A model for topographic correction and land surface reflectance estimation for optical remote sensing data in rugged terrian is presented.Considering a directional-directional reflectance that is used for direct solar...A model for topographic correction and land surface reflectance estimation for optical remote sensing data in rugged terrian is presented.Considering a directional-directional reflectance that is used for direct solar irradiance correction and a hemispheric-directional reflectance that is used for atmospheric diffuse irradiance and terrain background reflected irradiance correction respectively,the directional reflectance-based model for topographic effects removing and land surface reflectance calculation is developed by deducing the directional reflectance with topographic effects and using a radiative transfer model.A canopy reflectance simulated by GOMS model and Landsat/TM raw data covering Jiangxi rugged area were taken to validate the performance of the model presented in the paper.The validation results show that the model presented here has a remarkable ability to correct topography and estimate land surface reflectance and also provides a technique method for sequently quantitative remote sensing application in terrain area.展开更多
Our analyses of the monthly mean air temperature of meteorological stations show that altitude, global solar radiation and surface effective radiation have a significant impact on air temperature. We set up a physical...Our analyses of the monthly mean air temperature of meteorological stations show that altitude, global solar radiation and surface effective radiation have a significant impact on air temperature. We set up a physically-based empirical model for monthly air temperature simulation. Combined the proposed model with the distributed modeling results of global solar radiation and routine meteorological observation data, we also developed a method for the distributed simulation of monthly air temperatures over rugged terrain. Spatial distribution maps are generated at a resolution of 1 km×1 km for the monthly mean, the monthly mean maximum and the monthly mean minimum air temperatures for the Yellow River Basin. Analysis shows that the simulation results reflect to a considerable extent the macro and local distribution characteristics of air temperature. Cross-validation shows that the proposed model displays good stability with mean absolute bias errors of 0.19°C–0.35°C. Tests carried out on local meteorological station data and case year data show that the model has good spatial and temporal simulation capacity. The proposed model solely uses routine meteorological data and can be applied easily to other regions.展开更多
A simple, environmentally friendly and high efficient extraction method was proposed for the determina- tion of five phthalates in aquatic products by gas chromatography combined with mass spectrometry detector （GC-M...A simple, environmentally friendly and high efficient extraction method was proposed for the determina- tion of five phthalates in aquatic products by gas chromatography combined with mass spectrometry detector （GC-MS）. When this method was adopted, samples were pretreated by modified QuEChERS（quick, easy, cheap, effective, rugged and safe） method. An environmentally friendly extractant（ethanol aqueous solution） replaced toxic acetonitrile for extracting phthalates in the protein-matrix samples. Fluorescence quenching spectra of bovine serum albumin（BSA） with phthalates show that there was a high-affinity interaction between phthalate and protein, de- creasing the extraction efficiency of phthalates from fish samples. However, in the 80%（volume fraction, ~） ethanol aqueous solution, a slow but full protein denaturation takes place, which would cause the unfolding of protein and the release of phthalates. Meanwhile, the fat-soluble impurities are extracted less in ^（ethanol） 80% aqueous solution than in either ~p（ethanol） 100% or hydrophobic solvents in the liquid-liquid extraction procedure. Therefore the puri- fication steps were greatly simplified. Overall recoveries were 81.77%--90.5% with limits of detection between 2.53 and 9.61 ~tg/L, and relative standard deviation values at 1.15%---4.85%. The proposed approach was satisfactorily applied to the determination of phthalates in real aquatic products, such as fish, shrimp and oyster.展开更多
