AIM: To investigate the effects of Annexin A2 (ANXA2) silencing on invasion, migration, and tumorigenic potential of hepatoma cells. METHODS: Human hepatoma cell lines [HepG2, SMMC-7721, SMMC-7402, and MHCC97-H, a nov...AIM: To investigate the effects of Annexin A2 (ANXA2) silencing on invasion, migration, and tumorigenic potential of hepatoma cells. METHODS: Human hepatoma cell lines [HepG2, SMMC-7721, SMMC-7402, and MHCC97-H, a novel human hepatocellular carcinoma (HCC) cell line with high metastasis potential] and a normal hepatocyte cell line(LO2) were used in this study. The protein and mRNA expression levels of ANXA2 were analysed by western blotting and real-time polymerase chain reaction, re-spectively. The intracellular distribution profile of ANXA2 expression was determined by immunofluorescence and immunohistochemistry. Short hairpin RNA target-ing ANXA2 was designed and stably transfected into MHCC97-H cells. Cells were cultured for in vitro analy-ses or subcutaneously injected as xenografts in mice for in vivo analyses. Effects of ANXA2 silencing on cell growth were assessed by cell counting kit-8 (CCK-8) as-say (in vitro ) and tumour-growth assay (in vivo ), on cell cycling was assessed by flow cytometry and propidium iodide staining (in vitro ), and on invasion and migration potential were assessed by transwell assay and wound-healing assay, respectively (both in vitro ). RESULTS: The MHCC97-H cells, which are known to have high metastasis potential, showed the highest lev-el of ANXA2 expression among the four HCC cell types examined; compared to the LO2 cells, the MHCC97-H expression level was 8-times higher. The ANXA2 expres-sion was effectively inhibited (about 80%) by ANXA2-specific small hairpin RNA (shRNA). ANXA2 expression in the MHCC97-H cells was mainly localized to the cel-lular membrane and cytoplasm, and some localization was detected in the nucleus. Moreover, the proliferation of MHCC97-H cells was obviously suppressed by shR-NA-mediated ANXA2 silencing in vitro , and the tumour growth inhibition rate was 38.24% in vivo . The per-centage of MHCC97-H cells in S phase dramatically de-creased (to 27.76%) under ANXA2-silenced conditions. Furthermore, ANXA2-silenced MHCC97-H cells showed lower invasiveness展开更多
The fall armyworm Spodoptera frugiperda,an invasive insect pest native to the Americas,has established populations throughout eastern China.The North China Plain一a key corn-producing area in East China with a unique ...The fall armyworm Spodoptera frugiperda,an invasive insect pest native to the Americas,has established populations throughout eastern China.The North China Plain一a key corn-producing area in East China with a unique topography一was invaded by fall armyworm in 2019 and is seriously threatened by this migratory pest.However,the spatiotemporal extent of the migratory movements of fall armyworm from the North China Plain remains poorly understood.Using an air transport-based trajectory modeling approach that incorporates flight behavior,we simulated the potential nocturnal migration trajectories of fall armyworm from the North China Plain based on historical meteorological data from June to October of 2015-2019,and examined the night-time atmospheric conditions associated with their possible flights.The emigration patterns showed monthly variation in the main landing area and common migration direction.The displacement of newly emerged moths from the North China Plain was concentrated in the Northeast China Plain(including Liaoning,Jilin and Heilongjiang provinces)before late summer,after which they were most likely to undertake return flights to the south(especially into Hubei,Anhui and Hunan provinces).This southwest-northeast aerial migration corridor follows the topography of East China and is affected by the East Asian monsoon.These topographic-atmospheric conditions have resulted in the North China Plain becoming a key stopover for fall armyworm populations engaging in multigenerational long-distance migration across East China.These findings contribute to our knowledge of fall army-worm migration and will aid in the implementation of management and control strategies against this highly migratory agricultural pest.展开更多
