Benefiting from the development of molecular electronics and molecular plasmonics, the interplay of light and electronic transport in molecular junctions has attracted growing interest among researchers in both fields...Benefiting from the development of molecular electronics and molecular plasmonics, the interplay of light and electronic transport in molecular junctions has attracted growing interest among researchers in both fields, leading to a new research direction of "single-molecule optoelectronics". Here, we review the latest developments of photo-modulated charge transport,electroluminescence and Raman spectroscopy from single-molecule junctions, and suggest future directions for single-molecule optoelectronics.展开更多
In this article, we report on the characterization of various molecular junctions＇ current-voltage characteristics （Ⅰ-Ⅴ curves） evolution under mechanical modulations, by employing a novel electrochemically assis...In this article, we report on the characterization of various molecular junctions＇ current-voltage characteristics （Ⅰ-Ⅴ curves） evolution under mechanical modulations, by employing a novel electrochemically assisted-mechanically controllable break junction （EC-MCBJ） method. For 1,4-benzenedithiol, the Ⅰ-Ⅴ curves measured at constant electrode pair separation show excellent reproducibility, indicating the feasibility of our EC-MCBJ method for fabricating molecular junctions. For ferrocene-bisvinylphenylmethyl dithiol （Fc-VPM）, an anomalous type of Ⅰ-Ⅴ curve was observed by the particular control over the stepping motor. This phenomenon is rationalized assuming a model of atomic contact evolution with the presence of molecular junctions. To test this hypothesized model, a molecule with a longer length, 1,3-butadiyne-linked dinuclear ruthenium（H） complex （Ru-1）, was implemented, and the Ⅰ-Ⅴ curve evolution was investigated under similar circumstances. Compared with Fc-VPM, the observed Ⅰ-Ⅴ curves show close analogy and minor differences, and both of them fit the hypothesized model well.展开更多
Charge transport characterization of single-molecule junctions is essential for the fundamental research of single-molecule physical chemistry and the development towards single-molecule electronic devices and circuit...Charge transport characterization of single-molecule junctions is essential for the fundamental research of single-molecule physical chemistry and the development towards single-molecule electronic devices and circuits. Among the single-molecule conductance characterization techniques,the single-molecule break junction technique is widely used in tens of worldwide research laboratories which can generate a large amount of experimental data from thousands of individual measurement cycles. However,data interpretation is a challenging task for researchers with different research backgrounds,and the different data analysis approaches sometimes lead to the misunderstanding of the measurement data and even reproducibility issues of the measurement. It is thus a necessity to develop a user-friendly all-in-one data analysis tool that automatizes the basic data analysis in a standard and widely accepted way. In this work,we present the XMe Code (Xiamen Molecular Electronics Code),an intelligent all-in-one data analysis tool for the comprehensive analysis of single-molecule break junction data. XMe code provides end-to-end data analysis that takes in the original experimental data and returns electronic characteristics and even charge transport mechanisms. We believe that XMe Code will promote the transparency of the data analysis in single-molecule electronics and the collaborations among scientists with different research backgrounds.展开更多
Quantum tunneling conductance of molecular junctions originates from the charge transport through theπ-orbitals(π-transport)and theσ-orbitals(σ-transport)of the molecules,but theσ-transport can not be observed du...Quantum tunneling conductance of molecular junctions originates from the charge transport through theπ-orbitals(π-transport)and theσ-orbitals(σ-transport)of the molecules,but theσ-transport can not be observed due to the more rapid decay of the tunneling conductance in theσ-system compared to that in theπ-system.Here,we demonstrate that dominantσ-transport can be observed inπ-conjugated molecular junctions at the sub-nanometer scale using the scanning tunneling microscope break junction technique(STM-BJ).We have found that the conductance of meta-connected picolinic acid,which mainly occurs byσ-transport,is∼35 times higher than that of its para-isomer,which is entirely different from what is expected fromπ-transport through these systems.Flicker noise analysis reveals that the transport through the meta-connection exhibits more through-bond transport than the para-counterpart and density functional theory(DFT)shows that theσ-system provides the dominant transport path.These results reveal that theσ-electrons,rather than theπ-electrons,can dominate charge transport through conjugated molecular junctions at the sub-nanometer scale,and this provides a new avenue toward the future miniaturization of molecular devices and materials.展开更多