We present a method based on least-squares reverse time migration with plane-wave encoding （P-LSRTM） for rugged topography. Instead of modifying the wave field before migration, we modify the plane-wave encoding fun...We present a method based on least-squares reverse time migration with plane-wave encoding （P-LSRTM） for rugged topography. Instead of modifying the wave field before migration, we modify the plane-wave encoding function and fill constant velocity to the area above rugged topography in the model so that P-LSRTM can be directly performed from rugged surface in the way same to shot domain reverse time migration. In order to improve efficiency and reduce I/O （input/output） cost, the dynamic en- coding strategy and hybrid encoding strategy are implemented. Numerical test on SEG rugged topography model show that P-LSRTM can suppress migration artifacts in the migration image, and compensate am- plitude in the middle-deep part efficiently. Without data correction, P-LSRTM can produce a satisfying image of near-surface if we could get an accurate near-surface velocity model. Moreover, the pre-stack P- LSRTM is more robust than conventional RTM in the presence of migration velocity errors.展开更多
Global solar radiation（GSR） is the most direct source and form of global energy, and calculation of its quantity is highly complex due to influences of local topography and terrain inter-shielding. Digital elevation...Global solar radiation（GSR） is the most direct source and form of global energy, and calculation of its quantity is highly complex due to influences of local topography and terrain inter-shielding. Digital elevation model（DEM） data as a representation of the complex terrain and multiplicity condition produces a series of topographic factors（e.g. slope, aspect, etc.）. Based on 1 km resolution DEM data, meteorological observations and NOAA-AVHRR remote sensing data, a distributed model for the calculation of GSR over rugged terrain within the Yangtze River Basin has been developed. The overarching model permits calculation of astronomical solar radiation for rugged topography and comprises a distributed direct solar radiation model, a distributed diffuse radiation model and a distributed terrain reflectance radiation model. Using the developed model, a quantitative simulation of the GSR space distribution and visualization has been undertaken, with results subsequently analyzed with respect to locality and terrain. Analyses suggest that GSR magnitude is seasonally affected, while the degree of influence was found to increase in concurrence with increasing altitude. Moreover, GSR magnitude exhibited clear spatial variation with respect to the dominant local aspect; GSR values associated with the sunny southern slopes were significantly greater than those associated with shaded slopes. Error analysis indicates a mean absolute error of 12.983 MJm-2 and a mean relative error of 3.608%, while the results based on a site authentication procedure display an absolute error of 22.621 MJm-2 and a relative error of 4.626%.展开更多
Due to the influences of local topographical factors and terrain inter-shielding, calculation of direct solar radiation （DSR） quantity of rugged terrain is very complex. Based on digital elevation model （DEM） data...Due to the influences of local topographical factors and terrain inter-shielding, calculation of direct solar radiation （DSR） quantity of rugged terrain is very complex. Based on digital elevation model （DEM） data and meteorological observations, a distributed model for calculating DSR over rugged terrain is developed. This model gives an all-sided consideration on factors influencing th a resolution of 1 km × 1 km for thDSR. Using the developed model, normals of annual DSR quantity wie Yellow River Basin was generated, with DEM data as the general characterization of terrain. Characteristics of DSR quantity influenced by geographic and topographic factors over rugged terrain were analyzed thoroughly. Results suggest that： influenced by local topographic factors, i.e. azimuth, slope and so on, and annual DSR quantity over mountainous area has a clear spatial difference; annual DSR quantity of sunny slope （or southern slope） of mountains is obviously larger than that of shady slope （or northern slope）. The calculated DSR quantity of the Yellow River Basin is provided in the same way as other kinds of spatial information and can be employed as basic geographic data for relevant studies as well.展开更多
