Desert ephemeral plants play an important role in desert ecosystem.Soil water availability is considered as the major restrictive factor limiting the growth of ephemeral plants.Moreover,arbuscular mycorrhizal fungi（A...Desert ephemeral plants play an important role in desert ecosystem.Soil water availability is considered as the major restrictive factor limiting the growth of ephemeral plants.Moreover,arbuscular mycorrhizal fungi（AM fungi） are widely reported to improve the growth of desert ephemerals.The present study aimed to test the hypothesis of that AM fungi could alleviate drought stress of desert ephemeral Plantago minuta,and AM fungal functions reduced with the improvement of soil water content.A pot experiment was carried out with three levels of soil water contents（4.5%,9.0%,and 15.8%（w/w））,and three AM inoculation treatments（Glomus mosseae,Glomus etunicatum and non-inoculation）.The results indicate that mycorrhizal colonization rate decreased with the increase of soil water availability.Inoculation improved plant growth and N,P and K acquisition in both shoots and roots regardless water treatments.When comparing the two fungi,plants inoculated with G.mosseae performed better than those inoculated with G.etunicatum in terms of plant growth and nutrient acquisition.These results showed that ameliorative soil water did not suppress arbuscular mycorrhizal fungal functions in improving growth and nutrient acquisition of desert ephemeral Plantago minuta.展开更多
Two phenylethanoid glycosides （PhGs） were isolated and purified from the aerial parts of Plantago asiatica for the first time by high performance centrifugal partition chromatography （HPCPC） using ethyl acetate-n-...Two phenylethanoid glycosides （PhGs） were isolated and purified from the aerial parts of Plantago asiatica for the first time by high performance centrifugal partition chromatography （HPCPC） using ethyl acetate-n-butanol-ethanol-water （0.5：0.5：0.1：1, v/v/v/v）. A total of 45.6 mg of compound 1 and 293.8 mg of compound 2 were purified from 1341 mg of the n-butanol extract of P. asiatica. The structures of the two PhGs were tentatively identified as plantamajoside and aeteoside or isoacteoside by eleetrospray ionization multi stage tandem mass spectrometry （ESI-MS^n） in the negative ion mode.展开更多
Two phenylethanoid glycosides（PhGs）, plantamajoside and acteoside, were isolated and purified from the aerial parts ofPlantago asiatica for the first time by high performance centrifugal partition chromatography（HP...Two phenylethanoid glycosides（PhGs）, plantamajoside and acteoside, were isolated and purified from the aerial parts ofPlantago asiatica for the first time by high performance centrifugal partition chromatography（HPCPC） with ethyl acetate, n-butanol, ethanol and water（0.5：0.5：0.1：1, volume ratio） as solvent system. A total of 45.6 mg of plantamajoside and 293.8 mg of acteoside were purified from 1341 mg of the n-butanol extract of P. asiatica, with a purity of 〉93.3% as determined by HPLC. The HPCPC fractions were analyzed by HPLC-DAD and the structures were identified by their retention time, UV, electrospray ionization multi stage tandem mass spectrometry（ESI-MSn） in the negative ion mode, and confirmed by NMR experiments. The characteristic fragment ions of ESI-MS of the two PhGs isolated from Plantago asiatica were discussed, which are specific and useful for the identification of the structures of PhGs.展开更多
本研究采用响应面设计优化超声辅助提取车前总黄酮的最佳条件,然后用此条件提取大车前和平车前总黄酮,并探究大孔树脂纯化对三种车前草总黄酮抗氧化活性的影响。结果表明,在超声温度60℃、乙醇浓度70%的条件下,车前总黄酮最佳提取工艺...本研究采用响应面设计优化超声辅助提取车前总黄酮的最佳条件,然后用此条件提取大车前和平车前总黄酮,并探究大孔树脂纯化对三种车前草总黄酮抗氧化活性的影响。结果表明,在超声温度60℃、乙醇浓度70%的条件下,车前总黄酮最佳提取工艺参数为液料比20:1 m L/g、超声时间80 min、超声功率210 W,车前、大车前和平车前的总黄酮得率分别为5.04%、2.86%和1.22%。无论是纯化前还是纯化后,大车前总黄酮的还原力和对羟基自由基的清除作用最强,平车前最弱;车前总黄酮对DPPH自由基的清除作用最大,平车前最弱。纯化前后的还原力和对DPPH自由基、羟基自由基的清除作用都接近Vc的水平。展开更多
Four new indole alkaloids,plasiaticines A-D(1-4),together with two known ones,were isolated from the seeds of Plantago asiatica.The structures of the new compounds were elucidated on the basis of comprehensive analysi...Four new indole alkaloids,plasiaticines A-D(1-4),together with two known ones,were isolated from the seeds of Plantago asiatica.The structures of the new compounds were elucidated on the basis of comprehensive analysis of spectroscopic data.All compounds were tested for their cytotoxic activity,and all compounds except 4 were tested for their acetylcholinesterase(AChE)inhibitory activities.展开更多
