摘 要: 利用RAPD标记以银耳属的2个种为外类群研究了木耳属8个种的分子系统发育关系。根据UPGMA 构建的树状图结果表明木耳属(Auricularia)和银耳属(Tremella)分别为单一的分类单元。木耳属的8个种被分成3个明显不同的组,第一组包括盾...摘 要: 利用RAPD标记以银耳属的2个种为外类群研究了木耳属8个种的分子系统发育关系。根据UPGMA 构建的树状图结果表明木耳属(Auricularia)和银耳属(Tremella)分别为单一的分类单元。木耳属的8个种被分成3个明显不同的组,第一组包括盾形木耳(A.peltata Lloyd)和角质木耳(A.cornea Spreng.),第二组包括所有供试的黑木耳(A.auricula Underw.),第三组包括皱木耳(A.delicata Henn.)、琥珀木耳(A.fuscosuccinea Farl.)、毛木耳(A.polytricha Sacc.)、大木耳(A.maxima Y. R. Guo)和网膜木耳(A.reticulata L. J. Li)五个形态种。种间发育关系表明角质木耳和毛木耳应是两个不同的种而非同种异名,子实体横切面中髓层的有无并不表明不同种间亲缘关系的远近。展开更多
为了探讨DNA条形码技术在夜蛾物种鉴定中的可行性,本研究利用条形码通用引物扩增了北京百花山地区43种夜蛾75个样本的线粒体细胞色素C氧化酶亚基I(mitochondrial cytochrome c oxidase subunitI,COI)基因序列,以Kimura双参数模型进行种...为了探讨DNA条形码技术在夜蛾物种鉴定中的可行性,本研究利用条形码通用引物扩增了北京百花山地区43种夜蛾75个样本的线粒体细胞色素C氧化酶亚基I(mitochondrial cytochrome c oxidase subunitI,COI)基因序列,以Kimura双参数模型进行种内种间遗传距离分析、使用邻接法(neighbor-joining,NJ)和最大简约法(maximumparsimony,MP)分别构建系统发育树,并利用分子序列差异阈值对样本进行分子可操作分类单元(molecular defined operational taxonomic units,MOTU)划分。结果表明:所有夜蛾种类通过系统发育树可以成功区分;种内平均遗传距离(0.03%)远远小于种间平均遗传距离(11.29%);采用较为保守的1%的序列差异阈值将75个夜蛾样本分为42个MOTU,正确率为95%,除了MOTU04包含2个物种外,剩余41个MOTU与形态种呈现一一对应的关系。结果显示,基于COI基因的DNA条形码对于本研究中所涉及的夜蛾具有较好的区分,可以作为一种有效的工具在夜蛾科昆虫物种鉴定中进行应用。展开更多
Clematis is a botanical source for various pharmaceutically active components,which has long been used in conventional medicine since the beginning of Chinese civilization.Increasing interest in Clematis medicinal res...Clematis is a botanical source for various pharmaceutically active components,which has long been used in conventional medicine since the beginning of Chinese civilization.Increasing interest in Clematis medicinal resources has led to additional discoveries of triterpenoid saponins,flavonoids,coumarins,alkaloids and many other compounds in various Clematis species,and to investigations on their chemotaxonomy,molecular phylogeny and pharmacology.In continuation with our studies on Clematis chemistry and biology,we review the chemistry,chemotaxonomy,molecular biology and phylogeny of Clematis and their relevance to drug efficacy and drug development.Various databases and technology have been used in literature search in order to characterize the global scientific effort.It is essential to study more species for both the sustainable utilization of Clematis medicinal resources and finding novel compounds with potential clinical utility.Systems biology and omics technologies will play an increasingly important role in future medicinal research involving bioactive compounds of Clematis.展开更多
文摘摘 要: 利用RAPD标记以银耳属的2个种为外类群研究了木耳属8个种的分子系统发育关系。根据UPGMA 构建的树状图结果表明木耳属(Auricularia)和银耳属(Tremella)分别为单一的分类单元。木耳属的8个种被分成3个明显不同的组,第一组包括盾形木耳(A.peltata Lloyd)和角质木耳(A.cornea Spreng.),第二组包括所有供试的黑木耳(A.auricula Underw.),第三组包括皱木耳(A.delicata Henn.)、琥珀木耳(A.fuscosuccinea Farl.)、毛木耳(A.polytricha Sacc.)、大木耳(A.maxima Y. R. Guo)和网膜木耳(A.reticulata L. J. Li)五个形态种。种间发育关系表明角质木耳和毛木耳应是两个不同的种而非同种异名,子实体横切面中髓层的有无并不表明不同种间亲缘关系的远近。
文摘为了探讨DNA条形码技术在夜蛾物种鉴定中的可行性,本研究利用条形码通用引物扩增了北京百花山地区43种夜蛾75个样本的线粒体细胞色素C氧化酶亚基I(mitochondrial cytochrome c oxidase subunitI,COI)基因序列,以Kimura双参数模型进行种内种间遗传距离分析、使用邻接法(neighbor-joining,NJ)和最大简约法(maximumparsimony,MP)分别构建系统发育树,并利用分子序列差异阈值对样本进行分子可操作分类单元(molecular defined operational taxonomic units,MOTU)划分。结果表明:所有夜蛾种类通过系统发育树可以成功区分;种内平均遗传距离(0.03%)远远小于种间平均遗传距离(11.29%);采用较为保守的1%的序列差异阈值将75个夜蛾样本分为42个MOTU,正确率为95%,除了MOTU04包含2个物种外,剩余41个MOTU与形态种呈现一一对应的关系。结果显示,基于COI基因的DNA条形码对于本研究中所涉及的夜蛾具有较好的区分,可以作为一种有效的工具在夜蛾科昆虫物种鉴定中进行应用。
基金supported by Dalian Jiaotong University and Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine,Ministry of Education,China
文摘Clematis is a botanical source for various pharmaceutically active components,which has long been used in conventional medicine since the beginning of Chinese civilization.Increasing interest in Clematis medicinal resources has led to additional discoveries of triterpenoid saponins,flavonoids,coumarins,alkaloids and many other compounds in various Clematis species,and to investigations on their chemotaxonomy,molecular phylogeny and pharmacology.In continuation with our studies on Clematis chemistry and biology,we review the chemistry,chemotaxonomy,molecular biology and phylogeny of Clematis and their relevance to drug efficacy and drug development.Various databases and technology have been used in literature search in order to characterize the global scientific effort.It is essential to study more species for both the sustainable utilization of Clematis medicinal resources and finding novel compounds with potential clinical utility.Systems biology and omics technologies will play an increasingly important role in future medicinal research involving bioactive compounds of Clematis.