Based on the developed distributed model for calculating astronomical solar radiation (ASR), monthly ASR with a resolution of 1 km×1 km for the rugged terrains of Yellow River Basin was calculated, with DEM data ...Based on the developed distributed model for calculating astronomical solar radiation (ASR), monthly ASR with a resolution of 1 km×1 km for the rugged terrains of Yellow River Basin was calculated, with DEM data as the general characterization of terrain. This model gives an all-sided consideration on factors that influence the ASR. Results suggest that (1) Annual ASR has a progressive decrease trend from south to north; (2) the magnitude order of seasonal ASR is: summer>spring>autumn>winter; (3) topographical factors have robust effect on the spatial distribution of ASR, particularly in winter when a lower sun elevation angle exists; (4) the ASR of slopes with a sunny exposure is generally 2 or 3 times that of slopes with a shading exposure and the extreme difference of ASR for different terrains is over 10 times in January; (5) the spatial differences of ASR are relatively small in summer when a higher sun elevation angle exists and the extreme difference of ASR for different terrains is only 16% in July; and (6) the sequence of topographical influence strength is: winter>autumn>spring>summer.展开更多
目的以丹参为研究对象,比较2020年版《中华人民共和国药典》中3类前处理方法对33种禁用农药残留物回收率的影响,进一步优化快速样品处理法(quick,easy,cheap,effective,rugged and safe,QuEChERS)。方法分别从基质效应和回收率方面比较...目的以丹参为研究对象,比较2020年版《中华人民共和国药典》中3类前处理方法对33种禁用农药残留物回收率的影响,进一步优化快速样品处理法(quick,easy,cheap,effective,rugged and safe,QuEChERS)。方法分别从基质效应和回收率方面比较《中华人民共和国药典》3类前处理方法,并对QuEChERS法进行研究与优化,样品加水后由1%醋酸-乙腈提取,依次加入QuEChERS盐包与QuEChERS净化管盐析净化,提取液经浓缩后分别加入水和内标磷酸三苯酯,结合液相色谱-串联质谱法和气相色谱-串联质谱法以添加水平为定量限浓度水平测定样品的回收率和RSD。结果采用《中华人民共和国药典》前处理方法时,丹参中33种禁用农药(54个单体)在添加水平为定量限浓度水平时,部分农药回收率较低;优化QuEChERS法后,丹参中33种禁用农药(54个单体)在一定浓度下,线性关系良好,r均大于0.990,其回收率为64.43%~127.35%时,RSD均<15%;199批饮片和60批药材中共检出2种农药,甲拌磷检出率为3.86%,克百威检出率为1.93%,但残留量均未超过2020年版《中华人民共和国药典》规定限度。结论优化后的QuEChERS法快速简便,有针对性地对丹参中33种禁用农药残留进行准确检测,对于丹参的生产种植与日常监控具有一定的参考意义。展开更多
Background:The grazing behaviour of herbivores and their grazing personalities might in part be determined genetically,but there are few studies in beef cattle illustrating this.In this study,we investigated for first...Background:The grazing behaviour of herbivores and their grazing personalities might in part be determined genetically,but there are few studies in beef cattle illustrating this.In this study,we investigated for first time the genetic variation within a candidate‘grazing gene',the glutamate metabotropic receptor 5 gene(GRM5),and tested associations between variation in that gene and variation in grazing personality behaviours(GP-behaviours)displayed by free-ranging cows during winter grazing in the steep and rugged rangelands of New Zealand.Mature beef cows(n=303,from 3 to 10 years of age)were tracked with global positioning system(GPS)and,with 5-minutes(min)relocation frequency,various GP-behaviours were calculated.These included horizontal and vertical distances travelled,mean elevation,elevation range,elevation gain,slope,home range and movement tortuosity,variously calculated using daily relocation trajectories with repeated measurements(i.e.,7 to 24 days(d))and satellite-derived digital elevation models(DEM).The different GP-behaviours were fitted into mixed models to ascertain their associations with variant sequences and genotypes of GRM5.Results:We discovered three GRM5 variants(A,B and C)and identified the six possible genotypes in the cattle studied.The mixed models revealed that A was significantly associated with elevation range,home range and movement tortuosity.Similarly,GRM5 genotypes were associated(P<0.05)to home range and movement tortuosity,while trends suggesting association(P<0.1)were also revealed for elevation range and horizontal distance travelled.Most GP-behaviour models were improved by correcting for cow age-class as a fixed factor.The analysis of GP-behaviours averaged per cow age-class suggests that grazing personality is fully established as beef cows reached 4 years of age.Home range and movement tortuosity were not only associated with GRM5 variation,but also negatively correlated with each other(r=-0.27,P<0.001).Conclusions:There seems to be a genetically determined trade-off b展开更多
