With the advent of the era of organ donation after citizen's death,split liver transplantation(SLT)can effectively increase the supply of donor livers and shorten the transplantation wait time for patients,especia...With the advent of the era of organ donation after citizen's death,split liver transplantation(SLT)can effectively increase the supply of donor livers and shorten the transplantation wait time for patients,especially pediatric recipients.In recent years,SLT has been performed to varying degrees in many transplant centers in China,and varying levels of efficacy have been achieved.The quality of donors and donor livers for SLT is an important factor affecting the outcome of the surgery.At this stage,it is necessary to reach a consensus on the evaluation of SLT donor and liver donor that is suitable for the conditions in China by combining the well-established experience of the international community with advanced transplantation technology.This approach will aid in enhancing the efficacy of SLT.Based on the experience of experts,a consensus on the evaluation of donors and donor livers for SLT has been formulated in this study,which focuses on evaluation of donors and donor livers,evaluating the functional and anatomical aspects of the donor liver and donorerecipient matching.展开更多
Objective To evaluate the sensitivity of arterial ketone body ratio as an indicator for multiple organ failure.Materials and methods The experimental model of multiple organ failure was made in adult and old rats by h...Objective To evaluate the sensitivity of arterial ketone body ratio as an indicator for multiple organ failure.Materials and methods The experimental model of multiple organ failure was made in adult and old rats by hypoperfusion-induced hemorrhagic shock. After blood sampling, the arterial acetoacetate, β-hydroxybutyrate, total ketone body, ALT, AST, BUN, creatinine at 2, 4, 8 hr in hypoperfusion were examined to compare the differences of ketone body ratio and organ failure between adult and old rats. Hepatic and mitochondrial metabolism were assessed by comparing ketone body ratios (AcAc/β-OHB) and free NAD+/NADH ratios. Results Ketone body ratio in old rats at 2, 4, 8 hr after the induction of hemorrhagic shock decreased from 0.68 to 0.31, 0.27 and 0.22, respectively. In adult rats, it decreased from 1.12 to 0.17, 0.12 and 0.09, respectively. Changes of ketone body ratio in the adult group were larger than in the elderly group ( P ＜ 0.001). The development of multiple organ failure is associated with the time of hemorrhagic shock development. Conclusions There was a different ketone body ratio between multiple organ failure in the elderly (MOFE) and multiple organ failure (MOF) in general adults. Ketone body ratio is a better indicator than ALT and AST in reflecting hepatic function in the early status of MOF. (J Geriatr Cardiol 2004;1(2) :125-128. )展开更多
基金grants from National 13th Five-Year Science and Technology Plan Major Projects of China(2017ZX10203205)National Key Research and Development Program of China(2017YFA0104304)+3 种基金National Natural Science Foundation of China(81770648,81972286)Natural Science Foundation of Guangdong Province,China(2015A030312013,2020A1515010574,2020A1515010302)Science and Technology Program of Guangdong Province,China(2017B020209004,2019B020236003,2020B1212060019)and Guangzhou Science and Technology Project,China(201803040005).
文摘With the advent of the era of organ donation after citizen's death,split liver transplantation(SLT)can effectively increase the supply of donor livers and shorten the transplantation wait time for patients,especially pediatric recipients.In recent years,SLT has been performed to varying degrees in many transplant centers in China,and varying levels of efficacy have been achieved.The quality of donors and donor livers for SLT is an important factor affecting the outcome of the surgery.At this stage,it is necessary to reach a consensus on the evaluation of SLT donor and liver donor that is suitable for the conditions in China by combining the well-established experience of the international community with advanced transplantation technology.This approach will aid in enhancing the efficacy of SLT.Based on the experience of experts,a consensus on the evaluation of donors and donor livers for SLT has been formulated in this study,which focuses on evaluation of donors and donor livers,evaluating the functional and anatomical aspects of the donor liver and donorerecipient matching.
文摘Objective To evaluate the sensitivity of arterial ketone body ratio as an indicator for multiple organ failure.Materials and methods The experimental model of multiple organ failure was made in adult and old rats by hypoperfusion-induced hemorrhagic shock. After blood sampling, the arterial acetoacetate, β-hydroxybutyrate, total ketone body, ALT, AST, BUN, creatinine at 2, 4, 8 hr in hypoperfusion were examined to compare the differences of ketone body ratio and organ failure between adult and old rats. Hepatic and mitochondrial metabolism were assessed by comparing ketone body ratios (AcAc/β-OHB) and free NAD+/NADH ratios. Results Ketone body ratio in old rats at 2, 4, 8 hr after the induction of hemorrhagic shock decreased from 0.68 to 0.31, 0.27 and 0.22, respectively. In adult rats, it decreased from 1.12 to 0.17, 0.12 and 0.09, respectively. Changes of ketone body ratio in the adult group were larger than in the elderly group ( P ＜ 0.001). The development of multiple organ failure is associated with the time of hemorrhagic shock development. Conclusions There was a different ketone body ratio between multiple organ failure in the elderly (MOFE) and multiple organ failure (MOF) in general adults. Ketone body ratio is a better indicator than ALT and AST in reflecting hepatic function in the early status of MOF. (J Geriatr Cardiol 2004;1(2) :125-128. )
