的体外GPa抑制剂高通量筛选模型具有快速、微量、准确的特点，可以作为研究降糖药的工具。展开更多
文摘BACKGROUND The rate of positive tests using fecal immunochemical test(FIT)does not decrease with subsequent campaigns,but the positive predictive value of advanced neoplasia significantly decreases in subsequent campaign after a first negative test.A relationship between the fecal hemoglobin concentration(Fhb)and the opportunity to detect a colorectal cancer in subsequent campaign has been shown.AIM To predict the severity of colorectal lesions based on Fhb measured during previous colorectal cancer screening campaign.METHODS This etiological study included 293750 patients aged 50-74,living in Auvergne-Rhone-Alpes(France).These patients completed at least two FIT[test_((-1))and test_((0))]between June 2015 and December 2019.Delay between test_((-1))and test_((0))was>1year and test_((-1))result was negative(<150 ngHb/mL).The severity of colorectal lesions diagnosed at test_((0))was described according to Fhb measured at test_((-1))[Fhb_((-1))].The relationship between the severity classified in seven ordinal categories and the predictive factors was analyzed in an ordered multivariate polytomous regression model.RESULTS The test_((0))positive rate was 4.0%,and the colonoscopy completion rate was 97.1%in 11594 patients who showed a positive test_((0)).The colonoscopy detection rate was 77.7%in those 11254 patients who underwent a colonoscopy.A total of 8748 colorectal lesions were detected(including 2182 low-risk-polyps,2400 high-riskpolyp,and 502 colorectal cancer).The colonoscopy detection rate varied significantly with Fhb_((-1))[0 ngHb/mL:75.6%,(0-50 ngHb/mL):77.3%,(50-100 ngHb/mL):88.7%,(100-150 ngHb/mL):90.3%;P=0.001].People with a Fhb_((-1))within(100-150 ngHb/mL)(P=0.001)were 2.6(2.2;3.0)times more likely to have a high severity level compared to those having a Fhb_((-1))value of zero.This risk was reduced by 20%in patients aged 55-59 compared to those aged<55[adjusted odds ratio:0.8(0.6;1.0)].CONCLUSION The study showed that higher Fhb_((-1))is correlated to an increased risk of severity of colorectal lesi
基金the National Natural Science Foundation of China(51576206,51308535)Beijing Science and Technology Program(Z161100001316009).
文摘Cultivating microalgae in post hydrothermal liquefaction wastewater(PHWW)offers many benefits,including nutrients recovery and reuse,wastewater purification and biomass production.However,the high nutrients concentration and toxic substances in PHWW undermine the efficiency of biomass production and nutrient recovery.This study aimed to investigate the effects of the microalgae strains,initial nutrients concentrations and inoculum sizes on biomass production and nutrient recovery using PHWW as the cultivation medium.Results indicated that both biomass production and nutrients recovery were successfully improved by using the screened microalgae strain at the desirable initial nutrient concentration with the suggested algae inoculum size.Chlorella vulgaris 1067 probably demonstrated the strongest tolerance ability among the five microalgae strains screened,and performed well in the diluted PHWW,of which initial TN concentration was approximately 500 mg/L.The desirable inoculum size was determined to be 0.103-0.135 g/L.The biomass daily productivity was increased by 15.67-fold(reached 0.13 g/(L·d)).With the above optimal conditions,high biomass production and nutrient recovery from the PHWW to produce microalgae biomass for bioenergy production were achieved.
文摘目的：建立糖原磷酸化酶（GPa）抑制剂的体外高通量筛选模型。方法：用梯度离心方法提取大鼠肝脏GPa，以葡萄糖-1-磷酸作为底物，通过测定反应中磷酸根的释放量，间接反映肝脏GPa的活性。通过对反应体系的优化，调整反应条件，建立96孔微孔板的高通量筛选模型，用咖啡因对此模型进行验证，并评价高通量技术参数[Z＇-因子（Z＇-factor），信号本底比（signal to background，S／B），信噪比（signal to noise，S／N）]。用此模型对5000个样品（包括合成化合物、天然提取物）组成的随机库进行体外筛选，考察这些样品对GPa的抑制作用。结果：确定反应体系条件是：反应温度25℃，反应时间30min，底物浓度0．5mmol·L^-1，大鼠肝脏GPa的用量为250ng。用此条件测定咖啡因对GPa的抑制曲线，计算其半数抑制浓度（IC50）为（285．3±39．6）μmol·L^-1，与文献报道（IC50=240μmol·L^-1）基本一致；本模型技术参数Z＇-因子=0．79，S／B=0．45，S／N=11．32，表明此模型可以用于高通量筛选。应用此模型筛选得到有活性的化合物9个，其IC50在3．56～45．75μmol·L^-1。结论：建立的体外GPa抑制剂高通量筛选模型具有快速、微量、准确的特点，可以作为研究降糖药的工具。
