Aim： To investigate whether chronic bacterial prostatitis might increase oxidative stress and oxidative damage in chronic bacterial prostatitis patients （CBPP）, and to explore its possible mechanism. Methods： Enro...Aim： To investigate whether chronic bacterial prostatitis might increase oxidative stress and oxidative damage in chronic bacterial prostatitis patients （CBPP）, and to explore its possible mechanism. Methods： Enrolled in a casecontrol study were 70 randomly sampled CBPP and 70 randomly sampled healthy adult volunteers （HAV）, on whom plasma nitric oxide （NO）, vitamin C （VC）, vitamin E （VE） and β-carotene （β-CAR） level, erythrocyte malondialdehyde （MDA） level, as well as erythrocyte superoxide dismutase （SOD）, catalase （CAT） and glutathione peroxidase （GPX） activities were determined by spectrophotometry. Results： Compared with the HAV group, values of plasma NO and erythrocyte MDA in the CBPP group were significantly increased （P 〈 0.001）; those of plasma VC, VE and β-CAR as well as erythrocyte SOD, CAT and GPX activities in the CBPP group were significantly decreased （P 〈 0.001）. Findings from partial correlation for the 70 CBPP showed that with prolonged course of disease, values of NO and MDA were gradually increased （P 〈 0.001）, and those of VC, VE, β-CAR, SOD, CAT and GPX were gradually decreased （P 〈 0.05- 0.001）. The findings from stepwise regression for the 70 CBPP suggested that the model was Y= -13.2077 ＋ 0.1894MDA ＋ 0.0415NO - 0.1999GPX, F = 18.2047, P 〈 0.001, r = 0.6729, P 〈 0.001. Conclusion： The findings suggest that there exist increased oxidative stress and oxidative damage induced by chronic bacterial prostatitis in the patients, and such phenomenon was closely related to the course of disease.展开更多
The research addresses the identification of a screening methodology for salt stress tolerance in radish cultivars. In the first experiment, two different radish cultivars(long white and round red) were compared in th...The research addresses the identification of a screening methodology for salt stress tolerance in radish cultivars. In the first experiment, two different radish cultivars(long white and round red) were compared in their morphological and physiological responses to different salinity levels. Round red radish showed better morphological and physiological responses to incremental salinity in terms of yield and better adaptation of overall water relations. In the second experiment, the most tolerant genotype from the first experiment was used as a control against other seven round red radish genotypes ranked by their salinity tolerance according to morphological, physiological and biochemical indices. Salt stress did not significantly affect malondialdehyde(MDA) and hydrogen peroxide(H2O2) content, and ascorbate peroxidase(APX) activity in the studied cultivars. Nonetheless, the relatively salt tolerant cultivar SAXA2 showed higher ability to accumulate compatible solutes(e.g.proline and proteins) and maintain osmotic adjustment. In addition, cultivar SAXA2 also showed considerable increase in glutathione reductase(GR) activity. Our results supported that accumulation of proline and higher GR activity are associated with radish salt tolerance, whereas no relationship with salinity was observed in superoxide dismutase(SOD), MDA and H2O2 content.展开更多
文摘Aim： To investigate whether chronic bacterial prostatitis might increase oxidative stress and oxidative damage in chronic bacterial prostatitis patients （CBPP）, and to explore its possible mechanism. Methods： Enrolled in a casecontrol study were 70 randomly sampled CBPP and 70 randomly sampled healthy adult volunteers （HAV）, on whom plasma nitric oxide （NO）, vitamin C （VC）, vitamin E （VE） and β-carotene （β-CAR） level, erythrocyte malondialdehyde （MDA） level, as well as erythrocyte superoxide dismutase （SOD）, catalase （CAT） and glutathione peroxidase （GPX） activities were determined by spectrophotometry. Results： Compared with the HAV group, values of plasma NO and erythrocyte MDA in the CBPP group were significantly increased （P 〈 0.001）; those of plasma VC, VE and β-CAR as well as erythrocyte SOD, CAT and GPX activities in the CBPP group were significantly decreased （P 〈 0.001）. Findings from partial correlation for the 70 CBPP showed that with prolonged course of disease, values of NO and MDA were gradually increased （P 〈 0.001）, and those of VC, VE, β-CAR, SOD, CAT and GPX were gradually decreased （P 〈 0.05- 0.001）. The findings from stepwise regression for the 70 CBPP suggested that the model was Y= -13.2077 ＋ 0.1894MDA ＋ 0.0415NO - 0.1999GPX, F = 18.2047, P 〈 0.001, r = 0.6729, P 〈 0.001. Conclusion： The findings suggest that there exist increased oxidative stress and oxidative damage induced by chronic bacterial prostatitis in the patients, and such phenomenon was closely related to the course of disease.
文摘The research addresses the identification of a screening methodology for salt stress tolerance in radish cultivars. In the first experiment, two different radish cultivars(long white and round red) were compared in their morphological and physiological responses to different salinity levels. Round red radish showed better morphological and physiological responses to incremental salinity in terms of yield and better adaptation of overall water relations. In the second experiment, the most tolerant genotype from the first experiment was used as a control against other seven round red radish genotypes ranked by their salinity tolerance according to morphological, physiological and biochemical indices. Salt stress did not significantly affect malondialdehyde(MDA) and hydrogen peroxide(H2O2) content, and ascorbate peroxidase(APX) activity in the studied cultivars. Nonetheless, the relatively salt tolerant cultivar SAXA2 showed higher ability to accumulate compatible solutes(e.g.proline and proteins) and maintain osmotic adjustment. In addition, cultivar SAXA2 also showed considerable increase in glutathione reductase(GR) activity. Our results supported that accumulation of proline and higher GR activity are associated with radish salt tolerance, whereas no relationship with salinity was observed in superoxide dismutase(SOD), MDA and H2O2 content.