Utilizing density functional theory(DFT)and non-equilibrium Green's function,we systematically studied the electrical transport and rectification properties of thiol-and amino-terminated molecules embedded in grap...Utilizing density functional theory(DFT)and non-equilibrium Green's function,we systematically studied the electrical transport and rectification properties of thiol-and amino-terminated molecules embedded in graphene nanoribbons.We firstly found the thiol-terminated moleculesshowbetterelectron transport properties compared to the amino-terminated,which can be attributed to the strong electronwithdrawing ability and favorable coupling effects.Secondly,the symmetrical molecules show almost symmetrical current-voltage(-V)curves and exhibit negligible rectification effects.On the other hand,the asymmetrical molecules exhibit asymmetrical I-V curves and better rectification performance.The rectification effect is closely related to molecular asymmetry degrees.For example,the rectification ratio of asymmetric N6((E)-N1-(3-aminopropyl)-but-2-ene-1,4-diamine)molecule is much smaller than the N4(5-phenylthiazole-2,4-diamine)and N5(2,6-diaminohexane-1,1,5-triol)molecules.Furthermore,we found the rectification ratio of the asymmetrical amino-terminated molecules can reach 400,while the biggest rectification ratio of the thiol-terminated molecule can only reach 45.These findings offer crucial insights for future graphene molecular electronic device design.展开更多
Plasmonic optical manipulation has emerged as an affordable alternative to manipulate single chemical and biological molecules in nanoscience.Although the theoretical models of sub-5 nm single-molecule trapping have b...Plasmonic optical manipulation has emerged as an affordable alternative to manipulate single chemical and biological molecules in nanoscience.Although the theoretical models of sub-5 nm single-molecule trapping have been considered promising,the experimental strategies remain a challenge due to the Brownian motions and weak optical gradient forces with significantly reduced molecular polarizability.Herein,we address direct trapping and in situ sensing of single molecules with unprecedented size,down to∼5Åin solution,by employing an adjustable plasmonic optical nanogap and single-molecule conductance measurement.The theoretical simulations demonstrate that local fields with a high enhancement factor,over 103,were generated at such small nanogaps,resulting in optical forces as large as several piconewtons to suppress the Brownian motion and trap a molecule of length sub-1 nm.This work demonstrates a strategy for directly manipulating the small molecule units,promising a vast multitude of applications in chemical,biological,and materials sciences at the single-molecule level.展开更多
We have combined molecular dynamics simulations with first-principles calculations to study electron transport in a single molecular junction of perylene tetracarboxylic diimide (PTCDI) in aqueous solution under exter...We have combined molecular dynamics simulations with first-principles calculations to study electron transport in a single molecular junction of perylene tetracarboxylic diimide (PTCDI) in aqueous solution under external electric gate fields. It is found that the statistics of the molecular conductance are very sensitive to the strength of the electric field. The statistics of the molecular conductance are strongly associated with the thermal fluctuation of the water molecules around the PTCDI molecule. Our simulations reproduce the experimentally observed three orders of magnitude enhancement of the conductance, as well as the temperature dependent conductance, under the electrochemical gates. The effects of the molecular polarization and the dipole rearrangement of the aqueous solution are also discussed.展开更多
Recent years have witnessed the fabrication of various non-covalent interaction-based molecular electronic devices.In the noncovalent interaction-based molecular devices,the strength of the interfacial coupling betwee...Recent years have witnessed the fabrication of various non-covalent interaction-based molecular electronic devices.In the noncovalent interaction-based molecular devices,the strength of the interfacial coupling between molecule and electrode is weakened compared to that of the covalent interaction-based molecular devices,which provides wide applications in fabricating versatile molecular devices.In this review,we start with the methods capable of fabricating graphene-based nanogaps,and the following routes to construct non-covalent interaction-based molecular junctions with graphene electrodes.Then we give an introduction to the reported non-covalent interaction-based molecular devices with graphene electrodes equipped with different electrical functions.Moreover,we summarize the recent progress in the design and fabrication of new-type molecular devices based on graphene and graphene-like two-dimensional(2D)materials.The review ends with a prospect on the challenges and opportunities of non-covalent interaction-based molecular electronics in the near future.展开更多
Single-molecule devices,which are fabricated by the single molecule bridged through electrodes,provide a promising approach to investigate the intrinsic chemical or physical properties of individual molecules.Beyond t...Single-molecule devices,which are fabricated by the single molecule bridged through electrodes,provide a promising approach to investigate the intrinsic chemical or physical properties of individual molecules.Beyond the studies of single-molecule wires,a large number of responsive single-molecule junctions or devices with unique chemical or physical properties have been designed and fabricated by introducing the external field,which further offers the chance to explore conductive materials at the molecular level.Here,we summarized the latest studies on the behaviors of single-molecule devices based on the photon,thermal,electric,or magnetic responses,and discussed the development of responsive single-molecule devices in prospect.展开更多