采用串联固相萃取法(Extrelut-PRS-C18固相萃取柱萃取法、PRS-C18固相萃取柱萃取法)、MCX固相萃取柱萃取法和分散固相萃取法(QuEChERS(quick, easy, cheap, effective, rugged, and safe)法)4种前处理方法对肉制品中16种杂环胺(heterocy...采用串联固相萃取法(Extrelut-PRS-C18固相萃取柱萃取法、PRS-C18固相萃取柱萃取法)、MCX固相萃取柱萃取法和分散固相萃取法(QuEChERS(quick, easy, cheap, effective, rugged, and safe)法)4种前处理方法对肉制品中16种杂环胺(heterocyclic amines,HAs)进行提取,并从方法学和提取成本角度综合比较4种方法。结果表明:4种提取方法得到的16种HAs的回收率和精密度及检出限(limit of detection,LOD)和定量限(limit of quantification,LOQ)存在显著差异,其中QuEChERS法回收率和精密度均优于其他3种方法。MCX固相萃取柱萃取法和QuEChERS法的LOD和LOQ优于其他2种方法;MCX固相萃取柱萃取法和QuEChERS法所需经济和时间成本较低。综合分析,QuEChERS法具有快速、简便、便宜、有效、稳固和安全的特点,有利于肉制品中HAs的准确检测,并值得推广应用。展开更多
A method for constructing core-germplasm of Rosa rugosa in China based on molecular marker data was probed and the optimum core germplasm was established. Studies were initiated to analyze the genetic diversity of 120...A method for constructing core-germplasm of Rosa rugosa in China based on molecular marker data was probed and the optimum core germplasm was established. Studies were initiated to analyze the genetic diversity of 120 rugged roses from 6 different Chinese source populations based on CDDP marker, and a preliminary construction of the core collection was established using stepwise UPGMA clustering sampling method. The 26 core collection resources of R. rugosa collections in China have 20% germplasm samples of initial collection, the retention ratio of polymorphic loci, effective number of alleles (Ne), Nei’s genetic diversity (H) and Shannon information index (I) were respectively 97.52%, 104.16%, 108.38% and 106.18%. The results of t-test showed that no significant difference was found in genetic diversity indexes between the core collection and the original collection. These results also demonstrated that the core collection could stand for original collection excellently. The results show that CDDP molecular technology can be successfully applied to the construction of core germplasm resources of rugged roses.展开更多
基金the National Natural Science Foundation of China (Grant No.40730525)Knowledge Innovation Engineering Project of Chinese Academy of Sciences (Grant No.KZCX2-YW-313)China's Special Funds for Major State Basic Research Project (Grant No.2007CB714401)
文摘A model for topographic correction and land surface reflectance estimation for optical remote sensing data in rugged terrian is presented.Considering a directional-directional reflectance that is used for direct solar irradiance correction and a hemispheric-directional reflectance that is used for atmospheric diffuse irradiance and terrain background reflected irradiance correction respectively,the directional reflectance-based model for topographic effects removing and land surface reflectance calculation is developed by deducing the directional reflectance with topographic effects and using a radiative transfer model.A canopy reflectance simulated by GOMS model and Landsat/TM raw data covering Jiangxi rugged area were taken to validate the performance of the model presented in the paper.The validation results show that the model presented here has a remarkable ability to correct topography and estimate land surface reflectance and also provides a technique method for sequently quantitative remote sensing application in terrain area.
基金Supported by China Meteorological Administration key Project on New Technique Diffusion (Grant No. CMATG2006Z10)Jiangsu Key Laboratory of Meteoro-logical Disasters (Grant No. KLME050102)
文摘Our analyses of the monthly mean air temperature of meteorological stations show that altitude, global solar radiation and surface effective radiation have a significant impact on air temperature. We set up a physically-based empirical model for monthly air temperature simulation. Combined the proposed model with the distributed modeling results of global solar radiation and routine meteorological observation data, we also developed a method for the distributed simulation of monthly air temperatures over rugged terrain. Spatial distribution maps are generated at a resolution of 1 km×1 km for the monthly mean, the monthly mean maximum and the monthly mean minimum air temperatures for the Yellow River Basin. Analysis shows that the simulation results reflect to a considerable extent the macro and local distribution characteristics of air temperature. Cross-validation shows that the proposed model displays good stability with mean absolute bias errors of 0.19°C–0.35°C. Tests carried out on local meteorological station data and case year data show that the model has good spatial and temporal simulation capacity. The proposed model solely uses routine meteorological data and can be applied easily to other regions.
