目的探究雄黄主要成分二硫化二砷(As_(2)S_(2))对三阴性乳腺癌(triple negative breast cancer,TNBC)的作用及表观遗传调控机制。方法采用CCK-8、平板克隆形成和细胞划痕实验探究As_(2)S_(2)对人正常乳腺上皮细胞MCF-10A及TNBC细胞增殖...目的探究雄黄主要成分二硫化二砷(As_(2)S_(2))对三阴性乳腺癌(triple negative breast cancer,TNBC)的作用及表观遗传调控机制。方法采用CCK-8、平板克隆形成和细胞划痕实验探究As_(2)S_(2)对人正常乳腺上皮细胞MCF-10A及TNBC细胞增殖和迁移的影响;4D-label free定量蛋白质组学分析挖掘As_(2)S_(2)抗TNBC的潜在干预靶点酸性核磷蛋白家族成员32A(acidic nuclear phosphoprotein family member 32A,ANP32A);慢病毒感染法构建ANP32A过表达敲低细胞株,探究潜在靶点ANP32A对As_(2)S_(2)抗TNBC作用的影响;蛋白质免疫共沉淀和Western blot实验探究As_(2)S_(2)是否通过ANP32A调控TNBC细胞H3乙酰化。结果As_(2)S_(2)对人正常乳腺上皮细胞MCF-10A影响甚微,但显著抑制TNBC细胞增殖和迁移,且呈剂量依赖性;4D-lable free定量蛋白质组学分析结果显示,促癌因子ANP32A被As_(2)S_(2)显著下调,且ANP32A表达影响As_(2)S_(2)在TNBC中的抗增殖和迁移效果。As_(2)S_(2)能下调ANP32A的蛋白水平,抑制乙酰转移酶抑制剂复合物亚基的招募,增加H3乙酰化水平。结论As_(2)S_(2)通过下调ANP32A蛋白调控TNBC细胞中H3乙酰化,抑制TNBC细胞增殖和迁移。展开更多
Based on the cores from 47 wells and logging data of 130 wells,the main types of sand bodies in the shallow water delta of the Chang 8_(2) sub-member in the Triassic Yanchang Formation,Longdong Area,Ordos Basin were c...Based on the cores from 47 wells and logging data of 130 wells,the main types of sand bodies in the shallow water delta of the Chang 8_(2) sub-member in the Triassic Yanchang Formation,Longdong Area,Ordos Basin were comprehensively analyzed,the distribution characteristics of sand bodies more than 20 m thick were identified,and the genetic types of the thick sand bodies were sorted out.In this region,thick-bed sand bodies can be divided into two types according to the shape and position:type 1 is the sand body in wide strip and is distributed between the average high water mark and the average low water mark;type 2 is distributed on both sides of the average low water mark and is in irregular lump shape.Based on the principle of the volume distribution of sediments and the change rule of accommodation space,the genetic models of two types of thick-bed sand bodies in the Chang 8_(2) sub-member are superimposed distributary channel sand bodies in high accommodation space and superimposed composite sand bodies in low accommodation space.展开更多
文摘目的探究雄黄主要成分二硫化二砷(As_(2)S_(2))对三阴性乳腺癌(triple negative breast cancer,TNBC)的作用及表观遗传调控机制。方法采用CCK-8、平板克隆形成和细胞划痕实验探究As_(2)S_(2)对人正常乳腺上皮细胞MCF-10A及TNBC细胞增殖和迁移的影响;4D-label free定量蛋白质组学分析挖掘As_(2)S_(2)抗TNBC的潜在干预靶点酸性核磷蛋白家族成员32A(acidic nuclear phosphoprotein family member 32A,ANP32A);慢病毒感染法构建ANP32A过表达敲低细胞株,探究潜在靶点ANP32A对As_(2)S_(2)抗TNBC作用的影响;蛋白质免疫共沉淀和Western blot实验探究As_(2)S_(2)是否通过ANP32A调控TNBC细胞H3乙酰化。结果As_(2)S_(2)对人正常乳腺上皮细胞MCF-10A影响甚微,但显著抑制TNBC细胞增殖和迁移,且呈剂量依赖性;4D-lable free定量蛋白质组学分析结果显示,促癌因子ANP32A被As_(2)S_(2)显著下调,且ANP32A表达影响As_(2)S_(2)在TNBC中的抗增殖和迁移效果。As_(2)S_(2)能下调ANP32A的蛋白水平,抑制乙酰转移酶抑制剂复合物亚基的招募,增加H3乙酰化水平。结论As_(2)S_(2)通过下调ANP32A蛋白调控TNBC细胞中H3乙酰化,抑制TNBC细胞增殖和迁移。
基金Supported by the National Basic Research Program of China (973 Program)(2014CB239000)China Petroleum Scientific Research and Technology Development Project (YJXK2019-16)。
文摘Based on the cores from 47 wells and logging data of 130 wells,the main types of sand bodies in the shallow water delta of the Chang 8_(2) sub-member in the Triassic Yanchang Formation,Longdong Area,Ordos Basin were comprehensively analyzed,the distribution characteristics of sand bodies more than 20 m thick were identified,and the genetic types of the thick sand bodies were sorted out.In this region,thick-bed sand bodies can be divided into two types according to the shape and position:type 1 is the sand body in wide strip and is distributed between the average high water mark and the average low water mark;type 2 is distributed on both sides of the average low water mark and is in irregular lump shape.Based on the principle of the volume distribution of sediments and the change rule of accommodation space,the genetic models of two types of thick-bed sand bodies in the Chang 8_(2) sub-member are superimposed distributary channel sand bodies in high accommodation space and superimposed composite sand bodies in low accommodation space.
