The expression of c-met stimulated by high glucose in human renal tubular epithelial ceils and the role of HGF/c-met system in diabetic nephropathy were examined. The proximal tubular epithelial cells were cultured in...The expression of c-met stimulated by high glucose in human renal tubular epithelial ceils and the role of HGF/c-met system in diabetic nephropathy were examined. The proximal tubular epithelial cells were cultured in vitro under different conditions. MTT was used for the detection of cellular proliferation and RT-PCR was employed for measurement of c-met mRNA level. Our results showed that under different conditions, there were no significant differences in the proliferation of proximal tubular epithelial cells 12 h and 24 h after the culuture （P〉0.05）. The proliferation of proximal tubular epithelial cells showed a significant change 96 h after the culture and the cellular proliferation induced by hepatocyte growth factor （HGF） was very active （P〈0.05）. Moreover, no significant difference in the expression of c-met mRNA was found 12 h after the culture under different conditions （P〉0.05）, while 24 and 96 h after the culture, a persistent and significantly higher expression of c-met mRNA was found in HGF-induced proliferation. It is concluded that addition of exogenous HGF could inhibit the apoptosis induced by high-level glucose, promote the proliferation of proximal tubular epithelial cells, and induce the expression of c-met. Our study suggests that local up-regulation of HGF/c-met system plays an important role in the repair of renal damage in diabetic nephropathy.展开更多
文摘The expression of c-met stimulated by high glucose in human renal tubular epithelial ceils and the role of HGF/c-met system in diabetic nephropathy were examined. The proximal tubular epithelial cells were cultured in vitro under different conditions. MTT was used for the detection of cellular proliferation and RT-PCR was employed for measurement of c-met mRNA level. Our results showed that under different conditions, there were no significant differences in the proliferation of proximal tubular epithelial cells 12 h and 24 h after the culuture （P〉0.05）. The proliferation of proximal tubular epithelial cells showed a significant change 96 h after the culture and the cellular proliferation induced by hepatocyte growth factor （HGF） was very active （P〈0.05）. Moreover, no significant difference in the expression of c-met mRNA was found 12 h after the culture under different conditions （P〉0.05）, while 24 and 96 h after the culture, a persistent and significantly higher expression of c-met mRNA was found in HGF-induced proliferation. It is concluded that addition of exogenous HGF could inhibit the apoptosis induced by high-level glucose, promote the proliferation of proximal tubular epithelial cells, and induce the expression of c-met. Our study suggests that local up-regulation of HGF/c-met system plays an important role in the repair of renal damage in diabetic nephropathy.
文摘脓毒症(sepsis)在1991年初次被定义为感染所致的机体全身炎症反应综合征(SIRS)。2016年脓毒症国际共识更新至Sepsis 3.0,即由感染所致宿主免疫反应失调引起的威胁生命的器官功能障碍。在脓毒症免疫抑制阶段,程序性死亡受体1(programmed cell death protein-1,PD-1)与程序性死亡受体-配体1(programmed cell death 1 ligand 1,PD-L1)相结合能够抑制部分免疫细胞活化增殖从而达到负性调节免疫系统的作用。本综述主要围绕脓毒症中PD-1/PD-L1在T细胞、树突状细胞(DCs)、单核细胞、巨噬细胞等免疫细胞发挥免疫功能中的作用,以及对抗PD-1/PD-L1抗体疗法的应用前景进行阐述。
