目的建立桂枝茯苓胶囊（GFC）三萜类成分UPLC指纹图谱方法,为评价GFC的质量提供新方法。方法采用Agilent Zorbox Eclipse Plus HD C18（100 mm×2.1 mm,1.8μm）色谱柱,以乙腈-0.1%磷酸水溶液为流动相,梯度洗脱,体积流量0.2 m L/min...目的建立桂枝茯苓胶囊（GFC）三萜类成分UPLC指纹图谱方法,为评价GFC的质量提供新方法。方法采用Agilent Zorbox Eclipse Plus HD C18（100 mm×2.1 mm,1.8μm）色谱柱,以乙腈-0.1%磷酸水溶液为流动相,梯度洗脱,体积流量0.2 m L/min,柱温30℃,检测波长为210 nm。采用Q-TOF/MS对指纹图谱中共有峰进行指认。结果得到分离度和重复性良好的GFC三萜类成分指纹图谱,确定了20个共有峰,其中1-7、10、12、14-17号峰共13个共有峰来自于茯苓,8、11号峰来自于桂枝,9号峰来自于白芍和牡丹皮,13号峰来自于桃仁、白芍、牡丹皮和桂枝。10批成品指纹图谱相似度在0.90以上。UPLC-Q-TOF/MS共鉴定出15个成分,分别为常春藤皂苷（1）、去氢土莫酸（2）、土莫酸（3）、猪苓酸C（4）、3-表去氢土莫酸（6）、茯苓酸D（7）、α-亚麻酸（9）、去氢茯苓酸（10）、齐墩果酸（11）、茯苓酸（12）、亚油酸（13）、棕榈油酸甲酯（15）、棕榈酸（16）、棕榈酸乙酯（17）、胡萝卜苷（18）。结论建立的UPLC指纹图谱有较好的精密度、重复性和稳定性,适用于GFC的质量控制。展开更多
Objective With the improvement of people＇s living standards, people＇s requests for beauty are increasing. Skin whitening and lightening have become the pursuit of many women, and whitening and removing freckles have...Objective With the improvement of people＇s living standards, people＇s requests for beauty are increasing. Skin whitening and lightening have become the pursuit of many women, and whitening and removing freckles have become the focus of scientific research. At present, widely used whitening agents, such as kojic acid, vitamin C, and its derivatives, have shortcomings such as poor stability and retarded effect. Therefore, safer and more effective whitening products from herbs are urgently needed. To explore the possibility of triterpenes as whitening active substance, the effects of total triterpenes of Poria（TTP） and poricoic acid A（PAA） on mushroom tyrosinase activities and B16 cells were investigated, and their mechanisms on mushroom tyrosinase were also studied. Methods Using arbutin and nicotinamide as reference substances, we determinated the inhibitory effects of TTP and PAA on mushroom tyrosinase and tyrosinase in B16 cells and then studied the inhibitory mechanism on mushroom tyrosinase. Results TTP and PAA exhibited good inhibitory effects on the activities of monophenolase and diphenolase in mushroom tyrosinase, as well as a certain inhibitory effect on tyrosinase in B16 cells. Conclusion TTP and PAA are potential whitening active ingredients.展开更多
文摘目的建立桂枝茯苓胶囊（GFC）三萜类成分UPLC指纹图谱方法,为评价GFC的质量提供新方法。方法采用Agilent Zorbox Eclipse Plus HD C18（100 mm×2.1 mm,1.8μm）色谱柱,以乙腈-0.1%磷酸水溶液为流动相,梯度洗脱,体积流量0.2 m L/min,柱温30℃,检测波长为210 nm。采用Q-TOF/MS对指纹图谱中共有峰进行指认。结果得到分离度和重复性良好的GFC三萜类成分指纹图谱,确定了20个共有峰,其中1-7、10、12、14-17号峰共13个共有峰来自于茯苓,8、11号峰来自于桂枝,9号峰来自于白芍和牡丹皮,13号峰来自于桃仁、白芍、牡丹皮和桂枝。10批成品指纹图谱相似度在0.90以上。UPLC-Q-TOF/MS共鉴定出15个成分,分别为常春藤皂苷（1）、去氢土莫酸（2）、土莫酸（3）、猪苓酸C（4）、3-表去氢土莫酸（6）、茯苓酸D（7）、α-亚麻酸（9）、去氢茯苓酸（10）、齐墩果酸（11）、茯苓酸（12）、亚油酸（13）、棕榈油酸甲酯（15）、棕榈酸（16）、棕榈酸乙酯（17）、胡萝卜苷（18）。结论建立的UPLC指纹图谱有较好的精密度、重复性和稳定性,适用于GFC的质量控制。
基金Project of National Twelve-Five Year Research Program of China(2011BAI06B03-2 and 2012BAI29B03)
文摘Objective With the improvement of people＇s living standards, people＇s requests for beauty are increasing. Skin whitening and lightening have become the pursuit of many women, and whitening and removing freckles have become the focus of scientific research. At present, widely used whitening agents, such as kojic acid, vitamin C, and its derivatives, have shortcomings such as poor stability and retarded effect. Therefore, safer and more effective whitening products from herbs are urgently needed. To explore the possibility of triterpenes as whitening active substance, the effects of total triterpenes of Poria（TTP） and poricoic acid A（PAA） on mushroom tyrosinase activities and B16 cells were investigated, and their mechanisms on mushroom tyrosinase were also studied. Methods Using arbutin and nicotinamide as reference substances, we determinated the inhibitory effects of TTP and PAA on mushroom tyrosinase and tyrosinase in B16 cells and then studied the inhibitory mechanism on mushroom tyrosinase. Results TTP and PAA exhibited good inhibitory effects on the activities of monophenolase and diphenolase in mushroom tyrosinase, as well as a certain inhibitory effect on tyrosinase in B16 cells. Conclusion TTP and PAA are potential whitening active ingredients.
