The research isolated phosphorus-soluble bacteria from different parts of ryegrasses and selected 7 bacteria performing better in solbilizing capacity. The test results showed that the capacity of phosphorus-solubiliz...The research isolated phosphorus-soluble bacteria from different parts of ryegrasses and selected 7 bacteria performing better in solbilizing capacity. The test results showed that the capacity of phosphorus-solubilizing tended to be volatile in the range from 135.27 to 187.87 μg/ml and the secreting capacity of IAA was in3.47-24.27 μg/ml. It is believed that Lp59, Lp61, Lp65, Lp69, Lp70 and Lp72 are potential for further development.展开更多
[Objective] The study aimed to isolate and screen efficient phosphorus-sol- ubilizing strains from the root soil of alfalfa in Guizhou, and investigate its growth promoting mechanism. [Method] Phosphorus solubilizing ...[Objective] The study aimed to isolate and screen efficient phosphorus-sol- ubilizing strains from the root soil of alfalfa in Guizhou, and investigate its growth promoting mechanism. [Method] Phosphorus solubilizing bacteria were isolated from rhizosphere of alfalfa with inorganic and organic phosphorous medium and the strains with higher capacity of dissolving phosphorus were screened through dissolv- ing phosphate zone and further researched. Meanwhile the phosphorus solubilizing capability of strains cultured in liquid medium was investigated by molybdenum blue colorimetric method. [Result] The capacity of dissolving calcium phosphate of 11 se- lected strains differed significantly and tended to be volatile from 150.40 to 268.20 μg/ml; phosphate solubilization capacity, IAA secretion and the pH of culture media did not show any significant correlation; all strains could produce IAA, and the se- cretion was from 12.09 to 22.16 μg/ml; the selected strains all could produce alka- line matter, and most of colonies were pale or milky white, irregular, opaque, jagged, flat and non-pigmented; utilization of carbon sources by different strains varied signifi- cantly. [Conclusion] The study laid the foundation for relieving phosphorus in unpro- ductive land, saving phosphate resources, reducing environmental pollution and pro- viding fertilizer in alfalfa production.展开更多
【目的】从磷矿区植物根围土中,筛选出具有较高溶磷能力的菌株及优化溶磷培养条件.【方法】通过稀释凃板法分离、筛选菌株,并进行全细胞脂肪酸分析和16S r DNA测序鉴定;正交试验优化溶磷培养条件.【结果和结论】筛选出22株具有溶磷能力...【目的】从磷矿区植物根围土中,筛选出具有较高溶磷能力的菌株及优化溶磷培养条件.【方法】通过稀释凃板法分离、筛选菌株,并进行全细胞脂肪酸分析和16S r DNA测序鉴定;正交试验优化溶磷培养条件.【结果和结论】筛选出22株具有溶磷能力的菌株,其中菌株YN2014102的溶磷能力最强,在10 g·L-1的磷矿粉培养基中能释放244.75 mg·L-1水溶性磷.经全细胞脂肪酸分析和16S r DNA测序,该菌株被鉴定为洋葱伯克霍尔德菌.正交试验的结果表明,在质量浓度10 g·L-1的磷矿粉、28℃、摇床转速为180 r·min-1的条件下培养5 d,溶磷效果最好,达277.08 mg·L-1.展开更多
基金Supported by Social Development Projects in Guizhou Province(NY[2011]3104)System Reform Program of Guizhou Province(Z[2012]4005)~~
文摘The research isolated phosphorus-soluble bacteria from different parts of ryegrasses and selected 7 bacteria performing better in solbilizing capacity. The test results showed that the capacity of phosphorus-solubilizing tended to be volatile in the range from 135.27 to 187.87 μg/ml and the secreting capacity of IAA was in3.47-24.27 μg/ml. It is believed that Lp59, Lp61, Lp65, Lp69, Lp70 and Lp72 are potential for further development.
基金Supported by Key Agricultural Program of Guizhou Province([2009]3067)Science and Technology Foundation of Guizhou Province([2012]2199)~~
文摘[Objective] The study aimed to isolate and screen efficient phosphorus-sol- ubilizing strains from the root soil of alfalfa in Guizhou, and investigate its growth promoting mechanism. [Method] Phosphorus solubilizing bacteria were isolated from rhizosphere of alfalfa with inorganic and organic phosphorous medium and the strains with higher capacity of dissolving phosphorus were screened through dissolv- ing phosphate zone and further researched. Meanwhile the phosphorus solubilizing capability of strains cultured in liquid medium was investigated by molybdenum blue colorimetric method. [Result] The capacity of dissolving calcium phosphate of 11 se- lected strains differed significantly and tended to be volatile from 150.40 to 268.20 μg/ml; phosphate solubilization capacity, IAA secretion and the pH of culture media did not show any significant correlation; all strains could produce IAA, and the se- cretion was from 12.09 to 22.16 μg/ml; the selected strains all could produce alka- line matter, and most of colonies were pale or milky white, irregular, opaque, jagged, flat and non-pigmented; utilization of carbon sources by different strains varied signifi- cantly. [Conclusion] The study laid the foundation for relieving phosphorus in unpro- ductive land, saving phosphate resources, reducing environmental pollution and pro- viding fertilizer in alfalfa production.
