AIM： Enterovirus 71 （EV71） has been implicated as the etiological agent responsible for the recent outbreaks of hand, foot and mouth disease associated with severe neurological diseases in the Asia-Pacific region. ...AIM： Enterovirus 71 （EV71） has been implicated as the etiological agent responsible for the recent outbreaks of hand, foot and mouth disease associated with severe neurological diseases in the Asia-Pacific region. METHODS： The assembly process was hypothesized to occur via an orchestrated proteolytic processing of the P1 precursor by the viral protease 3CD. To test this hypothesis, we constructed 3 recombinant baculoviruses： Bac-P1 expressing P1; Bac-3CD expressing 3CD; and Bac-P1-3CD co-expressing P1 and 3CD. RESULTS： Both single infection by Bac-P1-3CD and coinfection by Bac-P1 and Bac-3CD resulted in correct cleavage of P1 to yield individual proteins VP0, VP1 and VP3, while the former approach yielded higher VLP production. In the cells, the structural proteins selfassembled into clusters of virus-like particles （VLP） resembling the authentic EV71 particle aggregates. After ultracentrifugation purification, the dispersed VLPs were indistinguishable from the authentic virus in size, appearance, composition and surface epitopes, as determined by SDS-PAGE, Western blot, transmission electron microscopy and immunogold labeling. CONCLUSION： Our data, for the first time, suggest that in insect cells EV71 structural proteins adopt a processing and assembly pathway similar to poliovirus assembly. The preservation of particle morphology and composition suggest that the VLP may be a valuable vaccine candidate to prevent EV71 epidemics.展开更多
A particle mapping transportation algorithm was proposed on the basis of the particle-in-cell method.The particles with rectangular influence domains were employed in the transportation algorithm to reduce the numeric...A particle mapping transportation algorithm was proposed on the basis of the particle-in-cell method.The particles with rectangular influence domains were employed in the transportation algorithm to reduce the numerical fluctuations.Based on the error analysis in the process of particle motion computation,a prediction-correction algorithm was introduced to improve the computational accuracy.Furthermore,the performance of the particle mapping transportation method was evaluated by using the rotation,the slotted disk and the shear advection tests,and the results were compared with other interface reconstruction methods.Finally,the hemispherical projectile penetration into a steel target was numerically simulated.The results showed that the proposed method produced less numerical fluctuations and exhibited clear material interfaces,which indicated that it is accurate and effective.展开更多
A hybrid VOF and PIC multi-material interface treatment method for Eulerian method is presented in this study in order to solve the problem that the Eulerian method is not robust enough to treat the dynamic fracture o...A hybrid VOF and PIC multi-material interface treatment method for Eulerian method is presented in this study in order to solve the problem that the Eulerian method is not robust enough to treat the dynamic fracture of material. This treatment method is used in the important computational region such as the material interface,large deformation region and fracture region where more particles are added for calculation,while the continuous transport method is used in the other regions. Through this method,a series of penetration problems such as blunt projectile penetration into the steel target and steel projectile penetration into concert target are numerically simulated. The comparison of the numerical result and experimental result shows that the hybrid algorithm can effectively track the deformation in the process of penetration and also insure precision and efficiency.展开更多
基金Supported by the National Science Council,No.93-2214-E-007-016 Ministry of Economic Affairs,No.93-EC-17A-17S1-0009
文摘AIM： Enterovirus 71 （EV71） has been implicated as the etiological agent responsible for the recent outbreaks of hand, foot and mouth disease associated with severe neurological diseases in the Asia-Pacific region. METHODS： The assembly process was hypothesized to occur via an orchestrated proteolytic processing of the P1 precursor by the viral protease 3CD. To test this hypothesis, we constructed 3 recombinant baculoviruses： Bac-P1 expressing P1; Bac-3CD expressing 3CD; and Bac-P1-3CD co-expressing P1 and 3CD. RESULTS： Both single infection by Bac-P1-3CD and coinfection by Bac-P1 and Bac-3CD resulted in correct cleavage of P1 to yield individual proteins VP0, VP1 and VP3, while the former approach yielded higher VLP production. In the cells, the structural proteins selfassembled into clusters of virus-like particles （VLP） resembling the authentic EV71 particle aggregates. After ultracentrifugation purification, the dispersed VLPs were indistinguishable from the authentic virus in size, appearance, composition and surface epitopes, as determined by SDS-PAGE, Western blot, transmission electron microscopy and immunogold labeling. CONCLUSION： Our data, for the first time, suggest that in insect cells EV71 structural proteins adopt a processing and assembly pathway similar to poliovirus assembly. The preservation of particle morphology and composition suggest that the VLP may be a valuable vaccine candidate to prevent EV71 epidemics.
基金supported by the National Basic Research Program of China(Grant No.2010CB832706)the National Natural Science Foundation of China(Grant Nos.11032002 and 11172041)the Project of State Key Laboratory of Explosion Science and Technology(Grant No. ZDKT10-03c)
文摘A particle mapping transportation algorithm was proposed on the basis of the particle-in-cell method.The particles with rectangular influence domains were employed in the transportation algorithm to reduce the numerical fluctuations.Based on the error analysis in the process of particle motion computation,a prediction-correction algorithm was introduced to improve the computational accuracy.Furthermore,the performance of the particle mapping transportation method was evaluated by using the rotation,the slotted disk and the shear advection tests,and the results were compared with other interface reconstruction methods.Finally,the hemispherical projectile penetration into a steel target was numerically simulated.The results showed that the proposed method produced less numerical fluctuations and exhibited clear material interfaces,which indicated that it is accurate and effective.
基金supported by the National Natural Science Foundation of China (Grant Nos. 10625208 and 10872034)the National Basic Research Program of China (Grant No. 2010CB8327006)the Foundation of State Key Laboratory of Explosion Science and Technology (Grant No. ZDKT08-02)
文摘A hybrid VOF and PIC multi-material interface treatment method for Eulerian method is presented in this study in order to solve the problem that the Eulerian method is not robust enough to treat the dynamic fracture of material. This treatment method is used in the important computational region such as the material interface,large deformation region and fracture region where more particles are added for calculation,while the continuous transport method is used in the other regions. Through this method,a series of penetration problems such as blunt projectile penetration into the steel target and steel projectile penetration into concert target are numerically simulated. The comparison of the numerical result and experimental result shows that the hybrid algorithm can effectively track the deformation in the process of penetration and also insure precision and efficiency.