[Objectives]To explore the pharmacodynamic material basis of Sijicao granules for the treatment of eczema through chemical composition-network pharmacology.[Methods]First of all,the chemical constituents of Polygonum ...[Objectives]To explore the pharmacodynamic material basis of Sijicao granules for the treatment of eczema through chemical composition-network pharmacology.[Methods]First of all,the chemical constituents of Polygonum capitatum and Plantago asiatica from Sijicao granules were collected,and the relevant target information of the constituents was collected by TCMSP,PubChem,DisGeNET,GeneCards and STRING databases.Furthermore,Cytoscape 3.8.2 software was used to construct the chemical compounds-target network map of Sijicao granules.Finally,STRING database was used for PPI protein network analysis,GO functional enrichment analysis and KEGG pathway enrichment analysis of core targets,and molecular docking between core constituents and protein targets was also performed.[Results]30 constituents,including quercetin,kaempferol,luteolin,ellagic acid and gallic acid,were discovered to be the key effective compounds of Sijicao granules in the treatment of eczema.And its core action protein targets were PTGS2,NOS2,AKT1,TP53,IL6,HMOX1.What s more,through GO functional enrichment analysis of biological process(BP),cell component(CC),molecular function(MF)analysis and KEGG pathway enrichment analysis,the main pathways of action of Sijicao granules for the treatment of eczema including IL-17 signaling pathway,T cell receptor signaling pathway,cancer signaling pathway,TNF signaling pathway and Relaxin signaling pathway.In addition,molecular docking results displayed that the primary active constituents quercetin,kaempferol and luteolin were well combined with the core protein targets AKT1 and IL6.[Conclusions]Sijicao granules could play an important role for the treatment of eczema through multi-component,multi-target,multi-pathway and their interaction.展开更多
基金funded by the Foundation for University Key Teacher by Henan Educational Committee (2013GGJS070)the National Basic Research Program of China (2014CB954202)+1 种基金the National Natural Science Foundation of China (40971150)the China Scholarship Council (201208410020)
文摘Desert ephemeral plants play an important role in desert ecosystem.Soil water availability is considered as the major restrictive factor limiting the growth of ephemeral plants.Moreover,arbuscular mycorrhizal fungi（AM fungi） are widely reported to improve the growth of desert ephemerals.The present study aimed to test the hypothesis of that AM fungi could alleviate drought stress of desert ephemeral Plantago minuta,and AM fungal functions reduced with the improvement of soil water content.A pot experiment was carried out with three levels of soil water contents（4.5%,9.0%,and 15.8%（w/w））,and three AM inoculation treatments（Glomus mosseae,Glomus etunicatum and non-inoculation）.The results indicate that mycorrhizal colonization rate decreased with the increase of soil water availability.Inoculation improved plant growth and N,P and K acquisition in both shoots and roots regardless water treatments.When comparing the two fungi,plants inoculated with G.mosseae performed better than those inoculated with G.etunicatum in terms of plant growth and nutrient acquisition.These results showed that ameliorative soil water did not suppress arbuscular mycorrhizal fungal functions in improving growth and nutrient acquisition of desert ephemeral Plantago minuta.
基金supported by funding from Jilin Province (No.20060904-1 and 20071102).
文摘Two phenylethanoid glycosides （PhGs） were isolated and purified from the aerial parts of Plantago asiatica for the first time by high performance centrifugal partition chromatography （HPCPC） using ethyl acetate-n-butanol-ethanol-water （0.5：0.5：0.1：1, v/v/v/v）. A total of 45.6 mg of compound 1 and 293.8 mg of compound 2 were purified from 1341 mg of the n-butanol extract of P. asiatica. The structures of the two PhGs were tentatively identified as plantamajoside and aeteoside or isoacteoside by eleetrospray ionization multi stage tandem mass spectrometry （ESI-MS^n） in the negative ion mode.
