Phosphotidylinositol (PI) can be hydrolyzed into inositol 1,4,5_ trisphosphate/diacylglycerol (IP 3/DAG), the second signalling messengers which can be further phosphorylated by phospholipases to other signalling mole...Phosphotidylinositol (PI) can be hydrolyzed into inositol 1,4,5_ trisphosphate/diacylglycerol (IP 3/DAG), the second signalling messengers which can be further phosphorylated by phospholipases to other signalling molecules, and phosphatidic acid (PA) and lysophosphoglyceride (lysoPI) involved in many signalling cascades. It has been shown that PI signalling pathway and the involved enzymes are critical for plant development and response to environmental factors. Three classes of phospholipases, named PLA 2, PLC and PLD, were classified based on their cleavage sites, and the presence of variants of isoforms indicating their different functions in plant growth/development. The update information of phospholipases with focus on the interaction of plant hormone and environmental factors has been provided.展开更多
1 材料和方法1982-10/2000-02我院收治急性重症胰腺炎86例,手术治疗61例(72%),非手术治疗25例(29%),发生各种并发症65例(75%),死亡19例(病死率为21%),其中胰性脑病13例(发生率为15%),死于胰性脑病6例(46%).男6例,女7例,年龄31岁～82岁(...1 材料和方法1982-10/2000-02我院收治急性重症胰腺炎86例,手术治疗61例(72%),非手术治疗25例(29%),发生各种并发症65例(75%),死亡19例(病死率为21%),其中胰性脑病13例(发生率为15%),死于胰性脑病6例(46%).男6例,女7例,年龄31岁～82岁(平均47.4岁).本组13例胰性脑病中10例施行手求治疗,3例未手术.13例胰性脑病死亡6例,病死率为46%.本组胰性脑病从急性重症胰腺炎起病至病后57 d 均有发生,手术前后均有胰性脑病发生,胰性脑病多在术后36展开更多
Objective: To study the effect of polydatin on p hospholipase A2 in lung tissues in rats with endotoxic shock. Methods: Thirty-two healthy male Wistar rats were employed in this study. A total of 8 rats received norma...Objective: To study the effect of polydatin on p hospholipase A2 in lung tissues in rats with endotoxic shock. Methods: Thirty-two healthy male Wistar rats were employed in this study. A total of 8 rats received normal saline intravenously （control grou p）,8 rats received 10 mg/kg of endotoxin （endotoxic shock group）,8 rats re ceived 1 mg/kg of polydatin after endotoxin injection （polydatin treatment g roup）,and 8 rats received 1 mg/kg of polydatin （polydatin prevention group） 30 minutes before endotoxin injection. Mean arterial pressure was measured once hal f an hour. Lung tissues were collected 6 hours later. Phospholipase A2 activit y was measured with acid titration. The gene expression of secretory phospholipa se A2 type IIA was detected with reverse transcription polymerase chain reacti on. Meanwhile,the histological changes of the lungs among four groups were comp ared through microscopic examination.Results: Phospholipase A2 activity and the gene expression of secretory phospholipase A2 type IIA increased after endotoxin injection,but polydatin could inhibit these effects of endotoxin. Obvious morphological eviden ce could be found in the lung pathological sections and the protective effect of polydatin was most significant in the polydatin prevention group.Conclusions: Polydatin has prophylactic and therapeutic effects （the former is more distinct than the latter） on acutely injured lungs in rats with endotoxic shock and which suggests that polydatin may be a phospholipase A 2 inhibitor.展开更多
文摘Phosphotidylinositol (PI) can be hydrolyzed into inositol 1,4,5_ trisphosphate/diacylglycerol (IP 3/DAG), the second signalling messengers which can be further phosphorylated by phospholipases to other signalling molecules, and phosphatidic acid (PA) and lysophosphoglyceride (lysoPI) involved in many signalling cascades. It has been shown that PI signalling pathway and the involved enzymes are critical for plant development and response to environmental factors. Three classes of phospholipases, named PLA 2, PLC and PLD, were classified based on their cleavage sites, and the presence of variants of isoforms indicating their different functions in plant growth/development. The update information of phospholipases with focus on the interaction of plant hormone and environmental factors has been provided.
文摘1 材料和方法1982-10/2000-02我院收治急性重症胰腺炎86例,手术治疗61例(72%),非手术治疗25例(29%),发生各种并发症65例(75%),死亡19例(病死率为21%),其中胰性脑病13例(发生率为15%),死于胰性脑病6例(46%).男6例,女7例,年龄31岁～82岁(平均47.4岁).本组13例胰性脑病中10例施行手求治疗,3例未手术.13例胰性脑病死亡6例,病死率为46%.本组胰性脑病从急性重症胰腺炎起病至病后57 d 均有发生,手术前后均有胰性脑病发生,胰性脑病多在术后36
文摘Objective: To study the effect of polydatin on p hospholipase A2 in lung tissues in rats with endotoxic shock. Methods: Thirty-two healthy male Wistar rats were employed in this study. A total of 8 rats received normal saline intravenously （control grou p）,8 rats received 10 mg/kg of endotoxin （endotoxic shock group）,8 rats re ceived 1 mg/kg of polydatin after endotoxin injection （polydatin treatment g roup）,and 8 rats received 1 mg/kg of polydatin （polydatin prevention group） 30 minutes before endotoxin injection. Mean arterial pressure was measured once hal f an hour. Lung tissues were collected 6 hours later. Phospholipase A2 activit y was measured with acid titration. The gene expression of secretory phospholipa se A2 type IIA was detected with reverse transcription polymerase chain reacti on. Meanwhile,the histological changes of the lungs among four groups were comp ared through microscopic examination.Results: Phospholipase A2 activity and the gene expression of secretory phospholipase A2 type IIA increased after endotoxin injection,but polydatin could inhibit these effects of endotoxin. Obvious morphological eviden ce could be found in the lung pathological sections and the protective effect of polydatin was most significant in the polydatin prevention group.Conclusions: Polydatin has prophylactic and therapeutic effects （the former is more distinct than the latter） on acutely injured lungs in rats with endotoxic shock and which suggests that polydatin may be a phospholipase A 2 inhibitor.
