In order to investigate the competition for carbon source between denitrification and phosphorus release processes,simultaneous phosphorus release and denitrification in sludge operated in anoxic,aerobic mode are inve...In order to investigate the competition for carbon source between denitrification and phosphorus release processes,simultaneous phosphorus release and denitrification in sludge operated in anoxic,aerobic mode are investigated by varying the ratio of influent COD to nitrogenous compound concentration under anoxic condition using a lab-scale sequencing batch reactor（SBR）.The results show that the nitrate reduction rate is nearly independent of the ratio of influent COD to nitrate under anoxic condition.More NOx^--N in the influent leads to less PO4^3--P release during the feeding period.However, PO4^3--P release proceeds at a low rate simultaneously with denitrification even when the influent NOx^--N concentration is as large as 20 mg·L^-1and its rate is increased obviously when NOx^--N is denitrified to a concentration lower than 0.5 mg·L^-1.The variation of pH during anoxic period gives some information about the biochemical reactions of denitrification and PO4^3--P release.When more nitrate is present in the influent,more acetate uptake in feeding period is used for direct microorganism growth.展开更多
Periphytic biofilms exist widely in paddy fields, but their influences on the hydrolysis of organic phosphorus(P) have rarely been investigated. In this study,a periphytic biofilm was incubated in a paddy soil solutio...Periphytic biofilms exist widely in paddy fields, but their influences on the hydrolysis of organic phosphorus(P) have rarely been investigated. In this study,a periphytic biofilm was incubated in a paddy soil solution, and hydrolysis kinetic parameters(half-saturation constant(Km) and maximum catalytic reaction rate(Vmax)), optimal environmental conditions, substrate specificity, and response to different P regimes of the phosphatase activities in the periphytic biofilm were determined, in order to characterize extracellular phosphatase activities in periphytic biofilms from paddy fields. The results indicated that the periphytic biofilm could produce an acid phosphomonoesterase(PMEase), an alkaline PMEases, and a phosphodiesterase(PDEase). These three phosphatases displayed high substrate affinity, with Km values ranging from 141.03 to 212.96 μmol L^(-1). The Vmax/Km ratios for the phosphatases followed the order of alkaline PMEase > acid PMEase > PDEase, which suggested that the PMEases, especially the alkaline PMEase, had higher catalytic efficiency. The optimal pH was 6.0 for the acid PMEase activity and 8.0 for the PDEase activity, and the alkaline PMEase activity increased with a pH increase from 7.0 to 12.0. The optimal temperature was 50℃ for the PMEases and 60℃ for the PDEase. The phosphatases showed high catalytic efficiency for condensed P over a wide pH range and for orthophosphate monoesters at pH 11.0, except for inositol hexakisphosphate at pH 6.0. The inorganic P supply was the main factor in the regulation of phosphatase activities. These findings demonstrated that the periphytic biofilm tested had high hydrolysis capacity for organic and condensed P,especially under P-limited conditions.展开更多
[ Objective] The study aimed to discuss the removal rate of phosphorus by different constructed wetland substrates. [ Methed] Based on static experiments, we analyzed the removal rate and characteristics of phosphorus...[ Objective] The study aimed to discuss the removal rate of phosphorus by different constructed wetland substrates. [ Methed] Based on static experiments, we analyzed the removal rate and characteristics of phosphorus by different constructed wetland substrates like steel slag, cin- der slag, shale, boiler slag, soil and gravel. [ Result~ The maximum adsorption of phosphorus by various substrates showed as follows, steel slag 〉 cinder slag 〉 shale 〉 boiler slag 〉 soil 〉 gravel. When the initial concentration of phosphorus was 5 mg/L, the removal rate of phosphorus by the steel slag, cinder slag, shale, boiler slag, soil and gravel was 99.76%, 85.8%, 71.2%, 63.0%, 46.8% and 11.7% respectively. It is suggested that shale and boiler slag can be used as the substrate of subsurface-flow constructed wetlands; cinder slag can be chosen as a renewable sub- strate for intensifying phosphorus removal; steel slag could be used at the end of subsurface-flow constructed wetlands for treating TP in outflows. [Concluslon] The research could provide theoretical references for choosing a suitable substrate for constructed wetlands to remove phosphorus in future.展开更多
[Objective] The aim was to find an efficient method to measure available nitrogen, phosphorus and potassium content in vegetable seedling substrate. [ Method] The suitable dosage of leaching agent, ratio of the substr...[Objective] The aim was to find an efficient method to measure available nitrogen, phosphorus and potassium content in vegetable seedling substrate. [ Method] The suitable dosage of leaching agent, ratio of the substrate to water and leaching time for the detection of nutrient contents in the substrate by a soil nutrient measuring instrument were discussed firstly, and then the results of nutrient contents measured by the soil nutrient measuring instrument were compared with that by conventional approaches. [ Result] In compadson with the conventional methods, the av- erage content of available nitrogen measured by the soil nutrient measuring instrument was slightly higher, while the average content of available phosphorus measured by the instrument was lower; the average content of available potassium measured by the instrument was close to that by the conventional method. [ Conclusion] This study could provide us a new method to detect available nitrogen, phosphorus and potassium content in seedling substrate efficiently.展开更多
基金Supported by the Scientific Innovation Platform of Beijing Municipal Education Commission(PXM2008_014204_050843)State Key Laboratory of Urban Water Resource and Environment(HIT)(QAK200802)Environment and Water Industry(EWI)Development Scheme of Singapore-The Process Control and Enhanced Biological Nutrient Removal of CSTR Proc-ess(EDB S07/1-53974082)
文摘In order to investigate the competition for carbon source between denitrification and phosphorus release processes,simultaneous phosphorus release and denitrification in sludge operated in anoxic,aerobic mode are investigated by varying the ratio of influent COD to nitrogenous compound concentration under anoxic condition using a lab-scale sequencing batch reactor（SBR）.The results show that the nitrate reduction rate is nearly independent of the ratio of influent COD to nitrate under anoxic condition.More NOx^--N in the influent leads to less PO4^3--P release during the feeding period.However, PO4^3--P release proceeds at a low rate simultaneously with denitrification even when the influent NOx^--N concentration is as large as 20 mg·L^-1and its rate is increased obviously when NOx^--N is denitrified to a concentration lower than 0.5 mg·L^-1.The variation of pH during anoxic period gives some information about the biochemical reactions of denitrification and PO4^3--P release.When more nitrate is present in the influent,more acetate uptake in feeding period is used for direct microorganism growth.
