在跨语言的考察中,我们发现人类语言通常使用形态变化或结构位置表示否定,即通过否定副词、否定助词以及否定词缀来实现。手语是使用空间信息表达意义的语言符号,是一种与有声语言既有共性又有差异性的视觉语言符号系统。手语中的否定...在跨语言的考察中,我们发现人类语言通常使用形态变化或结构位置表示否定,即通过否定副词、否定助词以及否定词缀来实现。手语是使用空间信息表达意义的语言符号,是一种与有声语言既有共性又有差异性的视觉语言符号系统。手语中的否定标记既包含否定副词、否定助词、否定词缀,还包括非线性的否定标记,也称作非手挖否定标记(non-manual markers of negation),这类标记可以附着在词或句子之上,有时也可以单独承担否定的功能,它的位置和范围在不同的语言中会受到各自语言的特殊限制。文章以中国手语(CSL)为例,讨论手语中否定标记的类型、线性和非线性位置及辖域、分裂否定的表现形式,以及有声语言与视觉语言在类型学上的异同。展开更多
Objective: Neoadjuvant chemotherapy (NAC) was increasingly used as a systemic therapy for triple-negative breast cancer (TNBC). The pathological complete response (PCR) rates of neoadjuvant chemotherapy in TNBC were h...Objective: Neoadjuvant chemotherapy (NAC) was increasingly used as a systemic therapy for triple-negative breast cancer (TNBC). The pathological complete response (PCR) rates of neoadjuvant chemotherapy in TNBC were higher than other types of breast cancer with fluctuate data. Predictors to identify which subgroup TNBC was more likely to achieve PCR in neoadjuvant chemotherapy would give us some hints on how to improve outcomes of TNBC patients. The meta-analysis was conducted to contrast the prognostic function of some clinicopathological parameters in the PCR rates of neoadjuvant chemotherapy for TNBC. Methods: Studies were selected from the PubMed database. The relevant parameters to PCR rates in TNBC group were recorded. Review Manager and MIX were used to estimate prognostic function of some biological markers and clinicopathological parameters in PCR rates of TNBC. Results: The analysis included 6 studies with 723 patients, the aggregate PCR rate was 27.9% in TNBC group. The association of lymph nodes metastasis, Ki-67 expression, p53 expression and CK5/6 expression with PCR rate of TNBC was investigated in the analysis, and the odds ratios were 0.50, 9.87, 1.17 and 0.53 respectively. Conclusion: This meta-analysis demonstrated that Ki-67 expression and lymph nodes metastasis were predictors of PCR rate for TNBC in neoadjuvant chemotherapy, while p53 and CK5/6 expression could not be confirmed for the prognostic function.展开更多
The novel ET therapy is that doxorubicin and paclitaxel are used in neoadjuvant chemotherapy for tri-negative breast cancer(TNBC).The study mainly investigated the efficacy of ET therapy and its impact on tumor marker...The novel ET therapy is that doxorubicin and paclitaxel are used in neoadjuvant chemotherapy for tri-negative breast cancer(TNBC).The study mainly investigated the efficacy of ET therapy and its impact on tumor markers and cytokines.Firstly extracted from August 2017 to August 2020,84 cases of TNBC patients were as experimental group in our hospital,and the patients were randomly divided into two groups,including the control group(42 cases)underwent conventional chemotherapy and observation group(42 cases)to accept ET neoadjuvant chemotherapy.To compare the therapeutic effect of two groups,levels of cytokines,tumor markers,and survival were detected.The results showed that the total efficiency of the control group was 80.95%,significantly lower than that of the observation group(95.24%),and the difference between the two groups was statistically significant(P<0.05).The survival rate of the control group was 78.57%,which was significantly lower than that of the observation group(95.24%).In addition,CEA,CA19-9,CA125 and VEGF of the control group were significantly higher than those of the observation group(P<0.05).Therefore,the ET therapy of neoadjuvant chemotherapy has an ideal effect on the treatment of TNBC,which can reduce the levels of tumor markers and cytokines,prolonging the survival rate of patients.展开更多
