Objective:To investigate the protective effect and mechanism of lithium chloride pretreatment on myocardial ischemia-reperfusion injury(I-RI)in rats.Methods:A total of 60 SD rats were randomly divided into control gro...Objective:To investigate the protective effect and mechanism of lithium chloride pretreatment on myocardial ischemia-reperfusion injury(I-RI)in rats.Methods:A total of 60 SD rats were randomly divided into control group,model group,lithium chloride intervention group and L-arginine methyl ester+lithium chloride intervention group with 15 in each.The I-RI model was established in model group,the Utliium chloride intervention group and L-arginine methyl ester+lithium chloride intervention group by method of seaming along left anterior descending coronary artery myocardial,control group was only opened the chest without seaming,STelevation within 2 min was regarded as modeling success.Model group did not adopted any intervention,lithium chloride intervention group was treated with lithium chloride injection15 mg/kg by jugular venipuncture preoperatively,L-arginine methyl ester+lithium chloride intervention group was treated with intraperitoneal injection of 30mg·kg～(-1)·d～(-1)(L—arginine methyl ester 7 d before the test,and intravenous catheter of 15 mg/kg lithium chloride preoperatively.The hydroxybulyric acid dehydrogenase(HBDH),creatine kinase isoenzyme(CK-MB),superoxide dismutase(SOD),malondialdehyde(MDA)level and nitric oxide synthase(NOS)activites were tested.Each large area of myocardial ischemia tissue was extracted for determination of the MDA content,SOD activity in tissue and serum,and morphological changes of myocardial tissue.Results:SOD activity was highest in lithium chloride intervention group,followed by L-arginine methyl ester+lithium chloride intervention group,control group and model group(P<0.05);SOD activity was highest in L-arginine methyl ester+lithium chloride intervention group intervention group,followed by lithium chloride intervention group,control group and model group(P<0.05).MDA content of myocardial tissue was highest in model group,followed by L-arginine methyl ester+lithium chloride intervention group,the lithium chloride intervention group and control group(P<0.05);serum M展开更多
基金supported by Target Scientific Research of Zhangjiakou Technology Bureau No.12110044D-2
文摘Objective:To investigate the protective effect and mechanism of lithium chloride pretreatment on myocardial ischemia-reperfusion injury(I-RI)in rats.Methods:A total of 60 SD rats were randomly divided into control group,model group,lithium chloride intervention group and L-arginine methyl ester+lithium chloride intervention group with 15 in each.The I-RI model was established in model group,the Utliium chloride intervention group and L-arginine methyl ester+lithium chloride intervention group by method of seaming along left anterior descending coronary artery myocardial,control group was only opened the chest without seaming,STelevation within 2 min was regarded as modeling success.Model group did not adopted any intervention,lithium chloride intervention group was treated with lithium chloride injection15 mg/kg by jugular venipuncture preoperatively,L-arginine methyl ester+lithium chloride intervention group was treated with intraperitoneal injection of 30mg·kg～(-1)·d～(-1)(L—arginine methyl ester 7 d before the test,and intravenous catheter of 15 mg/kg lithium chloride preoperatively.The hydroxybulyric acid dehydrogenase(HBDH),creatine kinase isoenzyme(CK-MB),superoxide dismutase(SOD),malondialdehyde(MDA)level and nitric oxide synthase(NOS)activites were tested.Each large area of myocardial ischemia tissue was extracted for determination of the MDA content,SOD activity in tissue and serum,and morphological changes of myocardial tissue.Results:SOD activity was highest in lithium chloride intervention group,followed by L-arginine methyl ester+lithium chloride intervention group,control group and model group(P<0.05);SOD activity was highest in L-arginine methyl ester+lithium chloride intervention group intervention group,followed by lithium chloride intervention group,control group and model group(P<0.05).MDA content of myocardial tissue was highest in model group,followed by L-arginine methyl ester+lithium chloride intervention group,the lithium chloride intervention group and control group(P<0.05);serum M
