Nanostructured diamond have potential applications in many biomedical related fields and demonstrated extraordinary capacity to influence cellular responses. Studying the surface property of nanodiamond and its influe...Nanostructured diamond have potential applications in many biomedical related fields and demonstrated extraordinary capacity to influence cellular responses. Studying the surface property of nanodiamond and its influence to protein adsorption and subsequent cellular responses along with the mechanism behind such capacity becomes more important. Here the role of surface energy associated with nanostructured diamond in modulating fibronectin and osteoblast(OB, bone forming cells) responses was investigated. Nanocrystalline diamond(NCD) and submicron crystalline diamond(SMCD) films with controllable surface energy were prepared by microwave-enhanced plasma chemical vapor deposition(MPCVD) techniques. Fibronectin adsorption on the diamond films with varied surface energy values was measured via the enzyme-linked immunosorbent assay(ELISA) and the relationship between the surface energy and fibronectin adsorption was studied. The result indicated that fibronectin adsorption on nanostructured surfaces was closely related to both surface energy and material microstructures. The spreading and migration of OB aggregates(each containing 30–50 cells) on the NCD with varied surface energy values were also studied. The result indicates a correlation between the cell spreading and migration on nanodiamond and the surface energy of nanostructured surface.展开更多
Objective To investigate the pathogenesis and immunogenicity of H9N2 influenza virus A/Guangzhou/333/99 （a reassortant of G1 and G9 viruses isolated from a female patient in 1999） in a mouse model of infection.Metho...Objective To investigate the pathogenesis and immunogenicity of H9N2 influenza virus A/Guangzhou/333/99 （a reassortant of G1 and G9 viruses isolated from a female patient in 1999） in a mouse model of infection.Methods Mice were infected with increasing virus titers.Viral load in the lungs and trachea was determined by EID50 assay.Pulmonary histopathology was assessed by hematoxylin‐eosin staining.Anti‐HI antibody titers and T‐cell responses to viral HA were determined by ELISPOT and confirmed by flow cytometry.Results Mice presented a mild syndrome after intranasal infection with A/Guangzhou/333/99 （H9N2） influenza virus.Virus was detected in the trachea and lungs of mice harvested on days 3,6,and 9 post‐infection.A T‐cell response to viral HA was detected on day 6 and H9 HA‐specific CD 4＋ T‐cells predominated.Seroconversion was detected after 14 days and antibody persisted for at least 28 weeks.Conclusion Our results suggest that H9N2 （A/Guangzhou/333/99） can replicate in the murine respiratory tract without prior adaptation,and both humoral and cell‐mediated immunity play an important role in the immune response.展开更多
基于中国消费者心理特征,对1 000名中国消费者进行问卷调查,探析消费者在企业社会责任(Corporate Social Responsibility,CSR)信息刺激下,心理特征对CSR关注、CSR信任及购买意愿的影响。研究发现,心理控制源、社会自我意识、社会网络和...基于中国消费者心理特征,对1 000名中国消费者进行问卷调查,探析消费者在企业社会责任(Corporate Social Responsibility,CSR)信息刺激下,心理特征对CSR关注、CSR信任及购买意愿的影响。研究发现,心理控制源、社会自我意识、社会网络和世代观对CSR信任和购买意愿均具有显著影响,但它们对CSR关注的影响只有部分显著。根据上述四个心理特征维度对消费者进行群体细分,将消费者分为自私精明型、老实内向型、独立热情型三大类。展开更多
Fluctuations in outer space's temperature would affect the spacecraft's regular operation.This paper aims to study the temperature influences of the aluminum honeycomb buffer in the tether-net launcher.Firstly...Fluctuations in outer space's temperature would affect the spacecraft's regular operation.This paper aims to study the temperature influences of the aluminum honeycomb buffer in the tether-net launcher.Firstly,a buffer structure was designed to attenuate the pyroshock generated by the pyrotechnic device.Secondly,the mechanical properties of aluminum honeycomb at different temperatures were obtained through quasi-static compression experiments.Then,the internal ballistic responses of the launcher were gained by the closed bomb tests and the equivalent classical interior ballistic model.Finally,the recoil performance of the launcher with aluminum honeycomb buffer at different temperatures was studied.It is revealed that the aluminum honeycomb crushing force gradually decreases with the temperature increases.The peak pressure,burning rate coefficient and velocity increase while the peak time decreases with the temperature increase for the interior ballistics.For the launcher recoil responses,the average launch recoil decreases if the aluminum honeycomb doesn't enter the dense stage.The impact acceleration,projectile velocity and displacement increase as the temperature increase.The paper spotlights the temperature's influence on the recoil characteristics of the aluminum honeycomb buffer,which provides a new idea for buffering technology of pyrotechnic devices in a complex space environment.展开更多
