BACKGROUND： Along with aging, antioxidase activity decreases and oxygen-derived free radicals greatly accumulate, resulting in cellular senescence, or even cell death. This is manifested by hypomnesia and disordered ...BACKGROUND： Along with aging, antioxidase activity decreases and oxygen-derived free radicals greatly accumulate, resulting in cellular senescence, or even cell death. This is manifested by hypomnesia and disordered metabolism of free radicals. Studies have reported that Longyanshen polysaccharides have the function of antioxidation and improved brain memory. OBJECTIVE： To observe the effects of Longyanshen polysaccharides on free radical metabolism in brain tissue to verify the anti-aging mechanisms in senescence accelerated-prone （SAMP8） mice. DESIGN, TIME AND SETTING： The randomized, controlled, biochemical experiment was performed in the Department of Pharmacology and Scientific Experimental Center of Guangxi Medical University （China） from September 2005 to January 2008. MATERIALS： Forty SAMP8 mice were randomized into four groups： SAMP8 control group, as well as low-, mid-, and high-dose polysaccharide, with 10 mice in each group. Ten senescence accelerated-resistantprone （SAMR 1） mice served as the normal control group. Longyanshen polysaccharides, extracted from the medical plant Longyanshen, were supplied by the Department of Pharmacology, Guangxi Medical University Superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px）, malonaldehyde （MDA）, nitric oxide （NO）, and total protein test kitwere purchased from Nanjing Jiancheng Bioengineering Institute （China）. METHODS： SAMP8 mice were used to establish a dementia animal model. SAMP8 and SAMR1 control mice were administered 30 mL/kg saline. The low-, middle-, and high-dose polysaccharide groups were administered 45, 90, and 180 mg/kg Longyanshen polysaccharides, respectively. Each group was treated by intragastric administration, once daily, for 50 continuous days. MAIN OUTCOME MEASURES： One hour after the last administration, mouse brain tissues were collected, and retro orbital blood sampling was performed. Spectrophotometry was used to measure SOD and GSH-Px activity, as well as MDA and NO concentration in展开更多
通过超声水提醇沉的方法,以得率为指标,优化党参多糖的提取工艺,并使用党参多糖水溶液与荞麦醋、蜂蜜配制成醋饮料。以单因素和响应面法确定最佳提取工艺,并以正交实验对党参荞麦保健醋饮料的口感配方进行优化。结果表明,党参多糖最佳...通过超声水提醇沉的方法,以得率为指标,优化党参多糖的提取工艺,并使用党参多糖水溶液与荞麦醋、蜂蜜配制成醋饮料。以单因素和响应面法确定最佳提取工艺,并以正交实验对党参荞麦保健醋饮料的口感配方进行优化。结果表明,党参多糖最佳提取工艺为料液比1∶30(g/m L),超声时间60 min,超声温度60℃,醇沉浓度60%,得率为18.03%±0.45%。保健饮料最佳配比为党参多糖水溶液100 m L,荞麦醋为1 m L,蜂蜜12 g,感官得分为84.2分。应用此配方加工的饮料口感酸甜柔和,风味独特,且各项指标均符合国家醋饮料的相关规定(GB/T 30884-2014),可在实际生产中加以应用。展开更多
基金Supported by: Guangxi Natural Science Foundation, No. 0447030Guangxi Scientific Research and Technological Development Projects, No. 0630002-2A
文摘BACKGROUND： Along with aging, antioxidase activity decreases and oxygen-derived free radicals greatly accumulate, resulting in cellular senescence, or even cell death. This is manifested by hypomnesia and disordered metabolism of free radicals. Studies have reported that Longyanshen polysaccharides have the function of antioxidation and improved brain memory. OBJECTIVE： To observe the effects of Longyanshen polysaccharides on free radical metabolism in brain tissue to verify the anti-aging mechanisms in senescence accelerated-prone （SAMP8） mice. DESIGN, TIME AND SETTING： The randomized, controlled, biochemical experiment was performed in the Department of Pharmacology and Scientific Experimental Center of Guangxi Medical University （China） from September 2005 to January 2008. MATERIALS： Forty SAMP8 mice were randomized into four groups： SAMP8 control group, as well as low-, mid-, and high-dose polysaccharide, with 10 mice in each group. Ten senescence accelerated-resistantprone （SAMR 1） mice served as the normal control group. Longyanshen polysaccharides, extracted from the medical plant Longyanshen, were supplied by the Department of Pharmacology, Guangxi Medical University Superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px）, malonaldehyde （MDA）, nitric oxide （NO）, and total protein test kitwere purchased from Nanjing Jiancheng Bioengineering Institute （China）. METHODS： SAMP8 mice were used to establish a dementia animal model. SAMP8 and SAMR1 control mice were administered 30 mL/kg saline. The low-, middle-, and high-dose polysaccharide groups were administered 45, 90, and 180 mg/kg Longyanshen polysaccharides, respectively. Each group was treated by intragastric administration, once daily, for 50 continuous days. MAIN OUTCOME MEASURES： One hour after the last administration, mouse brain tissues were collected, and retro orbital blood sampling was performed. Spectrophotometry was used to measure SOD and GSH-Px activity, as well as MDA and NO concentration in
文摘通过超声水提醇沉的方法,以得率为指标,优化党参多糖的提取工艺,并使用党参多糖水溶液与荞麦醋、蜂蜜配制成醋饮料。以单因素和响应面法确定最佳提取工艺,并以正交实验对党参荞麦保健醋饮料的口感配方进行优化。结果表明,党参多糖最佳提取工艺为料液比1∶30(g/m L),超声时间60 min,超声温度60℃,醇沉浓度60%,得率为18.03%±0.45%。保健饮料最佳配比为党参多糖水溶液100 m L,荞麦醋为1 m L,蜂蜜12 g,感官得分为84.2分。应用此配方加工的饮料口感酸甜柔和,风味独特,且各项指标均符合国家醋饮料的相关规定(GB/T 30884-2014),可在实际生产中加以应用。
