Olfactory respones of Tetranychus viennensis to different plants odors with “Y” olfactometer and petri-dish was studied and volatiles from leaves of different plants were absorbed with SPME and analyzed with GC-MS. ...Olfactory respones of Tetranychus viennensis to different plants odors with “Y” olfactometer and petri-dish was studied and volatiles from leaves of different plants were absorbed with SPME and analyzed with GC-MS. It was showed: Olfaction takes part in the response during the Hawthorn Spider Mite looking for host plants, indicating %Tetranychus viennensis% were attracted by odors from host plants. Different host plants have different attraction to the %Tetranychus viennensis%, some attract strongly, some were less than the others. The non host plants were tested have neither attraction nor repulsion to the mite. The volatile from leaves of different host plants such as %Malus pumila, Pyrus bretschneideri, Amygdalus persica,Armeniaca vulgaris, Malus spectabili, Crataegus pinnatifida, Prunus yedoensis%, etc. Consists of trans-3-hexen-l-ol,acetate, trans-3-hexenyl ester butanolc acid, alpha farensene, etc., which do not exist in the non-host plants such as Euonymus japonicus.展开更多
【目的】明确室内条件下挥发性化合物苯并噻唑对韭菜迟眼蕈蚊Bradysia odoriphaga Yang et Zhang的生物活性。【方法】采用三角瓶密闭熏蒸法测定了苯并噻唑对韭菜迟眼蕈蚊成虫、卵、幼虫和蛹的熏蒸活性；利用Oxytherm氧电极研究了苯并...【目的】明确室内条件下挥发性化合物苯并噻唑对韭菜迟眼蕈蚊Bradysia odoriphaga Yang et Zhang的生物活性。【方法】采用三角瓶密闭熏蒸法测定了苯并噻唑对韭菜迟眼蕈蚊成虫、卵、幼虫和蛹的熏蒸活性；利用Oxytherm氧电极研究了苯并噻唑对韭菜迟眼蕈蚊成虫呼吸速率的影响；利用“Y”型嗅觉仪测定了韭菜迟眼蕈蚊成虫对苯并噻唑的行为反应。【结果】苯并噻唑对雌雄成虫处理0．5～2．0h的LC50分别为0．186～0．052和0．163～0．039μL／L；在0．01～0．13μL／L剂量下对卵熏蒸处理24h，第6天卵孵化率为4．83％一82．39％，而对照组孵化率为96．97％；苯并噻唑熏蒸4龄幼虫6～72h的LC50变化范围为1．247～0．248μL／L；0．01～0．09μL／L剂量熏蒸蛹24h，第5天的羽化率为8．17％～69．63％，对照组羽化率为96．23％。用0．052和0.039μL／L浓度分别处理雌雄成虫测定2．5h内呼吸速率的变化，表现为处理组初始呼吸速率明显高于对照组，然后逐渐降低至与对照组持平，最后明显低于相同处理时间对照组呼吸速率。“Y”型嗅觉仪测定结果表明，苯并噻唑对韭菜迟眼蕈蚊成虫有较强的引诱作用。在0．5L／min空气流速条件下，0．5止的苯并噻唑对雌雄成虫的引诱率分别为88．33％和78．53％。【结论】苯并噻唑对韭菜迟眼蕈蚊各虫态有很好的毒杀效果，并对成虫有强烈的引诱作用。展开更多
【目的】枣飞象Scythropus yasumatsui Kono et Morimoto是枣树重要害虫之一。近年来,在陕北黄河沿岸枣区暴发成灾,造成了严重的经济和生态损失。为了明确枣树挥发物在枣飞象寄主定向过程中的作用,以便为开发植物源引诱剂奠定理论基础...【目的】枣飞象Scythropus yasumatsui Kono et Morimoto是枣树重要害虫之一。近年来,在陕北黄河沿岸枣区暴发成灾,造成了严重的经济和生态损失。为了明确枣树挥发物在枣飞象寄主定向过程中的作用,以便为开发植物源引诱剂奠定理论基础。【方法】采用触角电位仪和Y型嗅觉仪测定了枣飞象对枣树7种挥发物的触角电位和嗅觉行为反应。【结果】EAG试验结果表明:枣树的7种挥发物在一定的浓度下,都能引起枣飞象雌雄成虫明显触角电位反应,当刺激浓度为50μg/μL时,枣飞象对测定的7种挥发物的触角电位值达到最高,其中枣飞象雌雄虫对罗勒烯的EAG反应相对值均为最大,分别达2.87和2.53。嗅觉行为反应试验结果表明:罗勒烯、α-法呢烯均对枣飞象雌雄虫有明显的引诱作用,其中枣飞象雌雄成虫对罗勒烯趋向率高达70.8%、70.4%,而反-2-己烯醇仅对其雌虫表现引诱作用,对雄虫未表现出引诱作用。【结论】罗勒烯和α-法呢烯为吸引枣飞象雌雄成虫的活性物质,本反-2-己烯醇为吸引枣飞象雌虫的活性物质。展开更多