Purpose:Triple-negative breast cancer(TNBC)is an aggressive subtype of breast cancer.It is still unclear that the mechanisms by which long non-coding RNA(lncRNA)regulates tumorigenesis of TNBC.In this study,we explore...Purpose:Triple-negative breast cancer(TNBC)is an aggressive subtype of breast cancer.It is still unclear that the mechanisms by which long non-coding RNA(lncRNA)regulates tumorigenesis of TNBC.In this study,we explored the function and regulation of lncRNA in TNBC.Methods:The diferentially expressed and overlapped lncRNAs were obtained from two microarray datasets of breast cancer.The cancer genome atlas(TCGA)data was applied to validate the roles of top diferentially expressed lncRNAs.The potential relationship among lncRNAs,miRNAs,and mRNAs and the efects of them on the TNBC tumorigenesis were further explored through multiple bioinformatic methods.Results:Long intergenic non-protein coding RNA 1351(LINC01351)was frst discovered to play an oncogenic role in TNBC.The highly expressed LINC01351 was signifcantly related to aggressive subtypes,advanced stages and metastasis of breast cancer.The overexpressed LINC01351 was associated with adverse prognosis of patients with TNBC.LINC01351 was found to negatively regulate ELK4 which was involved in the transcriptional regulation in cancer.The high expression of ELK4 showed favorable prognosis of patients with basal-like 1 subtype and luminal androgen receptor subtype of TNBC.Conclusion:The dysregulation of LINC01351 played an important role in triple-negative breast cancer.LINC01351 could be a poor prognostic marker and a potential target for patients with TNBC.展开更多
为了研制口蹄疫抗原表位突变标记疫苗,本研究以含有Asia 1型口蹄疫病毒(FMDV)c DNA全长的感染性克隆p Asia 1-FMDV作为骨架,将3D蛋白中第27位氨基酸的H和31位的氨基酸N分别突变成Y和R,从而突变3D蛋白的一个抗原表位,将构建的带有突变表...为了研制口蹄疫抗原表位突变标记疫苗,本研究以含有Asia 1型口蹄疫病毒(FMDV)c DNA全长的感染性克隆p Asia 1-FMDV作为骨架,将3D蛋白中第27位氨基酸的H和31位的氨基酸N分别突变成Y和R,从而突变3D蛋白的一个抗原表位,将构建的带有突变表位的重组质粒转染BHK-21细胞,成功拯救出一株突变FMDV。经比较后发现,重组病毒的生物学特性与亲本毒株相似。病毒中和试验结果显示,抗重组病毒的血清与亲本病毒有良好的反应性。Western blotting结果表明重组病毒诱导的抗体能与突变的表位合成肽反应而不与野生型病毒的表位合成肽发生反应,从而区分重组病毒与亲本病毒。综上所述,这株抗原表位突变FMDV有望作为口蹄疫标记疫苗候株进一步评估。展开更多
文摘在跨语言的考察中,我们发现人类语言通常使用形态变化或结构位置表示否定,即通过否定副词、否定助词以及否定词缀来实现。手语是使用空间信息表达意义的语言符号,是一种与有声语言既有共性又有差异性的视觉语言符号系统。手语中的否定标记既包含否定副词、否定助词、否定词缀,还包括非线性的否定标记,也称作非手挖否定标记(non-manual markers of negation),这类标记可以附着在词或句子之上,有时也可以单独承担否定的功能,它的位置和范围在不同的语言中会受到各自语言的特殊限制。文章以中国手语(CSL)为例,讨论手语中否定标记的类型、线性和非线性位置及辖域、分裂否定的表现形式,以及有声语言与视觉语言在类型学上的异同。
基金Supported by a grant from the Science&Technology Supporting Foundation of Liaoning Province(No.20102060)
文摘Objective: Neoadjuvant chemotherapy (NAC) was increasingly used as a systemic therapy for triple-negative breast cancer (TNBC). The pathological complete response (PCR) rates of neoadjuvant chemotherapy in TNBC were higher than other types of breast cancer with fluctuate data. Predictors to identify which subgroup TNBC was more likely to achieve PCR in neoadjuvant chemotherapy would give us some hints on how to improve outcomes of TNBC patients. The meta-analysis was conducted to contrast the prognostic function of some clinicopathological parameters in the PCR rates of neoadjuvant chemotherapy for TNBC. Methods: Studies were selected from the PubMed database. The relevant parameters to PCR rates in TNBC group were recorded. Review Manager and MIX were used to estimate prognostic function of some biological markers and clinicopathological parameters in PCR rates of TNBC. Results: The analysis included 6 studies with 723 patients, the aggregate PCR rate was 27.9% in TNBC group. The association of lymph nodes metastasis, Ki-67 expression, p53 expression and CK5/6 expression with PCR rate of TNBC was investigated in the analysis, and the odds ratios were 0.50, 9.87, 1.17 and 0.53 respectively. Conclusion: This meta-analysis demonstrated that Ki-67 expression and lymph nodes metastasis were predictors of PCR rate for TNBC in neoadjuvant chemotherapy, while p53 and CK5/6 expression could not be confirmed for the prognostic function.