基金Supported by the National Natural Science Foundation of China（No.20973149） and the Natural Science Foundation of Shandong Province, China（No.ZR2011BM009）.
文摘A simple, environmentally friendly and high efficient extraction method was proposed for the determina- tion of five phthalates in aquatic products by gas chromatography combined with mass spectrometry detector （GC-MS）. When this method was adopted, samples were pretreated by modified QuEChERS（quick, easy, cheap, effective, rugged and safe） method. An environmentally friendly extractant（ethanol aqueous solution） replaced toxic acetonitrile for extracting phthalates in the protein-matrix samples. Fluorescence quenching spectra of bovine serum albumin（BSA） with phthalates show that there was a high-affinity interaction between phthalate and protein, de- creasing the extraction efficiency of phthalates from fish samples. However, in the 80%（volume fraction, ~） ethanol aqueous solution, a slow but full protein denaturation takes place, which would cause the unfolding of protein and the release of phthalates. Meanwhile, the fat-soluble impurities are extracted less in ^（ethanol） 80% aqueous solution than in either ~p（ethanol） 100% or hydrophobic solvents in the liquid-liquid extraction procedure. Therefore the puri- fication steps were greatly simplified. Overall recoveries were 81.77%--90.5% with limits of detection between 2.53 and 9.61 ~tg/L, and relative standard deviation values at 1.15%---4.85%. The proposed approach was satisfactorily applied to the determination of phthalates in real aquatic products, such as fish, shrimp and oyster.
基金jointly financial support of the National 973 Project of China(Nos.2014CB239006,2011CB202402)the National Natural Science Foundation of China(Nos.41104069,41274124)+1 种基金the Shandong Natural Science Foundation of China(No.ZR2011DQ016)the Fundamental Research Funds for the Central Universities of China(No.R1401005A)
文摘We present a method based on least-squares reverse time migration with plane-wave encoding （P-LSRTM） for rugged topography. Instead of modifying the wave field before migration, we modify the plane-wave encoding function and fill constant velocity to the area above rugged topography in the model so that P-LSRTM can be directly performed from rugged surface in the way same to shot domain reverse time migration. In order to improve efficiency and reduce I/O （input/output） cost, the dynamic en- coding strategy and hybrid encoding strategy are implemented. Numerical test on SEG rugged topography model show that P-LSRTM can suppress migration artifacts in the migration image, and compensate am- plitude in the middle-deep part efficiently. Without data correction, P-LSRTM can produce a satisfying image of near-surface if we could get an accurate near-surface velocity model. Moreover, the pre-stack P- LSRTM is more robust than conventional RTM in the presence of migration velocity errors.
基金National Natural Science Foundation of China,No.41175077 National Natural Science Foundation for Young Scholars,No.S0508016001 Guizhou Branch Major Projects[2011],No.6003
文摘Global solar radiation（GSR） is the most direct source and form of global energy, and calculation of its quantity is highly complex due to influences of local topography and terrain inter-shielding. Digital elevation model（DEM） data as a representation of the complex terrain and multiplicity condition produces a series of topographic factors（e.g. slope, aspect, etc.）. Based on 1 km resolution DEM data, meteorological observations and NOAA-AVHRR remote sensing data, a distributed model for the calculation of GSR over rugged terrain within the Yangtze River Basin has been developed. The overarching model permits calculation of astronomical solar radiation for rugged topography and comprises a distributed direct solar radiation model, a distributed diffuse radiation model and a distributed terrain reflectance radiation model. Using the developed model, a quantitative simulation of the GSR space distribution and visualization has been undertaken, with results subsequently analyzed with respect to locality and terrain. Analyses suggest that GSR magnitude is seasonally affected, while the degree of influence was found to increase in concurrence with increasing altitude. Moreover, GSR magnitude exhibited clear spatial variation with respect to the dominant local aspect; GSR values associated with the sunny southern slopes were significantly greater than those associated with shaded slopes. Error analysis indicates a mean absolute error of 12.983 MJm-2 and a mean relative error of 3.608%, while the results based on a site authentication procedure display an absolute error of 22.621 MJm-2 and a relative error of 4.626%.
