Sequencing data from 10 species show that a plant hexokinase （HXK） family contains 5-11 genes. Functionally, a given family can include metabolic catalysts, glucose signaling proteins, and non-catalytic, apparent re...Sequencing data from 10 species show that a plant hexokinase （HXK） family contains 5-11 genes. Functionally, a given family can include metabolic catalysts, glucose signaling proteins, and non-catalytic, apparent regulatory enzyme homologs. This study has two goals. The first aim is to develop a predictive method to determine which HXK proteins within a species have which type of function. The second aim is to determine whether HXK-dependent glucose signaling proteins occur among more primitive plants, as well as among angiosperms. Using a molecular phylogeny ap- proach, combined with selective experimental testing, we found that non-catalytic HXK homologs might occur in all plants, including the relatively primitive Selaginella moellendorffi. We also found that different lineages of angiosperm HXKs have apparent conserved features for catalytic activity and for sub-cellular targeting. Most higher-plant HXKs are expressed predominantly at mitochondria, with HXKs of one lineage occurring in the plastid, and HXKs of one monocot lineage occurring in the cytosol. Using protoplast transient expression assays, we found that HXK glucose signaling pro- teins occur likely in all higher plants and in S. moellendorffi as well. Thus, the use of glucose by plant HXK isoforms in metabolism and/or as a regulatory metabolite occurs as widespread, conserved processes.展开更多
目的筛选与自身免疫病相关兼职蛋白的B细胞优势表位肽并进行血清学检定。方法利用DNAMAN软件分析不同物种热休克蛋白70(heat shock protein 70, HSP70)和α-烯醇酶氨基酸序列同源性,IEDB数据库和瓜氨酸化在线平台预测线性B细胞表位和瓜...目的筛选与自身免疫病相关兼职蛋白的B细胞优势表位肽并进行血清学检定。方法利用DNAMAN软件分析不同物种热休克蛋白70(heat shock protein 70, HSP70)和α-烯醇酶氨基酸序列同源性,IEDB数据库和瓜氨酸化在线平台预测线性B细胞表位和瓜氨酸化修饰位点;间接ELISA检测抗HSP70和α-烯醇酶蛋白瓜氨酸化前后表位肽抗体。结果同源性分析和IEDB数据库预测得到兼职蛋白HSP70和α-烯醇酶各10个B细胞优势表位肽;瓜氨酸化位点预测显示,其分别有9个和4个位点可能会发生瓜氨酸化修饰,其中5个瓜氨酸化修饰位点与预测表位肽重合。与正常人相比,抗环瓜氨酸肽抗体阳性患者血清抗瓜氨酸化修饰表位肽的抗体滴度明显增高(P<0.05)。结论采用生物信息学分析和ELISA初步验证的方法有助于筛选自身抗原相关的B细胞优势表位肽。展开更多
文摘Sequencing data from 10 species show that a plant hexokinase （HXK） family contains 5-11 genes. Functionally, a given family can include metabolic catalysts, glucose signaling proteins, and non-catalytic, apparent regulatory enzyme homologs. This study has two goals. The first aim is to develop a predictive method to determine which HXK proteins within a species have which type of function. The second aim is to determine whether HXK-dependent glucose signaling proteins occur among more primitive plants, as well as among angiosperms. Using a molecular phylogeny ap- proach, combined with selective experimental testing, we found that non-catalytic HXK homologs might occur in all plants, including the relatively primitive Selaginella moellendorffi. We also found that different lineages of angiosperm HXKs have apparent conserved features for catalytic activity and for sub-cellular targeting. Most higher-plant HXKs are expressed predominantly at mitochondria, with HXKs of one lineage occurring in the plastid, and HXKs of one monocot lineage occurring in the cytosol. Using protoplast transient expression assays, we found that HXK glucose signaling pro- teins occur likely in all higher plants and in S. moellendorffi as well. Thus, the use of glucose by plant HXK isoforms in metabolism and/or as a regulatory metabolite occurs as widespread, conserved processes.
文摘目的筛选与自身免疫病相关兼职蛋白的B细胞优势表位肽并进行血清学检定。方法利用DNAMAN软件分析不同物种热休克蛋白70(heat shock protein 70, HSP70)和α-烯醇酶氨基酸序列同源性,IEDB数据库和瓜氨酸化在线平台预测线性B细胞表位和瓜氨酸化修饰位点;间接ELISA检测抗HSP70和α-烯醇酶蛋白瓜氨酸化前后表位肽抗体。结果同源性分析和IEDB数据库预测得到兼职蛋白HSP70和α-烯醇酶各10个B细胞优势表位肽;瓜氨酸化位点预测显示,其分别有9个和4个位点可能会发生瓜氨酸化修饰,其中5个瓜氨酸化修饰位点与预测表位肽重合。与正常人相比,抗环瓜氨酸肽抗体阳性患者血清抗瓜氨酸化修饰表位肽的抗体滴度明显增高(P<0.05)。结论采用生物信息学分析和ELISA初步验证的方法有助于筛选自身抗原相关的B细胞优势表位肽。
