Objective: To study the significance of E-selectin andits ligand-sLeX in the metastasis of hepatocellularcarcinoma (HCC).Methods: Flow cytometry and immunohistochemistrywere used to detect the expression of E-selectin...Objective: To study the significance of E-selectin andits ligand-sLeX in the metastasis of hepatocellularcarcinoma (HCC).Methods: Flow cytometry and immunohistochemistrywere used to detect the expression of E-selectin and itsligand-sLeX in both HCC cell lines and human HCCtissues.Results: The positive rate of E-selectin in vascular en-dothelial cells adjacent to cancer was 67.9% (19/28). Theexpression of E-selectin in tumors accompanied withemboli or satellite foci was significantly higher than thatin tumors without emboli or satellite foci (P<0.05),and it was not related to tumor size, tumor capsule,AFP content, and the degree of differentiation. Thepositive expression of sLeX in SMMU-7721, PLF/PRF/5 and HepGⅡ cell lines was 7.03%, 63.35% and97.29% respectively. The positive cells of sLeX mainlydistributed in the margin of tumor tissues. The positiveexpression of sLeX in HCC cells in emboli or invasivetumor tissues was much higher than in primary foci.Conclusion: E-selectin and its ligand-sLeX are closelycorrelated with the metastasis of HCC.展开更多
目的探讨大肠肿瘤sLex(sialyl lewis X)表达及其与大肠癌临床病理参数的关系。方法应用免疫组织化学方法对55例大肠腺癌、15例远切端大肠黏膜及20例大肠腺瘤进行sLex检测。结果大肠腺瘤、大肠腺癌sLex阳性表达率分别为50.0%(9/18),80.0%...目的探讨大肠肿瘤sLex(sialyl lewis X)表达及其与大肠癌临床病理参数的关系。方法应用免疫组织化学方法对55例大肠腺癌、15例远切端大肠黏膜及20例大肠腺瘤进行sLex检测。结果大肠腺瘤、大肠腺癌sLex阳性表达率分别为50.0%(9/18),80.0%(44/55)(P=0.005);34例大肠腺癌、相应的癌旁正常黏膜及相应远切端正常黏膜sLex阳性表达率分别为79.4%(27/34),41.2%(14/34),5.88%(2/34)(P<0.05);55例大肠腺癌sLex阳性表达与患者的性别、部位、浸润深度、淋巴结转移、Duke's分期及生存时间无关(P>0.05),而与分化程度有关(P=0.021)。结论 sLex是检测大肠肿瘤发生及大肠癌分化程度的重要生物学标志物。展开更多
文摘Objective: To study the significance of E-selectin andits ligand-sLeX in the metastasis of hepatocellularcarcinoma (HCC).Methods: Flow cytometry and immunohistochemistrywere used to detect the expression of E-selectin and itsligand-sLeX in both HCC cell lines and human HCCtissues.Results: The positive rate of E-selectin in vascular en-dothelial cells adjacent to cancer was 67.9% (19/28). Theexpression of E-selectin in tumors accompanied withemboli or satellite foci was significantly higher than thatin tumors without emboli or satellite foci (P<0.05),and it was not related to tumor size, tumor capsule,AFP content, and the degree of differentiation. Thepositive expression of sLeX in SMMU-7721, PLF/PRF/5 and HepGⅡ cell lines was 7.03%, 63.35% and97.29% respectively. The positive cells of sLeX mainlydistributed in the margin of tumor tissues. The positiveexpression of sLeX in HCC cells in emboli or invasivetumor tissues was much higher than in primary foci.Conclusion: E-selectin and its ligand-sLeX are closelycorrelated with the metastasis of HCC.
文摘目的探讨大肠肿瘤sLex(sialyl lewis X)表达及其与大肠癌临床病理参数的关系。方法应用免疫组织化学方法对55例大肠腺癌、15例远切端大肠黏膜及20例大肠腺瘤进行sLex检测。结果大肠腺瘤、大肠腺癌sLex阳性表达率分别为50.0%(9/18),80.0%(44/55)(P=0.005);34例大肠腺癌、相应的癌旁正常黏膜及相应远切端正常黏膜sLex阳性表达率分别为79.4%(27/34),41.2%(14/34),5.88%(2/34)(P<0.05);55例大肠腺癌sLex阳性表达与患者的性别、部位、浸润深度、淋巴结转移、Duke's分期及生存时间无关(P>0.05),而与分化程度有关(P=0.021)。结论 sLex是检测大肠肿瘤发生及大肠癌分化程度的重要生物学标志物。
