目的测定宣痹洗剂中羟基红花黄色素A的含量。方法采用高效液相色谱法,以羟基红花黄色素A含量为指标,通过正交试验确定正丁醇萃取的最佳条件并测定其含量。结果宣痹洗剂中羟基红花黄色素A的最佳正丁醇萃取条件为正丁醇用量60 m L,萃取4次...目的测定宣痹洗剂中羟基红花黄色素A的含量。方法采用高效液相色谱法,以羟基红花黄色素A含量为指标,通过正交试验确定正丁醇萃取的最佳条件并测定其含量。结果宣痹洗剂中羟基红花黄色素A的最佳正丁醇萃取条件为正丁醇用量60 m L,萃取4次,每次振摇50 s,各因素影响顺序为正丁醇用量>萃取次数>振摇时间,测得宣痹洗剂中羟基红花黄色素A的含量为0.39μg/m L,平均回收率为97.06%,RSD=1.4%(n=6)。结论本研究建立的正丁醇萃取方法与含量测定方法简便快速、精密度高,可用于该制剂的质量控制。展开更多
The n-butanol extract from Parthenocissus tricuspidata leaves were examined for antimicrobial activity against five microbes by using filter paper method.The results showed that the extract restrained the growth of Es...The n-butanol extract from Parthenocissus tricuspidata leaves were examined for antimicrobial activity against five microbes by using filter paper method.The results showed that the extract restrained the growth of Escherichia coli,Agrobacterium tumefaciens,Bacillus subitilis and Bacillus pumilus,while inhibited the growth of yeast at a certain extent.And the minimum inhibitory concentrations(MIC) were 0.25,0.3,0.25,0.3,1.0 g·mL-1respectively.Moreover the n-butanol extract also could restrained some bacterium strains,which had antibiotic resistances.After heated at 95 ℃ for 2～3 h,the n-butanol extract from P.tricuspidata leaves still presented strong bacteriostasis to germs.This result indicated that the substance had heat-stability.展开更多
文摘目的测定宣痹洗剂中羟基红花黄色素A的含量。方法采用高效液相色谱法,以羟基红花黄色素A含量为指标,通过正交试验确定正丁醇萃取的最佳条件并测定其含量。结果宣痹洗剂中羟基红花黄色素A的最佳正丁醇萃取条件为正丁醇用量60 m L,萃取4次,每次振摇50 s,各因素影响顺序为正丁醇用量>萃取次数>振摇时间,测得宣痹洗剂中羟基红花黄色素A的含量为0.39μg/m L,平均回收率为97.06%,RSD=1.4%(n=6)。结论本研究建立的正丁醇萃取方法与含量测定方法简便快速、精密度高,可用于该制剂的质量控制。
文摘The n-butanol extract from Parthenocissus tricuspidata leaves were examined for antimicrobial activity against five microbes by using filter paper method.The results showed that the extract restrained the growth of Escherichia coli,Agrobacterium tumefaciens,Bacillus subitilis and Bacillus pumilus,while inhibited the growth of yeast at a certain extent.And the minimum inhibitory concentrations(MIC) were 0.25,0.3,0.25,0.3,1.0 g·mL-1respectively.Moreover the n-butanol extract also could restrained some bacterium strains,which had antibiotic resistances.After heated at 95 ℃ for 2～3 h,the n-butanol extract from P.tricuspidata leaves still presented strong bacteriostasis to germs.This result indicated that the substance had heat-stability.