基金Supported by the National Natural Science Foundation of China(No.30873364)the Natural Science Foundation of Jilin Province of China(Nos.20071102 and 2008-167)
文摘Two phenylethanoid glycosides（PhGs）, plantamajoside and acteoside, were isolated and purified from the aerial parts ofPlantago asiatica for the first time by high performance centrifugal partition chromatography（HPCPC） with ethyl acetate, n-butanol, ethanol and water（0.5：0.5：0.1：1, volume ratio） as solvent system. A total of 45.6 mg of plantamajoside and 293.8 mg of acteoside were purified from 1341 mg of the n-butanol extract of P. asiatica, with a purity of 〉93.3% as determined by HPLC. The HPCPC fractions were analyzed by HPLC-DAD and the structures were identified by their retention time, UV, electrospray ionization multi stage tandem mass spectrometry（ESI-MSn） in the negative ion mode, and confirmed by NMR experiments. The characteristic fragment ions of ESI-MS of the two PhGs isolated from Plantago asiatica were discussed, which are specific and useful for the identification of the structures of PhGs.
文摘本研究采用响应面设计优化超声辅助提取车前总黄酮的最佳条件,然后用此条件提取大车前和平车前总黄酮,并探究大孔树脂纯化对三种车前草总黄酮抗氧化活性的影响。结果表明,在超声温度60℃、乙醇浓度70%的条件下,车前总黄酮最佳提取工艺参数为液料比20:1 m L/g、超声时间80 min、超声功率210 W,车前、大车前和平车前的总黄酮得率分别为5.04%、2.86%和1.22%。无论是纯化前还是纯化后,大车前总黄酮的还原力和对羟基自由基的清除作用最强,平车前最弱;车前总黄酮对DPPH自由基的清除作用最大,平车前最弱。纯化前后的还原力和对DPPH自由基、羟基自由基的清除作用都接近Vc的水平。
基金supported financially by the grants from the Chinese Academy of Sciences(KSCX2-EW-Q-10 and KSCX1-YW-R-24)the NSFC(No.20802082 and 30830115)+1 种基金the Major State Basic Research Development Program of China(No.2009CB522303 and 2009CB940900)the project of recruited top talent of sciences and technology of Yunnan Province(2006PY01-47).
文摘Four new indole alkaloids,plasiaticines A-D(1-4),together with two known ones,were isolated from the seeds of Plantago asiatica.The structures of the new compounds were elucidated on the basis of comprehensive analysis of spectroscopic data.All compounds were tested for their cytotoxic activity,and all compounds except 4 were tested for their acetylcholinesterase(AChE)inhibitory activities.
基金Supported by Science and Technology Planning Project of Guizhou Province[QKHJC-ZK(2022)362]Science and Technology Achievements Transfer and Transformation Project of Guizhou Provincial Department of Education[(2022)064]+1 种基金Higher Education Institution Engineering Research Center of Guizhou Provincial Department of Education[(2023)035,No.(2023)zk01]National Undergraduate Innovation Training Project(202210660131,202310660082).
文摘[Objectives]To explore the pharmacodynamic material basis of Sijicao granules for the treatment of eczema through chemical composition-network pharmacology.[Methods]First of all,the chemical constituents of Polygonum capitatum and Plantago asiatica from Sijicao granules were collected,and the relevant target information of the constituents was collected by TCMSP,PubChem,DisGeNET,GeneCards and STRING databases.Furthermore,Cytoscape 3.8.2 software was used to construct the chemical compounds-target network map of Sijicao granules.Finally,STRING database was used for PPI protein network analysis,GO functional enrichment analysis and KEGG pathway enrichment analysis of core targets,and molecular docking between core constituents and protein targets was also performed.[Results]30 constituents,including quercetin,kaempferol,luteolin,ellagic acid and gallic acid,were discovered to be the key effective compounds of Sijicao granules in the treatment of eczema.And its core action protein targets were PTGS2,NOS2,AKT1,TP53,IL6,HMOX1.What s more,through GO functional enrichment analysis of biological process(BP),cell component(CC),molecular function(MF)analysis and KEGG pathway enrichment analysis,the main pathways of action of Sijicao granules for the treatment of eczema including IL-17 signaling pathway,T cell receptor signaling pathway,cancer signaling pathway,TNF signaling pathway and Relaxin signaling pathway.In addition,molecular docking results displayed that the primary active constituents quercetin,kaempferol and luteolin were well combined with the core protein targets AKT1 and IL6.[Conclusions]Sijicao granules could play an important role for the treatment of eczema through multi-component,multi-target,multi-pathway and their interaction.
