采用基于Illumina Hi Seq测序平台的高通量测序技术,对拟穴青蟹(Scylla paramamosain)肠道及其养殖池塘水体、底泥中细菌种类及丰度进行了研究。测序结果显示,3个样品共获得有效序列234575条,可聚类于2812个分类操作单元(OTUs),归属于...采用基于Illumina Hi Seq测序平台的高通量测序技术,对拟穴青蟹(Scylla paramamosain)肠道及其养殖池塘水体、底泥中细菌种类及丰度进行了研究。测序结果显示,3个样品共获得有效序列234575条,可聚类于2812个分类操作单元(OTUs),归属于拟穴青蟹肠道、养殖水体、池塘底泥样品的操作分类单元(OTU)个数分别为453、706和2547,其中有184个OTU均能在3个样品中检测到,在青蟹肠道和养殖水体、青蟹肠道和池塘底泥中分别检测到197和309个共有OTU。物种注释结果显示,拟穴青蟹肠道中优势细菌种类为变形菌门(Proteobacteria)(39.96%)、柔膜菌门(Tenericutes)(23.09%)和厚壁菌门(Firmicutes)(16.58%);养殖水体中优势细菌种类为变形菌门(63.02%)、放线菌门(Actinobacteria)(24.96%)和拟杆菌门(Bacteroidetes)(8.41%);池塘底泥中优势细菌种类为变形菌门(75.23%)、拟杆菌门(5.72%)和放线菌门(3.83%)。此外,对各样品中丰度最高的前10位OTU分析显示,不同样品中占优势地位的10种细菌在数据库(SILVA)缺乏相关已知序列,并且各样品中的优势细菌种类完全不同。实验结果表明拟穴青蟹肠道与其池塘养殖环境中菌群结构存在着密切的相关性,但肠道菌群同时具有一定的独立性,其优势细菌种类与养殖环境中优势细菌种类无关。本研究旨在为拟穴青蟹健康养殖和微生态调控提供实验依据。展开更多
Forest ecosystems help conserve the quality of water resources in aquatic habitats. The conservation of biological diversity in aquatic and terrestrial ecosystems remains a communal concern. Aquatic ecosystems and res...Forest ecosystems help conserve the quality of water resources in aquatic habitats. The conservation of biological diversity in aquatic and terrestrial ecosystems remains a communal concern. Aquatic ecosystems and resources are vulnerable but can be preserved and protected by forests. In sub-Saharan regions of Africa, water from ecosystems such as ponds still play important role in the livelihood of local populations. Water from temporary ponds is used by local populations for multiple needs; however, in this part of the world, the population is increasing, thus increasing human needs and activities and land use in the region. Land-use changes lead to deforestation, land degradation and the decline in freshwater, affecting human health and well-being. Forest degradation leads to the decline in ecosystem goods and services, particularly those related to watersheds. This study conducted in eastern Burkina Faso aimed to assess water quality of temporary ponds in protected forest areas (reserves) and surrounding villages. It was conducted in 61 temporary ponds where physical, chemical and biological variables were measured, such as water surface area, depth, transparency, macrophyte cover, pH, dissolved O<sub>2</sub>, conductivity, nutrient concentrations and algae biomass. The results showed that at p < 5%, water surface area (p = 0.02), depth (p = 0.00), nutrient content (p = 0.00), and algae biomass (p = 0.04) were significantly higher outside reserves than inside reserves. In contrast, macrophyte cover (mean cover percentages 53 vs. 44.5%) and water transparency (p = 0.02) were higher inside reserves. The variations in conductivity and pH were not significant. All trends showed the influences of human activities on water characteristics and the role forests and land cover had in preventing negative human impacts and disturbance of temporary ponds. Forests and land cover are important to water quality conservation and algae biomass regulation in temporary ponds. Protecting and managing forests is there展开更多
The pond water is used by a variety of animals i.e. mammals, birds, duck, and fish. Sediments play a significant role in determining the overall environmental quality for the living organism. Therefore, in this work, ...The pond water is used by a variety of animals i.e. mammals, birds, duck, and fish. Sediments play a significant role in determining the overall environmental quality for the living organism. Therefore, in this work, chemical characterization of pond water and sediment in the largest coal burning basin of India i.e. Korba basin is described. Elements i.e. C, O, S, F, Cl, Na, Mg, Al, Si, P, K, Ca, Ti, Fe, As, Cr, Cu, Zn, Cd, Pb, and Hg were quantified. Toxic metals i.e. As, Cd, Hg, and Pb were highly enriched in the sediments, ranging from 36 - 154, 0.14 - 1.19, 0.12 - 0.82 and 26 - 127 mg/kg with mean value (p = 0.05) of 95 ± 12, 0.62 ± 0.11, 0.35 ± 0.08 and 75 ± 13 mg/kg, respectively. The concentration variations, pollution indices and sources of elements in water and sediment are discussed.展开更多