文摘Due to the influences of local topographical factors and terrain inter-shielding, calculation of direct solar radiation （DSR） quantity of rugged terrain is very complex. Based on digital elevation model （DEM） data and meteorological observations, a distributed model for calculating DSR over rugged terrain is developed. This model gives an all-sided consideration on factors influencing th a resolution of 1 km × 1 km for thDSR. Using the developed model, normals of annual DSR quantity wie Yellow River Basin was generated, with DEM data as the general characterization of terrain. Characteristics of DSR quantity influenced by geographic and topographic factors over rugged terrain were analyzed thoroughly. Results suggest that： influenced by local topographic factors, i.e. azimuth, slope and so on, and annual DSR quantity over mountainous area has a clear spatial difference; annual DSR quantity of sunny slope （or southern slope） of mountains is obviously larger than that of shady slope （or northern slope）. The calculated DSR quantity of the Yellow River Basin is provided in the same way as other kinds of spatial information and can be employed as basic geographic data for relevant studies as well.
基金Major State Basic Research Development Program of ChinaNo.G19990436-01 No.G20000779
文摘Based on the developed distributed model for calculating astronomical solar radiation (ASR), monthly ASR with a resolution of 1 km×1 km for the rugged terrains of Yellow River Basin was calculated, with DEM data as the general characterization of terrain. This model gives an all-sided consideration on factors that influence the ASR. Results suggest that (1) Annual ASR has a progressive decrease trend from south to north; (2) the magnitude order of seasonal ASR is: summer>spring>autumn>winter; (3) topographical factors have robust effect on the spatial distribution of ASR, particularly in winter when a lower sun elevation angle exists; (4) the ASR of slopes with a sunny exposure is generally 2 or 3 times that of slopes with a shading exposure and the extreme difference of ASR for different terrains is over 10 times in January; (5) the spatial differences of ASR are relatively small in summer when a higher sun elevation angle exists and the extreme difference of ASR for different terrains is only 16% in July; and (6) the sequence of topographical influence strength is: winter>autumn>spring>summer.
文摘目的以丹参为研究对象,比较2020年版《中华人民共和国药典》中3类前处理方法对33种禁用农药残留物回收率的影响,进一步优化快速样品处理法(quick,easy,cheap,effective,rugged and safe,QuEChERS)。方法分别从基质效应和回收率方面比较《中华人民共和国药典》3类前处理方法,并对QuEChERS法进行研究与优化,样品加水后由1%醋酸-乙腈提取,依次加入QuEChERS盐包与QuEChERS净化管盐析净化,提取液经浓缩后分别加入水和内标磷酸三苯酯,结合液相色谱-串联质谱法和气相色谱-串联质谱法以添加水平为定量限浓度水平测定样品的回收率和RSD。结果采用《中华人民共和国药典》前处理方法时,丹参中33种禁用农药(54个单体)在添加水平为定量限浓度水平时,部分农药回收率较低;优化QuEChERS法后,丹参中33种禁用农药(54个单体)在一定浓度下,线性关系良好,r均大于0.990,其回收率为64.43%~127.35%时,RSD均<15%;199批饮片和60批药材中共检出2种农药,甲拌磷检出率为3.86%,克百威检出率为1.93%,但残留量均未超过2020年版《中华人民共和国药典》规定限度。结论优化后的QuEChERS法快速简便,有针对性地对丹参中33种禁用农药残留进行准确检测,对于丹参的生产种植与日常监控具有一定的参考意义。
基金CAMG was funded with a Lincoln University Ph D scholarship(Lincoln University Centennial Trust,New Zealand)with research funding from the New Zealand Hereford Association(NZHA)the Hellaby Grasslands Trust。