基金Supported by The Society Development of Nantong,No.HS2012034the Jiangsu Health Projects,No.BL2012053 and No.K201102+1 种基金the Priority Academic Program Development of Jiangsuthe International S and T Cooperation Program of China,No.2013DFA32150
文摘AIM: To investigate the effects of Annexin A2 (ANXA2) silencing on invasion, migration, and tumorigenic potential of hepatoma cells. METHODS: Human hepatoma cell lines [HepG2, SMMC-7721, SMMC-7402, and MHCC97-H, a novel human hepatocellular carcinoma (HCC) cell line with high metastasis potential] and a normal hepatocyte cell line(LO2) were used in this study. The protein and mRNA expression levels of ANXA2 were analysed by western blotting and real-time polymerase chain reaction, re-spectively. The intracellular distribution profile of ANXA2 expression was determined by immunofluorescence and immunohistochemistry. Short hairpin RNA target-ing ANXA2 was designed and stably transfected into MHCC97-H cells. Cells were cultured for in vitro analy-ses or subcutaneously injected as xenografts in mice for in vivo analyses. Effects of ANXA2 silencing on cell growth were assessed by cell counting kit-8 (CCK-8) as-say (in vitro ) and tumour-growth assay (in vivo ), on cell cycling was assessed by flow cytometry and propidium iodide staining (in vitro ), and on invasion and migration potential were assessed by transwell assay and wound-healing assay, respectively (both in vitro ). RESULTS: The MHCC97-H cells, which are known to have high metastasis potential, showed the highest lev-el of ANXA2 expression among the four HCC cell types examined; compared to the LO2 cells, the MHCC97-H expression level was 8-times higher. The ANXA2 expres-sion was effectively inhibited (about 80%) by ANXA2-specific small hairpin RNA (shRNA). ANXA2 expression in the MHCC97-H cells was mainly localized to the cel-lular membrane and cytoplasm, and some localization was detected in the nucleus. Moreover, the proliferation of MHCC97-H cells was obviously suppressed by shR-NA-mediated ANXA2 silencing in vitro , and the tumour growth inhibition rate was 38.24% in vivo . The per-centage of MHCC97-H cells in S phase dramatically de-creased (to 27.76%) under ANXA2-silenced conditions. Furthermore, ANXA2-silenced MHCC97-H cells showed lower invasiveness
基金the National Natural Science Foundation of China(31901873 and 31727901)the China Postdoctoral Science Foundation(2019M660896)+1 种基金the Central Public-interest Scientific Institution Basal Research Fund,China(CAAS-ZDRW202007,Y2019YJ06)the National Key R&D Program of China(2019YFD0300105).
文摘The fall armyworm Spodoptera frugiperda,an invasive insect pest native to the Americas,has established populations throughout eastern China.The North China Plain一a key corn-producing area in East China with a unique topography一was invaded by fall armyworm in 2019 and is seriously threatened by this migratory pest.However,the spatiotemporal extent of the migratory movements of fall armyworm from the North China Plain remains poorly understood.Using an air transport-based trajectory modeling approach that incorporates flight behavior,we simulated the potential nocturnal migration trajectories of fall armyworm from the North China Plain based on historical meteorological data from June to October of 2015-2019,and examined the night-time atmospheric conditions associated with their possible flights.The emigration patterns showed monthly variation in the main landing area and common migration direction.The displacement of newly emerged moths from the North China Plain was concentrated in the Northeast China Plain(including Liaoning,Jilin and Heilongjiang provinces)before late summer,after which they were most likely to undertake return flights to the south(especially into Hubei,Anhui and Hunan provinces).This southwest-northeast aerial migration corridor follows the topography of East China and is affected by the East Asian monsoon.These topographic-atmospheric conditions have resulted in the North China Plain becoming a key stopover for fall armyworm populations engaging in multigenerational long-distance migration across East China.These findings contribute to our knowledge of fall army-worm migration and will aid in the implementation of management and control strategies against this highly migratory agricultural pest.
