Objective:To observe three species of Kalicephalus found in three species of snake(Ophkphagus hannah,Ptyas mucosus,and Naja Sputatrix) during research on Capture Snake for Trading in Java and Snake Biodiversity in Kal...Objective:To observe three species of Kalicephalus found in three species of snake(Ophkphagus hannah,Ptyas mucosus,and Naja Sputatrix) during research on Capture Snake for Trading in Java and Snake Biodiversity in Kalimantan Islands.Methods:Specimens for light microscopy examination were fixed with warm 70%alcohol,cleared and mounted in lactophenol for wet mounting.Drawings were made with the aid of a drawing tube attached to a Nikon compound microscope.Measurements were given in micrometers(μ) as the average of findings,followed by the range in parentheses,unless otherwise stated.Results:Kalkephalus(Costatus) indicus was found from 7 Ptyas mucosus,Kalicephalus bungari from 2 Naja sputatrix and 1 Kalicephalus(Costatus) indicus and Kalicephalus assimilis found from 1 Ophiophagus hannah.The morphology and measurement of three species of Kalicephalus found in this study were close to those described before.Conclusions:New finding of host of Kalicephalus(Costalus) indicus and Kalicephalus bungari was a snake species of Naja sputatrix.New records of locality were Kalimantan island as the new locality of Kalicephalus assimilis,and Java island was new locality of Kalicephalus(Costatus) indicus.展开更多
Cestode larvae spend one phase of their two-phase life cycle in the viscera of rodents, but cases of cestodes infecting subterranean rodents have only been rarely observed. To experimentally gain some insight into thi...Cestode larvae spend one phase of their two-phase life cycle in the viscera of rodents, but cases of cestodes infecting subterranean rodents have only been rarely observed. To experimentally gain some insight into this phenomenon, we captured approximately 300 plateau zokors(Eospalax baileyi), a typical subterranean rodent inhabiting the Qinghai-Tibet Plateau, and examined their livers for the presence of cysts. Totally, we collected five cysts, and using a mitochondrial gene(cox1) and two nuclear genes(pepck and pold) as genetic markers, we were able to analyze the taxonomy of the cysts. Both the maximum likelihood and Bayesian methods showed that the cysts share a monophyly with Taenia mustelae, while Kimura 2-parameter distances and number of different sites between our sequences and T. mustelae were far less than those found between the examined sequences and other Taeniidae species. These results, alongside supporting paraffin section histology, imply that the cysts found in plateau zokors can be regarded as larvae of T. mustelae, illustrating that zokors are a newly discovered intermediate host record of this parasite.展开更多
文摘Objective:To observe three species of Kalicephalus found in three species of snake(Ophkphagus hannah,Ptyas mucosus,and Naja Sputatrix) during research on Capture Snake for Trading in Java and Snake Biodiversity in Kalimantan Islands.Methods:Specimens for light microscopy examination were fixed with warm 70%alcohol,cleared and mounted in lactophenol for wet mounting.Drawings were made with the aid of a drawing tube attached to a Nikon compound microscope.Measurements were given in micrometers(μ) as the average of findings,followed by the range in parentheses,unless otherwise stated.Results:Kalkephalus(Costatus) indicus was found from 7 Ptyas mucosus,Kalicephalus bungari from 2 Naja sputatrix and 1 Kalicephalus(Costatus) indicus and Kalicephalus assimilis found from 1 Ophiophagus hannah.The morphology and measurement of three species of Kalicephalus found in this study were close to those described before.Conclusions:New finding of host of Kalicephalus(Costalus) indicus and Kalicephalus bungari was a snake species of Naja sputatrix.New records of locality were Kalimantan island as the new locality of Kalicephalus assimilis,and Java island was new locality of Kalicephalus(Costatus) indicus.
基金supported by the West Light Foundation of the Chinese Academy of Sciences and the Chinese Academy of Sciences President Scholarship(to G.Lin)
文摘Cestode larvae spend one phase of their two-phase life cycle in the viscera of rodents, but cases of cestodes infecting subterranean rodents have only been rarely observed. To experimentally gain some insight into this phenomenon, we captured approximately 300 plateau zokors(Eospalax baileyi), a typical subterranean rodent inhabiting the Qinghai-Tibet Plateau, and examined their livers for the presence of cysts. Totally, we collected five cysts, and using a mitochondrial gene(cox1) and two nuclear genes(pepck and pold) as genetic markers, we were able to analyze the taxonomy of the cysts. Both the maximum likelihood and Bayesian methods showed that the cysts share a monophyly with Taenia mustelae, while Kimura 2-parameter distances and number of different sites between our sequences and T. mustelae were far less than those found between the examined sequences and other Taeniidae species. These results, alongside supporting paraffin section histology, imply that the cysts found in plateau zokors can be regarded as larvae of T. mustelae, illustrating that zokors are a newly discovered intermediate host record of this parasite.
