以3年生的银杏幼树为实验材料,经过淹水处理后,测定不同时间银杏叶片内主要生化指标——丙二醛(M DA)、游离脯氨酸(PRO)、可溶性糖以及超氧化物歧化酶(SOD)含量的变化.结果表明:淹水后的前9天,银杏叶片丙二醛含量无明显变化,第10天,其M...以3年生的银杏幼树为实验材料,经过淹水处理后,测定不同时间银杏叶片内主要生化指标——丙二醛(M DA)、游离脯氨酸(PRO)、可溶性糖以及超氧化物歧化酶(SOD)含量的变化.结果表明:淹水后的前9天,银杏叶片丙二醛含量无明显变化,第10天,其M DA含量显著增加,达到18.7μm o l/g,比对照M DA含量(7.2μm o l/g)增加1倍多;淹水后第8天,银杏叶片游离脯氨酸(PRO)含量显著增高,达到101.4μg/g,为对照的2倍多;随着淹水时间的延长,银杏叶片可溶性糖的含量逐渐增加,第9天达到32.9 m g/g,随后维持在较高水平上;在淹水后前几天,SOD的活性增加较快,第5天,SOD活性达到最高值854.6 U/g,第6天开始下降,到第11天时,其活性为78.8 U/g,远远低于对照组.展开更多
Objective:Our aim was to prospectively compare the Accuracy of Acute Physiology and Chronic Health Evaluation(APACHE)II,Bedside Index of Severity in Acute Pancreatitis(BISAP),Ranson’s score and modified Computed Tomo...Objective:Our aim was to prospectively compare the Accuracy of Acute Physiology and Chronic Health Evaluation(APACHE)II,Bedside Index of Severity in Acute Pancreatitis(BISAP),Ranson’s score and modified Computed Tomography Severity Index(CTSI)in predicting the severity of acute pancreatitis based on Atlanta 2012 definitions in a tertiary care hospital in northern India.Methods:Fifty patients with acute pancreatitis admitted to our hospital during the period of March 2015 to September 2016 were included in the study.APACHE II,BISAP and Ranson’s score were calculated for all the cases.Modified CTSI was also determined based on a pancreatic protocol contrast enhanced computerized tomography(CT).Optimal cut-offs for these scoring systems and the area under the curve(AUC)were evaluated based on the receiver operating characteristics(ROC)curve and these scoring systems were compared prospectively.Results:Of the 50 cases,14 were graded as severe acute pancreatitis.Pancreatic necrosis was present in 15 patients,while 14 developed persistent organ failure and 14 needed intensive care unit(ICU)admission.The AUC for modified CTSI was consistently the highest for predicting severe acute pancreatitis(0.919),pancreatic necrosis(0.993),organ failure(0.893)and ICU admission(0.993).APACHE II was the second most accurate in predicting severe acute pancreatitis(AUC 0.834)and organ failure(0.831).APACHE II had a high sensitivity for predicting pancreatic necrosis(93.33%),organ failure(92.86%)and ICU admission(92.31%),and also had a high negative predictive value for predicting pancreatic necrosis(96.15%),organ failure(96.15%)and ICU admission(95.83%).Conclusion:APACHE II is a useful prognostic scoring system for predicting the severity of acute pancreatitis and can be a crucial aid in determining the group of patients that have a high chance of need for tertiary care during the course of their illness and therefore need early resuscitation and prompt referral,especially in resource-limited developing countries.展开更多
文摘以3年生的银杏幼树为实验材料,经过淹水处理后,测定不同时间银杏叶片内主要生化指标——丙二醛(M DA)、游离脯氨酸(PRO)、可溶性糖以及超氧化物歧化酶(SOD)含量的变化.结果表明:淹水后的前9天,银杏叶片丙二醛含量无明显变化,第10天,其M DA含量显著增加,达到18.7μm o l/g,比对照M DA含量(7.2μm o l/g)增加1倍多;淹水后第8天,银杏叶片游离脯氨酸(PRO)含量显著增高,达到101.4μg/g,为对照的2倍多;随着淹水时间的延长,银杏叶片可溶性糖的含量逐渐增加,第9天达到32.9 m g/g,随后维持在较高水平上;在淹水后前几天,SOD的活性增加较快,第5天,SOD活性达到最高值854.6 U/g,第6天开始下降,到第11天时,其活性为78.8 U/g,远远低于对照组.
文摘Objective:Our aim was to prospectively compare the Accuracy of Acute Physiology and Chronic Health Evaluation(APACHE)II,Bedside Index of Severity in Acute Pancreatitis(BISAP),Ranson’s score and modified Computed Tomography Severity Index(CTSI)in predicting the severity of acute pancreatitis based on Atlanta 2012 definitions in a tertiary care hospital in northern India.Methods:Fifty patients with acute pancreatitis admitted to our hospital during the period of March 2015 to September 2016 were included in the study.APACHE II,BISAP and Ranson’s score were calculated for all the cases.Modified CTSI was also determined based on a pancreatic protocol contrast enhanced computerized tomography(CT).Optimal cut-offs for these scoring systems and the area under the curve(AUC)were evaluated based on the receiver operating characteristics(ROC)curve and these scoring systems were compared prospectively.Results:Of the 50 cases,14 were graded as severe acute pancreatitis.Pancreatic necrosis was present in 15 patients,while 14 developed persistent organ failure and 14 needed intensive care unit(ICU)admission.The AUC for modified CTSI was consistently the highest for predicting severe acute pancreatitis(0.919),pancreatic necrosis(0.993),organ failure(0.893)and ICU admission(0.993).APACHE II was the second most accurate in predicting severe acute pancreatitis(AUC 0.834)and organ failure(0.831).APACHE II had a high sensitivity for predicting pancreatic necrosis(93.33%),organ failure(92.86%)and ICU admission(92.31%),and also had a high negative predictive value for predicting pancreatic necrosis(96.15%),organ failure(96.15%)and ICU admission(95.83%).Conclusion:APACHE II is a useful prognostic scoring system for predicting the severity of acute pancreatitis and can be a crucial aid in determining the group of patients that have a high chance of need for tertiary care during the course of their illness and therefore need early resuscitation and prompt referral,especially in resource-limited developing countries.