文摘Background:The grazing behaviour of herbivores and their grazing personalities might in part be determined genetically,but there are few studies in beef cattle illustrating this.In this study,we investigated for first time the genetic variation within a candidate‘grazing gene',the glutamate metabotropic receptor 5 gene(GRM5),and tested associations between variation in that gene and variation in grazing personality behaviours(GP-behaviours)displayed by free-ranging cows during winter grazing in the steep and rugged rangelands of New Zealand.Mature beef cows(n=303,from 3 to 10 years of age)were tracked with global positioning system(GPS)and,with 5-minutes(min)relocation frequency,various GP-behaviours were calculated.These included horizontal and vertical distances travelled,mean elevation,elevation range,elevation gain,slope,home range and movement tortuosity,variously calculated using daily relocation trajectories with repeated measurements(i.e.,7 to 24 days(d))and satellite-derived digital elevation models(DEM).The different GP-behaviours were fitted into mixed models to ascertain their associations with variant sequences and genotypes of GRM5.Results:We discovered three GRM5 variants(A,B and C)and identified the six possible genotypes in the cattle studied.The mixed models revealed that A was significantly associated with elevation range,home range and movement tortuosity.Similarly,GRM5 genotypes were associated(P<0.05)to home range and movement tortuosity,while trends suggesting association(P<0.1)were also revealed for elevation range and horizontal distance travelled.Most GP-behaviour models were improved by correcting for cow age-class as a fixed factor.The analysis of GP-behaviours averaged per cow age-class suggests that grazing personality is fully established as beef cows reached 4 years of age.Home range and movement tortuosity were not only associated with GRM5 variation,but also negatively correlated with each other(r=-0.27,P<0.001).Conclusions:There seems to be a genetically determined trade-off b
文摘采用串联固相萃取法(Extrelut-PRS-C18固相萃取柱萃取法、PRS-C18固相萃取柱萃取法)、MCX固相萃取柱萃取法和分散固相萃取法(QuEChERS(quick, easy, cheap, effective, rugged, and safe)法)4种前处理方法对肉制品中16种杂环胺(heterocyclic amines,HAs)进行提取,并从方法学和提取成本角度综合比较4种方法。结果表明:4种提取方法得到的16种HAs的回收率和精密度及检出限(limit of detection,LOD)和定量限(limit of quantification,LOQ)存在显著差异,其中QuEChERS法回收率和精密度均优于其他3种方法。MCX固相萃取柱萃取法和QuEChERS法的LOD和LOQ优于其他2种方法;MCX固相萃取柱萃取法和QuEChERS法所需经济和时间成本较低。综合分析,QuEChERS法具有快速、简便、便宜、有效、稳固和安全的特点,有利于肉制品中HAs的准确检测,并值得推广应用。
文摘A method for constructing core-germplasm of Rosa rugosa in China based on molecular marker data was probed and the optimum core germplasm was established. Studies were initiated to analyze the genetic diversity of 120 rugged roses from 6 different Chinese source populations based on CDDP marker, and a preliminary construction of the core collection was established using stepwise UPGMA clustering sampling method. The 26 core collection resources of R. rugosa collections in China have 20% germplasm samples of initial collection, the retention ratio of polymorphic loci, effective number of alleles (Ne), Nei’s genetic diversity (H) and Shannon information index (I) were respectively 97.52%, 104.16%, 108.38% and 106.18%. The results of t-test showed that no significant difference was found in genetic diversity indexes between the core collection and the original collection. These results also demonstrated that the core collection could stand for original collection excellently. The results show that CDDP molecular technology can be successfully applied to the construction of core germplasm resources of rugged roses.
