Background:This study aimed to investigate the effects of oral administration of Enterococcus faecium NCIMB10415(E.faecium)on intestinal development,immunological parameters and gut microbiota of neonatal piglets chal...Background:This study aimed to investigate the effects of oral administration of Enterococcus faecium NCIMB10415(E.faecium)on intestinal development,immunological parameters and gut microbiota of neonatal piglets challenged with enterotoxigenic Escherichia coli K88(ETEC).A total of 961-day-old sow-reared piglets were randomly assigned to 2 groups,with 48 piglets in each group.The piglets were from 16 litters(6 piglets each litter),and 3 piglets each litter were allocated to the E.faecium-supplemented(PRO)group,while the other 3 piglets were allocated to the control(CON)group.After colostrum intake,piglets in the PRO group were orally administrated with 3×10~9 CFU E.faecium per day for a period of one week.On day 8,one piglet per litter from each group was challenged(CON+ETEC,PRO+ETEC)or not(CON-ETEC,PRO-ETEC)with ETEC in a 2×2 factorial arrangement of treatments.On day 10(2 days after challenge),blood and tissue samples were obtained from piglets.Results:Before ETEC challenge,there were no significant differences for the average daily gain(ADG)and fecal score between the two groups of piglets.After ETEC challenge,the challenged piglets had greater fecal score compared to the non-challenged piglets,whereas E.faecium administration was able to decrease the fecal score.Piglets challenged with ETEC had shorter villous height,deeper crypt depth,and reduced number of goblet cells in the jejunum and decreased m RNA abundance of claudin-1 in the ileum,whereas increased the percentage of lymphocytes,concentrations of IL-1βin the plasma and TNF-αin the ileal mucosa,as well as increased the m RNA abundances of innate immunity-related genes in the ileum tissue.These deleterious effects caused by ETEC were partly alleviated by feeding E.faecium.In addition,piglets in PRO-ETEC group had decreased the percentage of CD8^+T cells of the peripheral blood when compared to those in CON-ETEC group.Moreover,E.faecium administration increased Verrucomicrobia at phylum level and decreased Bilophila at genus level.Conclusions:These r展开更多
包括2个饲养试验。实验一:取4对不同体重0日龄长太仔猪,窝内配对设计,3日龄分别补铁150 m g或不补铁。仔猪哺乳期间不补料,禁止仔猪接触母猪料、母猪粪。30日龄断奶,实验期47 d。实验二:取4对不同体重0日龄杜长太仔猪,设计、饲养同实验...包括2个饲养试验。实验一:取4对不同体重0日龄长太仔猪,窝内配对设计,3日龄分别补铁150 m g或不补铁。仔猪哺乳期间不补料,禁止仔猪接触母猪料、母猪粪。30日龄断奶,实验期47 d。实验二:取4对不同体重0日龄杜长太仔猪,设计、饲养同实验一,28 d结束实验。但实验二中的仔猪出生体重较大,平均达1.675 kg。结果表明:1)不补铁仔猪皮肤较苍白、被毛相对较粗乱及较安静,但腹泻频率差异不大;2)从生长性能看,补铁与否对出生时体重较轻的仔猪比较显著,但对出生时体重较重的仔猪影响不显著;3)从血液参数看,补铁与否的影响与相似于生长性能。结论:补铁对仔猪的影响可能与仔猪出生体重有关,出生体重大的仔猪可能并不一定需要补铁,或者可以采取更为温和的补铁方式。展开更多
将预产期、胎次相近的母猪20头随机分为试验组和对照组,每组2个重复,试验组仔猪饮用0.1%应激康5 m L,对照组饮用自来水5 m L,仔猪用母乳喂养。结果表明,试验组仔猪日增重较对照组提高17.92%,发病率降低23.94%,死亡率降低45.54%,Ig G水...将预产期、胎次相近的母猪20头随机分为试验组和对照组,每组2个重复,试验组仔猪饮用0.1%应激康5 m L,对照组饮用自来水5 m L,仔猪用母乳喂养。结果表明,试验组仔猪日增重较对照组提高17.92%,发病率降低23.94%,死亡率降低45.54%,Ig G水平提高8.30%。由此可知,微生态制剂具有促进仔猪生长、降低发病率和增强免疫力的作用。展开更多
基金supported by the Projects of The National Key Research and Development Program of China(grant number 2016YFD0501204)Sichuan provincial project on S&T application and demonstration(grant number2016CC0070)the project on commercialization of research findings under funding of government of Sichuan province(grant number16ZHSF0385).
文摘Background:This study aimed to investigate the effects of oral administration of Enterococcus faecium NCIMB10415(E.faecium)on intestinal development,immunological parameters and gut microbiota of neonatal piglets challenged with enterotoxigenic Escherichia coli K88(ETEC).A total of 961-day-old sow-reared piglets were randomly assigned to 2 groups,with 48 piglets in each group.The piglets were from 16 litters(6 piglets each litter),and 3 piglets each litter were allocated to the E.faecium-supplemented(PRO)group,while the other 3 piglets were allocated to the control(CON)group.After colostrum intake,piglets in the PRO group were orally administrated with 3×10~9 CFU E.faecium per day for a period of one week.On day 8,one piglet per litter from each group was challenged(CON+ETEC,PRO+ETEC)or not(CON-ETEC,PRO-ETEC)with ETEC in a 2×2 factorial arrangement of treatments.On day 10(2 days after challenge),blood and tissue samples were obtained from piglets.Results:Before ETEC challenge,there were no significant differences for the average daily gain(ADG)and fecal score between the two groups of piglets.After ETEC challenge,the challenged piglets had greater fecal score compared to the non-challenged piglets,whereas E.faecium administration was able to decrease the fecal score.Piglets challenged with ETEC had shorter villous height,deeper crypt depth,and reduced number of goblet cells in the jejunum and decreased m RNA abundance of claudin-1 in the ileum,whereas increased the percentage of lymphocytes,concentrations of IL-1βin the plasma and TNF-αin the ileal mucosa,as well as increased the m RNA abundances of innate immunity-related genes in the ileum tissue.These deleterious effects caused by ETEC were partly alleviated by feeding E.faecium.In addition,piglets in PRO-ETEC group had decreased the percentage of CD8^+T cells of the peripheral blood when compared to those in CON-ETEC group.Moreover,E.faecium administration increased Verrucomicrobia at phylum level and decreased Bilophila at genus level.Conclusions:These r
文摘将预产期、胎次相近的母猪20头随机分为试验组和对照组,每组2个重复,试验组仔猪饮用0.1%应激康5 m L,对照组饮用自来水5 m L,仔猪用母乳喂养。结果表明,试验组仔猪日增重较对照组提高17.92%,发病率降低23.94%,死亡率降低45.54%,Ig G水平提高8.30%。由此可知,微生态制剂具有促进仔猪生长、降低发病率和增强免疫力的作用。
