Adenosine-to-inosine(A-to-I)RNA editing,constituting nearly 90%of all RNA editing events in humans,has been reported to contribute to the tumorigenesis in diverse cancers.However,the comprehensive map for functional A...Adenosine-to-inosine(A-to-I)RNA editing,constituting nearly 90%of all RNA editing events in humans,has been reported to contribute to the tumorigenesis in diverse cancers.However,the comprehensive map for functional A-to-I RNA editing events in cancers is still insufficient.To fill this gap,we systematically and intensively analyzed multiple tumorigenic mechanisms of A-to-I RNA editing events in samples across 33 cancer types from The Cancer Genome Atlas.For individual candidate among1,500,000 quantified RNA editing events,we performed diverse types of downstream functional annotations.Finally,we identified 24,236 potentially functional A-to-I RNA editing events,including the cases in APOL1,IGFBP3,GRIA2,BLCAP,and miR-589-3p.These events might play crucial roles in the scenarios of tumorigenesis,due to their tumor-related editing frequencies or probable effects on altered expression profiles,protein functions,splicing patterns,and microRNA regulations of tumor genes.Our functional A-to-I RNA editing events(https://ccsm.uth.edu/CAeditome/)will help better understand the cancer pathology from the A-to-I RNA editing aspect.展开更多
提出一种基于(7,4)汉明码纠错机制的抗视频帧操作的帧定位算法,可通过识别含水印帧来提取受到帧操作攻击的水印信息.首先利用改进的简单线性迭代聚类(simple linear iterative clustering,SLIC)超像素分割算法提取视频帧的固有特征X,这...提出一种基于(7,4)汉明码纠错机制的抗视频帧操作的帧定位算法,可通过识别含水印帧来提取受到帧操作攻击的水印信息.首先利用改进的简单线性迭代聚类(simple linear iterative clustering,SLIC)超像素分割算法提取视频帧的固有特征X,这里X是一串7位的0-1序列,是由SLIC超像素分割算法通过预处理、量化等运算得到的聚类中心.将视频帧的固有特征X与汉明码结合形成一个特殊的编码S.以编码S为定位码,利用(7,4)汉明码的纠错机制修改1位可隐藏3位的性能来实现定位码的标识,再基于DCT-SVD(discrete cosine transformation-singular value decomposition)分解将水印信息嵌入到含定位标识的视频中.实验结果表明,该算法不仅能抵抗帧删除、帧添加和重编码等攻击,也能抵抗常见的信号处理操作.展开更多
With the development of DNA synthesis techniques,synthetic biology has enabled redesign and construction of genome on purposes(Luo et al.,2018).The whole-genome recoding of Escherichia coli could be used to enhance in...With the development of DNA synthesis techniques,synthetic biology has enabled redesign and construction of genome on purposes(Luo et al.,2018).The whole-genome recoding of Escherichia coli could be used to enhance incorporation of non-natural amino acids into proteins and to construct safer and multi-virus-resistant strains,which has always been a long-cherished wish in synthetic biology.In recent years,many research teams around the world have been engaged in genetic recoding.The Church group in Harvard University used the MAGE technique to convert 314 TAG stop codons into TAA,generating a first E.coli that is completely recoding for TAG codon(Isaacs et al.,2011).展开更多
基金supported by the National Natural Science Foundation of China(Grant No.62002270)the Fundamental Research Funds for the Central Universities,the Natural Science Foundation of Shaanxi Province of China(Grant No.2020JQ-332)+3 种基金the China Postdoctoral Science Foundation(Grant No.2018M643583)the National Natural Science Foundation of China(Grant No.82227802)the National Key R&D Program of China(Grant No.2017YFA0205202)partially funded by the National Natural Science Foundation of China(Grant No.61672422).
文摘Adenosine-to-inosine(A-to-I)RNA editing,constituting nearly 90%of all RNA editing events in humans,has been reported to contribute to the tumorigenesis in diverse cancers.However,the comprehensive map for functional A-to-I RNA editing events in cancers is still insufficient.To fill this gap,we systematically and intensively analyzed multiple tumorigenic mechanisms of A-to-I RNA editing events in samples across 33 cancer types from The Cancer Genome Atlas.For individual candidate among1,500,000 quantified RNA editing events,we performed diverse types of downstream functional annotations.Finally,we identified 24,236 potentially functional A-to-I RNA editing events,including the cases in APOL1,IGFBP3,GRIA2,BLCAP,and miR-589-3p.These events might play crucial roles in the scenarios of tumorigenesis,due to their tumor-related editing frequencies or probable effects on altered expression profiles,protein functions,splicing patterns,and microRNA regulations of tumor genes.Our functional A-to-I RNA editing events(https://ccsm.uth.edu/CAeditome/)will help better understand the cancer pathology from the A-to-I RNA editing aspect.
文摘提出一种基于(7,4)汉明码纠错机制的抗视频帧操作的帧定位算法,可通过识别含水印帧来提取受到帧操作攻击的水印信息.首先利用改进的简单线性迭代聚类(simple linear iterative clustering,SLIC)超像素分割算法提取视频帧的固有特征X,这里X是一串7位的0-1序列,是由SLIC超像素分割算法通过预处理、量化等运算得到的聚类中心.将视频帧的固有特征X与汉明码结合形成一个特殊的编码S.以编码S为定位码,利用(7,4)汉明码的纠错机制修改1位可隐藏3位的性能来实现定位码的标识,再基于DCT-SVD(discrete cosine transformation-singular value decomposition)分解将水印信息嵌入到含定位标识的视频中.实验结果表明,该算法不仅能抵抗帧删除、帧添加和重编码等攻击,也能抵抗常见的信号处理操作.
文摘With the development of DNA synthesis techniques,synthetic biology has enabled redesign and construction of genome on purposes(Luo et al.,2018).The whole-genome recoding of Escherichia coli could be used to enhance incorporation of non-natural amino acids into proteins and to construct safer and multi-virus-resistant strains,which has always been a long-cherished wish in synthetic biology.In recent years,many research teams around the world have been engaged in genetic recoding.The Church group in Harvard University used the MAGE technique to convert 314 TAG stop codons into TAA,generating a first E.coli that is completely recoding for TAG codon(Isaacs et al.,2011).