A study on the fabrication and characterization of gold nanowires containing a 4,4′-azopyridine monolayer junctions was reported. Au nanowires were prepared by using a two-step alternative current(AC) electrodepositi...A study on the fabrication and characterization of gold nanowires containing a 4,4′-azopyridine monolayer junctions was reported. Au nanowires were prepared by using a two-step alternative current(AC) electrodeposition inside the pores of AAO. Azopyridine molecules were adsorbed on the top of Au layer by self-assembly and electroless plating was done to introduce Au caps on the top of the SAMs. The top Au segments were prepared by using AC electrodeposition, too. The HRTEM image shows the thickness of the SAM is approximately 1.5 nm, which is in good agreement with the theoretical length of azopyridine. It is demonstrated that direct AC electrodeposition was more effective than chemical deposition on strengthening the electronic device. Utilizing 4,4′-azopyridine as the active component, current-voltage characterization of nanowires containing molecules was done at room temperature by a two-point probe method by using a Keithley 4200 semiconductor characterization system. A good conductive behavior at room temperature in this electronic device was observed.展开更多
A first-principles computational method based on the hybrid density functional theory is developed to simulate the electronic transport properties of oligomeric phenylene ethynylene molecular junctions with H2O molecu...A first-principles computational method based on the hybrid density functional theory is developed to simulate the electronic transport properties of oligomeric phenylene ethynylene molecular junctions with H2O molecules accumulated in the vicinity as recently reported by Na et al. [Nanotechnology 18 424001 （2007）]. The numerical results show that the hydrogen bonds between the oxygen atoms of the oligomeric phenylene ethynylene molecule and H2O molecules result in the localisation of the molecular orbitals and lead to the lower transition peaks. The H2O molecular chains accumulated in the vicinity of the molecular junction can not only change the electronic structure of the molecular junctions, but also open additional electronic transport pathways. The obvious influence of H2O molecules on the electronic structure of the molecular junction and its electronic transport properties is thus demonstrated.展开更多
We present a novel ab initio non-equilibrium approach to calculate the current across a molecular junction. The method rests on a wavefunction-based full ab initio description of the central region of the junction com...We present a novel ab initio non-equilibrium approach to calculate the current across a molecular junction. The method rests on a wavefunction-based full ab initio description of the central region of the junction combined with a tight binding approximation for the electrodes in the frame of the Keldysh Green function formalism. Our procedure is demonstrated for a dithiolethine molecule located between silver electrodes. The main conducting channel is identified and the full current voltage characteristic is calculated.展开更多
Cadherin receptors mediate cell-cell adhesion, signal transduction and assembly of cytoskeletons. How a single transmembrane molecule Cadherin can be involved in multiple functions through modulating its binding activ...Cadherin receptors mediate cell-cell adhesion, signal transduction and assembly of cytoskeletons. How a single transmembrane molecule Cadherin can be involved in multiple functions through modulating its binding activities with many membrane adhesion molecules and cytoskeletal components is an unanswered question which can be elucidated by clues from bead experiments. Human lung cells expressing N-Cadherin were examined. After co-incubation with anti-N-Cadherin monoclonal antibody coated beads, cell surface clustering of N-Cadherin was induced. Immunofluorescent detection demonstrated that in addition to Cadherin, β-Catenin, α-Catenin, a-Actinin and Actin fluorescence also aggregated respectively at the membrane site of bead attachment. Myosin heavy chain (MHC), another major component of Actin cytoskeleton, did not aggregate at the membrane site of bead attachment. Adhesion unrelated protein Con A and polylysine conjugated beads did not induce the clustering of adhesion molecules. It is indicated展开更多
基金supported by the National Key R&D Program of China (2017YFA0204901, 2017YFA0204902)the National Natural Science Foundation of China (21673195, 61571242, 21503179, 21727806, 21722305)the Young Thousand Talent Project of China
文摘Benefiting from the development of molecular electronics and molecular plasmonics, the interplay of light and electronic transport in molecular junctions has attracted growing interest among researchers in both fields, leading to a new research direction of "single-molecule optoelectronics". Here, we review the latest developments of photo-modulated charge transport,electroluminescence and Raman spectroscopy from single-molecule junctions, and suggest future directions for single-molecule optoelectronics.