Temporal changes in biological characteristics of small yellow croaker Larimichthys polyactis in the Yellow Sea were examined for the period of 1960–2008. The body size and age of small yellow croaker decreased subst...Temporal changes in biological characteristics of small yellow croaker Larimichthys polyactis in the Yellow Sea were examined for the period of 1960–2008. The body size and age of small yellow croaker decreased substantially, in particular, average length of fish in 2008 was reduced by ~85% than those occurring in 1985, and at that time ~93% of the total catch was dominated by one-year-old individuals. Correspondingly, growth parameters also varied significantly over the years, i.e., k（growth coefficient） and t_0（zero-length age） gradually increased from 0.26 and –0.58 year in 1960 to 0.56 and –0.25 year in 2008, respectively. Although, L∞（body length）sharply decreased from 34.21 cm in 1960 to 24.06 cm in 2008, and t_r（inflexion age） decreased from 3.78 year in1960 to 1.61 year in 2008. There was a great increase both in natural mortality coefficient and fishing mortality coefficient. However, according to the gray correlation analysis, changes in the biological characteristics of small yellow croaker were induced by different stressors ranked as： fishing vessel power〉feeding grade〉sea surface temperature. This study suggests that the active fishery management measures for biological characters of fish populations should be considered.展开更多
Based on Neumman series and epsilon-algorithm, an efficient computation for dynamic responses of systems with arbitrary time-varying characteristics is investigated. Avoiding the calculation for the inverses of the eq...Based on Neumman series and epsilon-algorithm, an efficient computation for dynamic responses of systems with arbitrary time-varying characteristics is investigated. Avoiding the calculation for the inverses of the equivalent stiffness matrices in each time step, the computation effort of the proposed method is reduced compared with the full analysis of Newmark method. The validity and applications of the proposed method are illustrated by a 4-DOF spring-mass system with periodical time-varying stiffness properties and a truss structure with arbitrary time-varying lumped mass. It shows that good approximate results can be obtained by the proposed method compared with the responses obtained by the full analysis of Newmark method.展开更多
基金the National Natural Science Foundation of China (Nos. 81622032 and 51672184)the Jiangsu Innovation and Entrepreneurship Program+1 种基金the Natural Science Research of Jiangsu Higher Education Institutions (No. 17KJA180011)the US Hermann Foundation and the National Science Foundation (award DMR-0805172) for financial supports
文摘Nanostructured diamond have potential applications in many biomedical related fields and demonstrated extraordinary capacity to influence cellular responses. Studying the surface property of nanodiamond and its influence to protein adsorption and subsequent cellular responses along with the mechanism behind such capacity becomes more important. Here the role of surface energy associated with nanostructured diamond in modulating fibronectin and osteoblast(OB, bone forming cells) responses was investigated. Nanocrystalline diamond(NCD) and submicron crystalline diamond(SMCD) films with controllable surface energy were prepared by microwave-enhanced plasma chemical vapor deposition(MPCVD) techniques. Fibronectin adsorption on the diamond films with varied surface energy values was measured via the enzyme-linked immunosorbent assay(ELISA) and the relationship between the surface energy and fibronectin adsorption was studied. The result indicated that fibronectin adsorption on nanostructured surfaces was closely related to both surface energy and material microstructures. The spreading and migration of OB aggregates(each containing 30–50 cells) on the NCD with varied surface energy values were also studied. The result indicates a correlation between the cell spreading and migration on nanodiamond and the surface energy of nanostructured surface.
基金supported by the National Basic Research Program of China (973 program: 2005CB523006)
文摘Objective To investigate the pathogenesis and immunogenicity of H9N2 influenza virus A/Guangzhou/333/99 （a reassortant of G1 and G9 viruses isolated from a female patient in 1999） in a mouse model of infection.Methods Mice were infected with increasing virus titers.Viral load in the lungs and trachea was determined by EID50 assay.Pulmonary histopathology was assessed by hematoxylin‐eosin staining.Anti‐HI antibody titers and T‐cell responses to viral HA were determined by ELISPOT and confirmed by flow cytometry.Results Mice presented a mild syndrome after intranasal infection with A/Guangzhou/333/99 （H9N2） influenza virus.Virus was detected in the trachea and lungs of mice harvested on days 3,6,and 9 post‐infection.A T‐cell response to viral HA was detected on day 6 and H9 HA‐specific CD 4＋ T‐cells predominated.Seroconversion was detected after 14 days and antibody persisted for at least 28 weeks.Conclusion Our results suggest that H9N2 （A/Guangzhou/333/99） can replicate in the murine respiratory tract without prior adaptation,and both humoral and cell‐mediated immunity play an important role in the immune response.