基金supported by the State Key Basic Research Program of China (No. 2015CB158200)the National Natural Science Foundation of China (No. 41877102)+1 种基金the Interdisciplinary Innovation Team of Chinese Academy of Sciences (CAS)the Youth Innovation Promotion Association of CAS (No. 2014272)。
文摘Periphytic biofilms exist widely in paddy fields, but their influences on the hydrolysis of organic phosphorus(P) have rarely been investigated. In this study,a periphytic biofilm was incubated in a paddy soil solution, and hydrolysis kinetic parameters(half-saturation constant(Km) and maximum catalytic reaction rate(Vmax)), optimal environmental conditions, substrate specificity, and response to different P regimes of the phosphatase activities in the periphytic biofilm were determined, in order to characterize extracellular phosphatase activities in periphytic biofilms from paddy fields. The results indicated that the periphytic biofilm could produce an acid phosphomonoesterase(PMEase), an alkaline PMEases, and a phosphodiesterase(PDEase). These three phosphatases displayed high substrate affinity, with Km values ranging from 141.03 to 212.96 μmol L^(-1). The Vmax/Km ratios for the phosphatases followed the order of alkaline PMEase > acid PMEase > PDEase, which suggested that the PMEases, especially the alkaline PMEase, had higher catalytic efficiency. The optimal pH was 6.0 for the acid PMEase activity and 8.0 for the PDEase activity, and the alkaline PMEase activity increased with a pH increase from 7.0 to 12.0. The optimal temperature was 50℃ for the PMEases and 60℃ for the PDEase. The phosphatases showed high catalytic efficiency for condensed P over a wide pH range and for orthophosphate monoesters at pH 11.0, except for inositol hexakisphosphate at pH 6.0. The inorganic P supply was the main factor in the regulation of phosphatase activities. These findings demonstrated that the periphytic biofilm tested had high hydrolysis capacity for organic and condensed P,especially under P-limited conditions.
基金Supported by National Natural Science Foundation of China(50278016)
文摘[ Objective] The study aimed to discuss the removal rate of phosphorus by different constructed wetland substrates. [ Methed] Based on static experiments, we analyzed the removal rate and characteristics of phosphorus by different constructed wetland substrates like steel slag, cin- der slag, shale, boiler slag, soil and gravel. [ Result~ The maximum adsorption of phosphorus by various substrates showed as follows, steel slag 〉 cinder slag 〉 shale 〉 boiler slag 〉 soil 〉 gravel. When the initial concentration of phosphorus was 5 mg/L, the removal rate of phosphorus by the steel slag, cinder slag, shale, boiler slag, soil and gravel was 99.76%, 85.8%, 71.2%, 63.0%, 46.8% and 11.7% respectively. It is suggested that shale and boiler slag can be used as the substrate of subsurface-flow constructed wetlands; cinder slag can be chosen as a renewable sub- strate for intensifying phosphorus removal; steel slag could be used at the end of subsurface-flow constructed wetlands for treating TP in outflows. [Concluslon] The research could provide theoretical references for choosing a suitable substrate for constructed wetlands to remove phosphorus in future.
基金Supported by the Scientific Research Project of Public Welfare Industry(Agriculture)(201303014-01)Science and Technology Innovation Team of Anhui Academy of Agricultural Sciences(14C0314)the Dean’s Youth Innovation Fund from Anhui Academy of Agricultural Sciences(15B0331)
文摘[Objective] The aim was to find an efficient method to measure available nitrogen, phosphorus and potassium content in vegetable seedling substrate. [ Method] The suitable dosage of leaching agent, ratio of the substrate to water and leaching time for the detection of nutrient contents in the substrate by a soil nutrient measuring instrument were discussed firstly, and then the results of nutrient contents measured by the soil nutrient measuring instrument were compared with that by conventional approaches. [ Result] In compadson with the conventional methods, the av- erage content of available nitrogen measured by the soil nutrient measuring instrument was slightly higher, while the average content of available phosphorus measured by the instrument was lower; the average content of available potassium measured by the instrument was close to that by the conventional method. [ Conclusion] This study could provide us a new method to detect available nitrogen, phosphorus and potassium content in seedling substrate efficiently.