文摘The novel ET therapy is that doxorubicin and paclitaxel are used in neoadjuvant chemotherapy for tri-negative breast cancer(TNBC).The study mainly investigated the efficacy of ET therapy and its impact on tumor markers and cytokines.Firstly extracted from August 2017 to August 2020,84 cases of TNBC patients were as experimental group in our hospital,and the patients were randomly divided into two groups,including the control group(42 cases)underwent conventional chemotherapy and observation group(42 cases)to accept ET neoadjuvant chemotherapy.To compare the therapeutic effect of two groups,levels of cytokines,tumor markers,and survival were detected.The results showed that the total efficiency of the control group was 80.95%,significantly lower than that of the observation group(95.24%),and the difference between the two groups was statistically significant(P<0.05).The survival rate of the control group was 78.57%,which was significantly lower than that of the observation group(95.24%).In addition,CEA,CA19-9,CA125 and VEGF of the control group were significantly higher than those of the observation group(P<0.05).Therefore,the ET therapy of neoadjuvant chemotherapy has an ideal effect on the treatment of TNBC,which can reduce the levels of tumor markers and cytokines,prolonging the survival rate of patients.
基金supported by the following grants:Foundation of Young Innovative Talents (Tianjin Medical University Cancer Institute and Hospital,2018-1-31)National Science Foundation of China (NSFC No.81401950)+2 种基金China Scholarship Council (File No.201706940019)Tianjin Key Medical Discipline (Specialty)Construction ProjectTianjin Medical University Cancer Hospital"14th Five-Year"Peak Discipline Support Program Project.
文摘Purpose:Triple-negative breast cancer(TNBC)is an aggressive subtype of breast cancer.It is still unclear that the mechanisms by which long non-coding RNA(lncRNA)regulates tumorigenesis of TNBC.In this study,we explored the function and regulation of lncRNA in TNBC.Methods:The diferentially expressed and overlapped lncRNAs were obtained from two microarray datasets of breast cancer.The cancer genome atlas(TCGA)data was applied to validate the roles of top diferentially expressed lncRNAs.The potential relationship among lncRNAs,miRNAs,and mRNAs and the efects of them on the TNBC tumorigenesis were further explored through multiple bioinformatic methods.Results:Long intergenic non-protein coding RNA 1351(LINC01351)was frst discovered to play an oncogenic role in TNBC.The highly expressed LINC01351 was signifcantly related to aggressive subtypes,advanced stages and metastasis of breast cancer.The overexpressed LINC01351 was associated with adverse prognosis of patients with TNBC.LINC01351 was found to negatively regulate ELK4 which was involved in the transcriptional regulation in cancer.The high expression of ELK4 showed favorable prognosis of patients with basal-like 1 subtype and luminal androgen receptor subtype of TNBC.Conclusion:The dysregulation of LINC01351 played an important role in triple-negative breast cancer.LINC01351 could be a poor prognostic marker and a potential target for patients with TNBC.
