为明确辽宁地区韭菜田根蛆发生种类及优势种的消长动态,于2014-2017年分别采用黄色水盆诱集和挖根的方法对根蛆成虫和幼虫的发生和为害规律进行了系统调查研究。结果表明,辽宁韭菜田根蛆主要有韭菜迟眼蕈蚊Bradysia odoriphaga Yang et ...为明确辽宁地区韭菜田根蛆发生种类及优势种的消长动态,于2014-2017年分别采用黄色水盆诱集和挖根的方法对根蛆成虫和幼虫的发生和为害规律进行了系统调查研究。结果表明,辽宁韭菜田根蛆主要有韭菜迟眼蕈蚊Bradysia odoriphaga Yang et Zhang和食用菌异迟眼蕈蚊Bradysia difformis Frey,二者常常混合发生。北镇地区优势种为韭菜迟眼蕈蚊,年发生7~8代,其中冬季温室期发生3~4代。初冬扣棚后根蛆为害期与冬韭生产期高度重合。沈阳东陵地区优势种为食用菌异迟眼蕈蚊,其在露地韭菜上年发生5代。根蛆在田间呈聚集分布,聚集程度随种群密度的增大而升高。施用有机肥可显著降低根蛆的为害程度。根蛆以3龄幼虫在大葱上越冬。辽宁地区根蛆的发生规律呈现冬季发生重,春、秋季略轻发生,夏季基本不发生的特点。展开更多
Sugar beet root maggot （SBRM, Tetanops myopaeformis von R6der） is a major but poorly understood insect pest of sugar beet （Beta vulgaris L.）. The molecular mecha- nisms underlying plant defense responses are well ...Sugar beet root maggot （SBRM, Tetanops myopaeformis von R6der） is a major but poorly understood insect pest of sugar beet （Beta vulgaris L.）. The molecular mecha- nisms underlying plant defense responses are well documented, however, little information is available about complementary mechanisms for insect adaptive responses to overcome host resistance. To date, no studies have been published on SBRM gene expression pro- filing. Suppressive subtractive hybridization （SSH） generated more than 300 SBRM ESTs differentially expressed in the interaction of the pest with a moderately resistant （F1016） and a susceptible （F1010） sugar beet line. Blast2GO v. 3.2 search indicated that over 40% of the differentially expressed genes had known functions, primarily driven by fruit fly D. melanogaster genes. Expression patterns of 18 selected EST clones were confirmed by RT-PCR analysis. Gene Ontology （GO） analysis predicted a dominance of metabolic and catalytic genes involved in the interaction of SBRM with its host. SBRM genes function- ing during development, regulation, cellular process, signaling and under stress conditions were annotated. SBRM genes that were common or unique in response to resistant or susceptible interactions with the host were identified and their possible roles in insect responses to the host are discussed.展开更多
文摘为明确辽宁地区韭菜田根蛆发生种类及优势种的消长动态,于2014-2017年分别采用黄色水盆诱集和挖根的方法对根蛆成虫和幼虫的发生和为害规律进行了系统调查研究。结果表明,辽宁韭菜田根蛆主要有韭菜迟眼蕈蚊Bradysia odoriphaga Yang et Zhang和食用菌异迟眼蕈蚊Bradysia difformis Frey,二者常常混合发生。北镇地区优势种为韭菜迟眼蕈蚊,年发生7~8代,其中冬季温室期发生3~4代。初冬扣棚后根蛆为害期与冬韭生产期高度重合。沈阳东陵地区优势种为食用菌异迟眼蕈蚊,其在露地韭菜上年发生5代。根蛆在田间呈聚集分布,聚集程度随种群密度的增大而升高。施用有机肥可显著降低根蛆的为害程度。根蛆以3龄幼虫在大葱上越冬。辽宁地区根蛆的发生规律呈现冬季发生重,春、秋季略轻发生,夏季基本不发生的特点。
文摘Sugar beet root maggot （SBRM, Tetanops myopaeformis von R6der） is a major but poorly understood insect pest of sugar beet （Beta vulgaris L.）. The molecular mecha- nisms underlying plant defense responses are well documented, however, little information is available about complementary mechanisms for insect adaptive responses to overcome host resistance. To date, no studies have been published on SBRM gene expression pro- filing. Suppressive subtractive hybridization （SSH） generated more than 300 SBRM ESTs differentially expressed in the interaction of the pest with a moderately resistant （F1016） and a susceptible （F1010） sugar beet line. Blast2GO v. 3.2 search indicated that over 40% of the differentially expressed genes had known functions, primarily driven by fruit fly D. melanogaster genes. Expression patterns of 18 selected EST clones were confirmed by RT-PCR analysis. Gene Ontology （GO） analysis predicted a dominance of metabolic and catalytic genes involved in the interaction of SBRM with its host. SBRM genes function- ing during development, regulation, cellular process, signaling and under stress conditions were annotated. SBRM genes that were common or unique in response to resistant or susceptible interactions with the host were identified and their possible roles in insect responses to the host are discussed.