文摘采用基于Illumina Hi Seq测序平台的高通量测序技术,对拟穴青蟹(Scylla paramamosain)肠道及其养殖池塘水体、底泥中细菌种类及丰度进行了研究。测序结果显示,3个样品共获得有效序列234575条,可聚类于2812个分类操作单元(OTUs),归属于拟穴青蟹肠道、养殖水体、池塘底泥样品的操作分类单元(OTU)个数分别为453、706和2547,其中有184个OTU均能在3个样品中检测到,在青蟹肠道和养殖水体、青蟹肠道和池塘底泥中分别检测到197和309个共有OTU。物种注释结果显示,拟穴青蟹肠道中优势细菌种类为变形菌门(Proteobacteria)(39.96%)、柔膜菌门(Tenericutes)(23.09%)和厚壁菌门(Firmicutes)(16.58%);养殖水体中优势细菌种类为变形菌门(63.02%)、放线菌门(Actinobacteria)(24.96%)和拟杆菌门(Bacteroidetes)(8.41%);池塘底泥中优势细菌种类为变形菌门(75.23%)、拟杆菌门(5.72%)和放线菌门(3.83%)。此外,对各样品中丰度最高的前10位OTU分析显示,不同样品中占优势地位的10种细菌在数据库(SILVA)缺乏相关已知序列,并且各样品中的优势细菌种类完全不同。实验结果表明拟穴青蟹肠道与其池塘养殖环境中菌群结构存在着密切的相关性,但肠道菌群同时具有一定的独立性,其优势细菌种类与养殖环境中优势细菌种类无关。本研究旨在为拟穴青蟹健康养殖和微生态调控提供实验依据。
基金BIOLOG Program of the German Ministry of Education and Science(BMB+F BIOTA Ⅲ)
文摘Forest ecosystems help conserve the quality of water resources in aquatic habitats. The conservation of biological diversity in aquatic and terrestrial ecosystems remains a communal concern. Aquatic ecosystems and resources are vulnerable but can be preserved and protected by forests. In sub-Saharan regions of Africa, water from ecosystems such as ponds still play important role in the livelihood of local populations. Water from temporary ponds is used by local populations for multiple needs; however, in this part of the world, the population is increasing, thus increasing human needs and activities and land use in the region. Land-use changes lead to deforestation, land degradation and the decline in freshwater, affecting human health and well-being. Forest degradation leads to the decline in ecosystem goods and services, particularly those related to watersheds. This study conducted in eastern Burkina Faso aimed to assess water quality of temporary ponds in protected forest areas (reserves) and surrounding villages. It was conducted in 61 temporary ponds where physical, chemical and biological variables were measured, such as water surface area, depth, transparency, macrophyte cover, pH, dissolved O<sub>2</sub>, conductivity, nutrient concentrations and algae biomass. The results showed that at p < 5%, water surface area (p = 0.02), depth (p = 0.00), nutrient content (p = 0.00), and algae biomass (p = 0.04) were significantly higher outside reserves than inside reserves. In contrast, macrophyte cover (mean cover percentages 53 vs. 44.5%) and water transparency (p = 0.02) were higher inside reserves. The variations in conductivity and pH were not significant. All trends showed the influences of human activities on water characteristics and the role forests and land cover had in preventing negative human impacts and disturbance of temporary ponds. Forests and land cover are important to water quality conservation and algae biomass regulation in temporary ponds. Protecting and managing forests is there
文摘The pond water is used by a variety of animals i.e. mammals, birds, duck, and fish. Sediments play a significant role in determining the overall environmental quality for the living organism. Therefore, in this work, chemical characterization of pond water and sediment in the largest coal burning basin of India i.e. Korba basin is described. Elements i.e. C, O, S, F, Cl, Na, Mg, Al, Si, P, K, Ca, Ti, Fe, As, Cr, Cu, Zn, Cd, Pb, and Hg were quantified. Toxic metals i.e. As, Cd, Hg, and Pb were highly enriched in the sediments, ranging from 36 - 154, 0.14 - 1.19, 0.12 - 0.82 and 26 - 127 mg/kg with mean value (p = 0.05) of 95 ± 12, 0.62 ± 0.11, 0.35 ± 0.08 and 75 ± 13 mg/kg, respectively. The concentration variations, pollution indices and sources of elements in water and sediment are discussed.