文摘基于中国消费者心理特征,对1 000名中国消费者进行问卷调查,探析消费者在企业社会责任(Corporate Social Responsibility,CSR)信息刺激下,心理特征对CSR关注、CSR信任及购买意愿的影响。研究发现,心理控制源、社会自我意识、社会网络和世代观对CSR信任和购买意愿均具有显著影响,但它们对CSR关注的影响只有部分显著。根据上述四个心理特征维度对消费者进行群体细分,将消费者分为自私精明型、老实内向型、独立热情型三大类。
基金supported by the National Natural Science Foundation of China(Grant No.52102436)the Fundamental Research Funds for the Central Universities(Grant No.30920021109)+3 种基金Natural Science Foundation of Jiangsu Province(BK20200496)China Postdoctoral Science Foundation(Grant No.2020M681615)the project of Key Laboratory of Impact and Safety Engineering(Ningbo University),Ministry of Education(Grant No.CJ202107)the State Key Laboratory of Mechanics and Control of Mechanical Structures(Nanjing University of Aeronautics and astronautics)(Grant No.MCMS-E-0221Y01)。
文摘Fluctuations in outer space's temperature would affect the spacecraft's regular operation.This paper aims to study the temperature influences of the aluminum honeycomb buffer in the tether-net launcher.Firstly,a buffer structure was designed to attenuate the pyroshock generated by the pyrotechnic device.Secondly,the mechanical properties of aluminum honeycomb at different temperatures were obtained through quasi-static compression experiments.Then,the internal ballistic responses of the launcher were gained by the closed bomb tests and the equivalent classical interior ballistic model.Finally,the recoil performance of the launcher with aluminum honeycomb buffer at different temperatures was studied.It is revealed that the aluminum honeycomb crushing force gradually decreases with the temperature increases.The peak pressure,burning rate coefficient and velocity increase while the peak time decreases with the temperature increase for the interior ballistics.For the launcher recoil responses,the average launch recoil decreases if the aluminum honeycomb doesn't enter the dense stage.The impact acceleration,projectile velocity and displacement increase as the temperature increase.The paper spotlights the temperature's influence on the recoil characteristics of the aluminum honeycomb buffer,which provides a new idea for buffering technology of pyrotechnic devices in a complex space environment.
基金The National Basic Research Program(973 Program)of China under contract No.2015CB453303the Aoshan Scientific and Technical Innovation Program under contract No.2015ASKJ02-05the Taishan Scholar Project Special Fund
文摘Temporal changes in biological characteristics of small yellow croaker Larimichthys polyactis in the Yellow Sea were examined for the period of 1960–2008. The body size and age of small yellow croaker decreased substantially, in particular, average length of fish in 2008 was reduced by ~85% than those occurring in 1985, and at that time ~93% of the total catch was dominated by one-year-old individuals. Correspondingly, growth parameters also varied significantly over the years, i.e., k（growth coefficient） and t_0（zero-length age） gradually increased from 0.26 and –0.58 year in 1960 to 0.56 and –0.25 year in 2008, respectively. Although, L∞（body length）sharply decreased from 34.21 cm in 1960 to 24.06 cm in 2008, and t_r（inflexion age） decreased from 3.78 year in1960 to 1.61 year in 2008. There was a great increase both in natural mortality coefficient and fishing mortality coefficient. However, according to the gray correlation analysis, changes in the biological characteristics of small yellow croaker were induced by different stressors ranked as： fishing vessel power〉feeding grade〉sea surface temperature. This study suggests that the active fishery management measures for biological characters of fish populations should be considered.
基金supported by the Foundation of the Science and Technology of Jilin Province (20070541)985-Automotive Engineering of Jilin University and Innovation Fund for 985 Engineering of Jilin University (20080104).
文摘Based on Neumman series and epsilon-algorithm, an efficient computation for dynamic responses of systems with arbitrary time-varying characteristics is investigated. Avoiding the calculation for the inverses of the equivalent stiffness matrices in each time step, the computation effort of the proposed method is reduced compared with the full analysis of Newmark method. The validity and applications of the proposed method are illustrated by a 4-DOF spring-mass system with periodical time-varying stiffness properties and a truss structure with arbitrary time-varying lumped mass. It shows that good approximate results can be obtained by the proposed method compared with the responses obtained by the full analysis of Newmark method.