文摘Olfactory respones of Tetranychus viennensis to different plants odors with “Y” olfactometer and petri-dish was studied and volatiles from leaves of different plants were absorbed with SPME and analyzed with GC-MS. It was showed: Olfaction takes part in the response during the Hawthorn Spider Mite looking for host plants, indicating %Tetranychus viennensis% were attracted by odors from host plants. Different host plants have different attraction to the %Tetranychus viennensis%, some attract strongly, some were less than the others. The non host plants were tested have neither attraction nor repulsion to the mite. The volatile from leaves of different host plants such as %Malus pumila, Pyrus bretschneideri, Amygdalus persica,Armeniaca vulgaris, Malus spectabili, Crataegus pinnatifida, Prunus yedoensis%, etc. Consists of trans-3-hexen-l-ol,acetate, trans-3-hexenyl ester butanolc acid, alpha farensene, etc., which do not exist in the non-host plants such as Euonymus japonicus.
文摘【目的】明确室内条件下挥发性化合物苯并噻唑对韭菜迟眼蕈蚊Bradysia odoriphaga Yang et Zhang的生物活性。【方法】采用三角瓶密闭熏蒸法测定了苯并噻唑对韭菜迟眼蕈蚊成虫、卵、幼虫和蛹的熏蒸活性；利用Oxytherm氧电极研究了苯并噻唑对韭菜迟眼蕈蚊成虫呼吸速率的影响；利用“Y”型嗅觉仪测定了韭菜迟眼蕈蚊成虫对苯并噻唑的行为反应。【结果】苯并噻唑对雌雄成虫处理0．5～2．0h的LC50分别为0．186～0．052和0．163～0．039μL／L；在0．01～0．13μL／L剂量下对卵熏蒸处理24h，第6天卵孵化率为4．83％一82．39％，而对照组孵化率为96．97％；苯并噻唑熏蒸4龄幼虫6～72h的LC50变化范围为1．247～0．248μL／L；0．01～0．09μL／L剂量熏蒸蛹24h，第5天的羽化率为8．17％～69．63％，对照组羽化率为96．23％。用0．052和0.039μL／L浓度分别处理雌雄成虫测定2．5h内呼吸速率的变化，表现为处理组初始呼吸速率明显高于对照组，然后逐渐降低至与对照组持平，最后明显低于相同处理时间对照组呼吸速率。“Y”型嗅觉仪测定结果表明，苯并噻唑对韭菜迟眼蕈蚊成虫有较强的引诱作用。在0．5L／min空气流速条件下，0．5止的苯并噻唑对雌雄成虫的引诱率分别为88．33％和78．53％。【结论】苯并噻唑对韭菜迟眼蕈蚊各虫态有很好的毒杀效果，并对成虫有强烈的引诱作用。
文摘【目的】枣飞象Scythropus yasumatsui Kono et Morimoto是枣树重要害虫之一。近年来,在陕北黄河沿岸枣区暴发成灾,造成了严重的经济和生态损失。为了明确枣树挥发物在枣飞象寄主定向过程中的作用,以便为开发植物源引诱剂奠定理论基础。【方法】采用触角电位仪和Y型嗅觉仪测定了枣飞象对枣树7种挥发物的触角电位和嗅觉行为反应。【结果】EAG试验结果表明:枣树的7种挥发物在一定的浓度下,都能引起枣飞象雌雄成虫明显触角电位反应,当刺激浓度为50μg/μL时,枣飞象对测定的7种挥发物的触角电位值达到最高,其中枣飞象雌雄虫对罗勒烯的EAG反应相对值均为最大,分别达2.87和2.53。嗅觉行为反应试验结果表明:罗勒烯、α-法呢烯均对枣飞象雌雄虫有明显的引诱作用,其中枣飞象雌雄成虫对罗勒烯趋向率高达70.8%、70.4%,而反-2-己烯醇仅对其雌虫表现引诱作用,对雄虫未表现出引诱作用。【结论】罗勒烯和α-法呢烯为吸引枣飞象雌雄成虫的活性物质,本反-2-己烯醇为吸引枣飞象雌虫的活性物质。
