目的检测神经元素3(neurogenin3,NEUROG3)在胃癌临床标本和细胞系中的表达,探讨其对胃癌细胞恶性表型的影响。方法采用免疫组织化学染色(immunohistochemistry,IHC)检测NEUROG3在肠化生胃黏膜和胃癌组织中的表达。利用Real-time PCR和We...目的检测神经元素3(neurogenin3,NEUROG3)在胃癌临床标本和细胞系中的表达,探讨其对胃癌细胞恶性表型的影响。方法采用免疫组织化学染色(immunohistochemistry,IHC)检测NEUROG3在肠化生胃黏膜和胃癌组织中的表达。利用Real-time PCR和Western blot检测NEUROG3在不同分化程度胃癌细胞系和人胃黏膜上皮细胞系GES-1中的表达。使用慢病毒转染沉默NEUROG3基因表达,Real-time PCR检测胃癌细胞分化抑制蛋白(inhibitor of DNA binding or differentiation,Id-1)的表达,利用EdU细胞增殖实验和细胞克隆形成实验检测胃癌细胞的增殖,划痕实验和Transwell实验检测胃癌细胞迁移和侵袭。结果NEUROG3在低分化胃癌组织中表达较低,而在癌旁组织、肠化生胃黏膜组织和中高分化胃癌组织中表达评分较高,评分差异有统计学意义(P<0.001)。NEUROG3的表达差异与患者性别、年龄和肿瘤大小无统计学意义(P>0.05),而与肿瘤分化程度(P<0.001,r=0.412)呈正相关,与淋巴结转移(P<0.05,r=-0.180)和TNM分期呈负相关(P<0.001,r=-0.431)。GES-1和高分化胃癌细胞系MKN-28中的NEUROG3表达明显高于中低分化胃癌细胞系SGC-7901、MKN-45和BGC-823(P<0.001)。MKN-28细胞沉默NEUROG3表达后,细胞分化程度降低,Id-1表达明显增高(P<0.001),细胞增殖、迁移和侵袭能力(P<0.01)明显增强。结论沉默NEUROG3基因表达,可抑制胃癌细胞的分化,促进胃癌细胞增殖、迁移和侵袭。展开更多
Inflammatory bowel disease(IBD) is a chronic recurrent condition whose etiology is unknown,and it includes ulcerative colitis,Crohn's disease,and microscopic colitis. These three diseases differ in clinical manife...Inflammatory bowel disease(IBD) is a chronic recurrent condition whose etiology is unknown,and it includes ulcerative colitis,Crohn's disease,and microscopic colitis. These three diseases differ in clinical manifestations,courses,and prognoses. IBD reduces the patients' quality of life and is an economic burden to both the patients and society. Interactions between the gastrointestinal(GI) neuroendocrine peptides/amines(NEPA) and the immune system are believed to play an important role in the pathophysiology of IBD. Moreover,the interaction between GI NEPA and intestinal microbiota appears to play also a pivotal role in the pathophysiology of IBD. This review summarizes the available data on GI NEPA in IBD,and speculates on their possible role in the pathophysiology and the potential use of this information when developing treatments. GI NEPA serotonin,the neuropeptide Y family,and substance P are proinflammatory,while the chromogranin/secretogranin family,vasoactive intestinal peptide,somatostatin,and ghrelin are antiinflammatory. Several innate and adaptive immune cells express these NEPA and/or have receptors to them. The GI NEPA are affected in patients with IBD and in animal models of human IBD. The GI NEPA are potentially useful for the diagnosis and follow-up of the activity of IBD,and are candidate targets for treatments of this disease.展开更多
文摘目的检测神经元素3(neurogenin3,NEUROG3)在胃癌临床标本和细胞系中的表达,探讨其对胃癌细胞恶性表型的影响。方法采用免疫组织化学染色(immunohistochemistry,IHC)检测NEUROG3在肠化生胃黏膜和胃癌组织中的表达。利用Real-time PCR和Western blot检测NEUROG3在不同分化程度胃癌细胞系和人胃黏膜上皮细胞系GES-1中的表达。使用慢病毒转染沉默NEUROG3基因表达,Real-time PCR检测胃癌细胞分化抑制蛋白(inhibitor of DNA binding or differentiation,Id-1)的表达,利用EdU细胞增殖实验和细胞克隆形成实验检测胃癌细胞的增殖,划痕实验和Transwell实验检测胃癌细胞迁移和侵袭。结果NEUROG3在低分化胃癌组织中表达较低,而在癌旁组织、肠化生胃黏膜组织和中高分化胃癌组织中表达评分较高,评分差异有统计学意义(P<0.001)。NEUROG3的表达差异与患者性别、年龄和肿瘤大小无统计学意义(P>0.05),而与肿瘤分化程度(P<0.001,r=0.412)呈正相关,与淋巴结转移(P<0.05,r=-0.180)和TNM分期呈负相关(P<0.001,r=-0.431)。GES-1和高分化胃癌细胞系MKN-28中的NEUROG3表达明显高于中低分化胃癌细胞系SGC-7901、MKN-45和BGC-823(P<0.001)。MKN-28细胞沉默NEUROG3表达后,细胞分化程度降低,Id-1表达明显增高(P<0.001),细胞增殖、迁移和侵袭能力(P<0.01)明显增强。结论沉默NEUROG3基因表达,可抑制胃癌细胞的分化,促进胃癌细胞增殖、迁移和侵袭。
文摘Inflammatory bowel disease(IBD) is a chronic recurrent condition whose etiology is unknown,and it includes ulcerative colitis,Crohn's disease,and microscopic colitis. These three diseases differ in clinical manifestations,courses,and prognoses. IBD reduces the patients' quality of life and is an economic burden to both the patients and society. Interactions between the gastrointestinal(GI) neuroendocrine peptides/amines(NEPA) and the immune system are believed to play an important role in the pathophysiology of IBD. Moreover,the interaction between GI NEPA and intestinal microbiota appears to play also a pivotal role in the pathophysiology of IBD. This review summarizes the available data on GI NEPA in IBD,and speculates on their possible role in the pathophysiology and the potential use of this information when developing treatments. GI NEPA serotonin,the neuropeptide Y family,and substance P are proinflammatory,while the chromogranin/secretogranin family,vasoactive intestinal peptide,somatostatin,and ghrelin are antiinflammatory. Several innate and adaptive immune cells express these NEPA and/or have receptors to them. The GI NEPA are affected in patients with IBD and in animal models of human IBD. The GI NEPA are potentially useful for the diagnosis and follow-up of the activity of IBD,and are candidate targets for treatments of this disease.
