基于网络药理学方法挖掘青盐方(Qingyan Formula,QYF)治疗围绝经期焦虑症(perimenopausal anxiety disorder,PAD)的潜能并进行药效学的实验验证。首先利用中药系统药理数据库和分析平台(Traditional Chinese Medicine Systems Pharmacol...基于网络药理学方法挖掘青盐方(Qingyan Formula,QYF)治疗围绝经期焦虑症(perimenopausal anxiety disorder,PAD)的潜能并进行药效学的实验验证。首先利用中药系统药理数据库和分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)检索青盐方中的药物化学成分,然后利用中医药整合药理学网络计算研究平台(Integrative Pharmacology-based Network Computational Research Platform of Traditional Chinese Medicine,TCMIP)v2.0和Swiss Target Prediction数据库筛选药物靶标,通过GeneCards和OMIM数据库查询PAD相关的靶标,筛选QYF和PAD的共有靶标,然后进行信号通路的富集分析;采用旷场试验和O迷宫试验验证QYF治疗PAD焦虑大鼠的药效学作用,并检测血清神经递质水平。结果显示,QYF可能发挥作用的类固醇激素合成、卵巢类固醇生成、雌激素信号通路等调控类固醇激素类信号通路,色氨酸代谢通路、Toll样受体信号通路、TGF-β信号通路等调控中枢神经递质,NF-κB信号通路、Apelin信号通路、MAPK信号通路、TNF信号通路、FOXO信号通路等调控炎症反应的信号通路均与PAD有关,说明QYF可能具有防治PAD的作用。青盐方70%乙醇提取物(Qingyan Formula 70%ethanol extract,QYFE)能延长去卵巢(ovariectomized,OVX)焦虑大鼠在旷场试验中央场的活动时间和活动距离,增加OVX焦虑大鼠在O迷宫试验中开臂区域的持续时间和活动距离,具有抗焦虑作用,且升高OVX焦虑大鼠血清5-羟色胺(5-hydroxytryptamine,5-HT)、γ氨基丁酸(gamma aminobutyric acid,GABA)水平,显著降低谷氨酸(glutamate,Glu)、去甲肾上腺素(norepinephrine,NA)水平,逆转OVX引起的兴奋性/抑制性递质平衡的紊乱,可能与其缓解OVX大鼠焦虑障碍有关。展开更多
This paper introduces Twist-routing, a new routing algorithm for faulty on-chip networks, which improves Maze-routing, a face-routing based algorithm which uses deflections in routing, and archives full fault coverage...This paper introduces Twist-routing, a new routing algorithm for faulty on-chip networks, which improves Maze-routing, a face-routing based algorithm which uses deflections in routing, and archives full fault coverage and fast packet delivery. To build Twist-routing algorithm, we use bounding circles, which borrows the idea from GOAFR+ routing algorithm for ad-hoc wireless networks. Unlike Maze-routing, whose path length is unbounded even when the optimal path length is fixed, in Twist-routing, the path length is bounded by the cube of the optimal path length. Our evaluations show that Twist-routing algorithm delivers packets up to 35% faster than Maze-routing with a uniform traffic and Erdos-Rényi failure model, when the failure rate and the injection rate vary.展开更多
基金Supported by the National Natural Science Foundation of China under Grant No.90412008(国家自然科学基金)the National Basic Research Program of China under Grant No.2004CB318204(国家重点基础研究发展计划(973))
文摘基于网络药理学方法挖掘青盐方(Qingyan Formula,QYF)治疗围绝经期焦虑症(perimenopausal anxiety disorder,PAD)的潜能并进行药效学的实验验证。首先利用中药系统药理数据库和分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)检索青盐方中的药物化学成分,然后利用中医药整合药理学网络计算研究平台(Integrative Pharmacology-based Network Computational Research Platform of Traditional Chinese Medicine,TCMIP)v2.0和Swiss Target Prediction数据库筛选药物靶标,通过GeneCards和OMIM数据库查询PAD相关的靶标,筛选QYF和PAD的共有靶标,然后进行信号通路的富集分析;采用旷场试验和O迷宫试验验证QYF治疗PAD焦虑大鼠的药效学作用,并检测血清神经递质水平。结果显示,QYF可能发挥作用的类固醇激素合成、卵巢类固醇生成、雌激素信号通路等调控类固醇激素类信号通路,色氨酸代谢通路、Toll样受体信号通路、TGF-β信号通路等调控中枢神经递质,NF-κB信号通路、Apelin信号通路、MAPK信号通路、TNF信号通路、FOXO信号通路等调控炎症反应的信号通路均与PAD有关,说明QYF可能具有防治PAD的作用。青盐方70%乙醇提取物(Qingyan Formula 70%ethanol extract,QYFE)能延长去卵巢(ovariectomized,OVX)焦虑大鼠在旷场试验中央场的活动时间和活动距离,增加OVX焦虑大鼠在O迷宫试验中开臂区域的持续时间和活动距离,具有抗焦虑作用,且升高OVX焦虑大鼠血清5-羟色胺(5-hydroxytryptamine,5-HT)、γ氨基丁酸(gamma aminobutyric acid,GABA)水平,显著降低谷氨酸(glutamate,Glu)、去甲肾上腺素(norepinephrine,NA)水平,逆转OVX引起的兴奋性/抑制性递质平衡的紊乱,可能与其缓解OVX大鼠焦虑障碍有关。
文摘This paper introduces Twist-routing, a new routing algorithm for faulty on-chip networks, which improves Maze-routing, a face-routing based algorithm which uses deflections in routing, and archives full fault coverage and fast packet delivery. To build Twist-routing algorithm, we use bounding circles, which borrows the idea from GOAFR+ routing algorithm for ad-hoc wireless networks. Unlike Maze-routing, whose path length is unbounded even when the optimal path length is fixed, in Twist-routing, the path length is bounded by the cube of the optimal path length. Our evaluations show that Twist-routing algorithm delivers packets up to 35% faster than Maze-routing with a uniform traffic and Erdos-Rényi failure model, when the failure rate and the injection rate vary.
