Mature eggs of Oreochromis aurea were obtained with artificial reproduction technology, and the hybrids were acquired with Siniperca chuatsi (♂) using artificial insemination . Genomic DNA of O. aurea (♀), S. chuats...Mature eggs of Oreochromis aurea were obtained with artificial reproduction technology, and the hybrids were acquired with Siniperca chuatsi (♂) using artificial insemination . Genomic DNA of O. aurea (♀), S. chuatsi (♂) and their progeny were examined using RAPD method. Only 2 primers (S336, OPZ06) of screened 11 random primers could be amplified into two specifically RAPD bands on progeny which were the same as those of S. chuatsi (♂) and did not exist in O. aurea (♀), and other amplified bands on progeny were all from O. aurea (♀). Genetic similarity index was 0.858 between the progeny and O. aurea (♀), while that was (0.049) between the progeny and S. chuatsi (♂). This indicated that the genetic substance of progeny was mostly from O. aurea (♀). The primary function of sperm from S. chuatsi (♂) was stimulating development of eggs from O. aurea (♀), at the same time little genetic substance of S.chuatsi(♂) appeared in the progeny, which laid the foundation for breeding of fine varieties having characters of O. aurea (♀) and S. chuatsi (♂).展开更多
Outcrossing rate in a natural population of Caldesia grandis was estimated by the dominant random amplified polymorphism DNA （RAPD） marker using 10 open-pollinated progeny arrays of 24 individuals. The multilocus ou...Outcrossing rate in a natural population of Caldesia grandis was estimated by the dominant random amplified polymorphism DNA （RAPD） marker using 10 open-pollinated progeny arrays of 24 individuals. The multilocus outcrossing rate estimated based on all 25 RAPD loci was 0.872 ±0.033 and the single-locus outcrossing rate was 0.795 ±0.032. Multilocus esti- mates did not differ significantly from the single-locus estimates. The fixation index, F, in the progeny estimated from RAPD data was -0.142 ±0.000. The estimates of multilocus outcrossing rates （tm） and single-locus outcrossing rates （ts） obtained from MLDT clearly indicate that outcrossing is predominant in the open-pollinated C. grandis population. An empirical analysis suggests that 15 should be the minimum number of dominant marker loci necessary to achieve robust estimates of tm.展开更多
文摘Mature eggs of Oreochromis aurea were obtained with artificial reproduction technology, and the hybrids were acquired with Siniperca chuatsi (♂) using artificial insemination . Genomic DNA of O. aurea (♀), S. chuatsi (♂) and their progeny were examined using RAPD method. Only 2 primers (S336, OPZ06) of screened 11 random primers could be amplified into two specifically RAPD bands on progeny which were the same as those of S. chuatsi (♂) and did not exist in O. aurea (♀), and other amplified bands on progeny were all from O. aurea (♀). Genetic similarity index was 0.858 between the progeny and O. aurea (♀), while that was (0.049) between the progeny and S. chuatsi (♂). This indicated that the genetic substance of progeny was mostly from O. aurea (♀). The primary function of sperm from S. chuatsi (♂) was stimulating development of eggs from O. aurea (♀), at the same time little genetic substance of S.chuatsi(♂) appeared in the progeny, which laid the foundation for breeding of fine varieties having characters of O. aurea (♀) and S. chuatsi (♂).
基金Supported by the State Key Basic Research and Develop-ment Plan (G2000046805)the National Natural Science Foundation of China (30370098, 30570111)
文摘Outcrossing rate in a natural population of Caldesia grandis was estimated by the dominant random amplified polymorphism DNA （RAPD） marker using 10 open-pollinated progeny arrays of 24 individuals. The multilocus outcrossing rate estimated based on all 25 RAPD loci was 0.872 ±0.033 and the single-locus outcrossing rate was 0.795 ±0.032. Multilocus esti- mates did not differ significantly from the single-locus estimates. The fixation index, F, in the progeny estimated from RAPD data was -0.142 ±0.000. The estimates of multilocus outcrossing rates （tm） and single-locus outcrossing rates （ts） obtained from MLDT clearly indicate that outcrossing is predominant in the open-pollinated C. grandis population. An empirical analysis suggests that 15 should be the minimum number of dominant marker loci necessary to achieve robust estimates of tm.
