Objective To investigate the genotypic diversity of Methicillin-resistant Staphylococcus aureus （MRSA） isolated from pigs and retail foods from different geographical areas in China and further to study the routes a...Objective To investigate the genotypic diversity of Methicillin-resistant Staphylococcus aureus （MRSA） isolated from pigs and retail foods from different geographical areas in China and further to study the routes and rates of transmission of this pathogen from animals to food. Methods Seventy-one MRSA isolates were obtained from pigs and retail foods and then characterized by multi-locus sequencing typing （MLST）, spa typing, multiple-locus variable number of tandem repeat analysis （MLVA）, pulsed-field gel electrophoresis （PFGE）, and antimicrobial susceptibility testing. Results All isolated MRSA exhibited multi-drug resistance （MDR）. Greater diversity was found in food-associated MRSA （7 STs, 8 spa types, and 10 MLVA patterns） compared to pig-associated MRSA （3 STs, 1 spa type, and 6 MLVA patterns）. PFGE patterns were more diverse for pig-associated MRSA than those of food-associated isolates （40 vs. 11 pulse types）. Among the pig-associated isolates, CC9-ST9-t899-MC2236 was the most prevalent clone （96.4%）, and CC9-ST9-t437-MC621 （20.0%） was the predominant clone among the food-associated isolates. The CC9-ST9 isolates showed significantly higher antimicrobial resistance than other clones. Interestingly, CC398-ST398-t034 clone was identified from both pig- and food-associated isolates. Of note, some community- and hospital-associated MRSA strains （t030, t172, t1244, and t4549） were also identified as food-associated isolates. Conclusion CC9-ST9-t899-MC2236-MDR was the most predominant clone in pigs, but significant genetic diversity was observed in food-associated MRSA. Our results demonstrate the great need for improved surveillance of MRSA in livestock and food and effective prevention strategies to limit MDR-MRSA infections in China.展开更多
In this paper, we deal with a purchase problem for food retailing, and formulate a two-level linear programming problem with a food retailer and a distributer. The food retailer deals with vegetables and fruits which ...In this paper, we deal with a purchase problem for food retailing, and formulate a two-level linear programming problem with a food retailer and a distributer. The food retailer deals with vegetables and fruits which are purchased from the distributer;the distributer buys vegetables and fruits ordered from the food retailer at the central wholesale markets in several cities, and transports them by truck from each of the central wholesaler markets to the food retailer’s storehouse. We solve the two-level linear programming problem in which the profits of the food retailer and the distributer are maximized.展开更多
In Italy, and particularly in its southern area, super-markets and hyper-markets still represent an expanding retailing format, as they were introduced years later than in other western countries. Today, they have bec...In Italy, and particularly in its southern area, super-markets and hyper-markets still represent an expanding retailing format, as they were introduced years later than in other western countries. Today, they have become large retailers capable of influencing local development from an economic, social and urban perspective. The literature concerned with their effects on the local economy has focused on labour markets, price dynamics, and inter-type competition, neglecting their impact on macroeconomic factors such as GDP, value added specific sectors--agriculture, industry and services. This study tries to contribute to fill this gap by empirically investigating associations between key characteristics of these retailers and specific macroeconomic value added factors展开更多
基金funded by the National Key R&D Program of China(2016YFD0401102)(the National Key Research and Development Program of China,the Ministry of Science and Technology of China)China Food Safety Talent Competency Development Initiative:CFSA 523 Program(1311613106702)
文摘Objective To investigate the genotypic diversity of Methicillin-resistant Staphylococcus aureus （MRSA） isolated from pigs and retail foods from different geographical areas in China and further to study the routes and rates of transmission of this pathogen from animals to food. Methods Seventy-one MRSA isolates were obtained from pigs and retail foods and then characterized by multi-locus sequencing typing （MLST）, spa typing, multiple-locus variable number of tandem repeat analysis （MLVA）, pulsed-field gel electrophoresis （PFGE）, and antimicrobial susceptibility testing. Results All isolated MRSA exhibited multi-drug resistance （MDR）. Greater diversity was found in food-associated MRSA （7 STs, 8 spa types, and 10 MLVA patterns） compared to pig-associated MRSA （3 STs, 1 spa type, and 6 MLVA patterns）. PFGE patterns were more diverse for pig-associated MRSA than those of food-associated isolates （40 vs. 11 pulse types）. Among the pig-associated isolates, CC9-ST9-t899-MC2236 was the most prevalent clone （96.4%）, and CC9-ST9-t437-MC621 （20.0%） was the predominant clone among the food-associated isolates. The CC9-ST9 isolates showed significantly higher antimicrobial resistance than other clones. Interestingly, CC398-ST398-t034 clone was identified from both pig- and food-associated isolates. Of note, some community- and hospital-associated MRSA strains （t030, t172, t1244, and t4549） were also identified as food-associated isolates. Conclusion CC9-ST9-t899-MC2236-MDR was the most predominant clone in pigs, but significant genetic diversity was observed in food-associated MRSA. Our results demonstrate the great need for improved surveillance of MRSA in livestock and food and effective prevention strategies to limit MDR-MRSA infections in China.
文摘In this paper, we deal with a purchase problem for food retailing, and formulate a two-level linear programming problem with a food retailer and a distributer. The food retailer deals with vegetables and fruits which are purchased from the distributer;the distributer buys vegetables and fruits ordered from the food retailer at the central wholesale markets in several cities, and transports them by truck from each of the central wholesaler markets to the food retailer’s storehouse. We solve the two-level linear programming problem in which the profits of the food retailer and the distributer are maximized.
文摘In Italy, and particularly in its southern area, super-markets and hyper-markets still represent an expanding retailing format, as they were introduced years later than in other western countries. Today, they have become large retailers capable of influencing local development from an economic, social and urban perspective. The literature concerned with their effects on the local economy has focused on labour markets, price dynamics, and inter-type competition, neglecting their impact on macroeconomic factors such as GDP, value added specific sectors--agriculture, industry and services. This study tries to contribute to fill this gap by empirically investigating associations between key characteristics of these retailers and specific macroeconomic value added factors