基金This work was supported by the National Basic Research Program of China （Nos. 2011YQ030124, 2014CB845603, and 2015CB932301）, National Natural Science Foundation of China （Nos. 91427304, 21321062, 21303114, 21403181, and 21503179）, Natural Science Foundation of Fujian Province （No. 2012J05034）, and by CNRS UMR 8640 PASTEUR and LIA CNRS NanoBioCatEchem.
文摘In this article, we report on the characterization of various molecular junctions＇ current-voltage characteristics （Ⅰ-Ⅴ curves） evolution under mechanical modulations, by employing a novel electrochemically assisted-mechanically controllable break junction （EC-MCBJ） method. For 1,4-benzenedithiol, the Ⅰ-Ⅴ curves measured at constant electrode pair separation show excellent reproducibility, indicating the feasibility of our EC-MCBJ method for fabricating molecular junctions. For ferrocene-bisvinylphenylmethyl dithiol （Fc-VPM）, an anomalous type of Ⅰ-Ⅴ curve was observed by the particular control over the stepping motor. This phenomenon is rationalized assuming a model of atomic contact evolution with the presence of molecular junctions. To test this hypothesized model, a molecule with a longer length, 1,3-butadiyne-linked dinuclear ruthenium（H） complex （Ru-1）, was implemented, and the Ⅰ-Ⅴ curve evolution was investigated under similar circumstances. Compared with Fc-VPM, the observed Ⅰ-Ⅴ curves show close analogy and minor differences, and both of them fit the hypothesized model well.
基金supported by the National Natural Science Foundation of China(22325303,21973079,22032004)the National Key R&D Program of China(2017YFA0204902)+2 种基金the Fundamental Research Funds for the Central Universities in China(Xiamen University,20720190002)IRTSTFJ,National Science Foundation of Fujian Province(2018J06004)Beijing National Laboratory for Molecular Sciences(BNLMS202005).
文摘Charge transport characterization of single-molecule junctions is essential for the fundamental research of single-molecule physical chemistry and the development towards single-molecule electronic devices and circuits. Among the single-molecule conductance characterization techniques,the single-molecule break junction technique is widely used in tens of worldwide research laboratories which can generate a large amount of experimental data from thousands of individual measurement cycles. However,data interpretation is a challenging task for researchers with different research backgrounds,and the different data analysis approaches sometimes lead to the misunderstanding of the measurement data and even reproducibility issues of the measurement. It is thus a necessity to develop a user-friendly all-in-one data analysis tool that automatizes the basic data analysis in a standard and widely accepted way. In this work,we present the XMe Code (Xiamen Molecular Electronics Code),an intelligent all-in-one data analysis tool for the comprehensive analysis of single-molecule break junction data. XMe code provides end-to-end data analysis that takes in the original experimental data and returns electronic characteristics and even charge transport mechanisms. We believe that XMe Code will promote the transparency of the data analysis in single-molecule electronics and the collaborations among scientists with different research backgrounds.
基金supported by the National Natural Science Foundation of China(21722305,21673195,21973079,and 21703188)the National Key R&D Program of China(2017YFA0204902)the Guangdong Basic and Applied Basic Research Foundation(2020A151511106).
文摘Quantum tunneling conductance of molecular junctions originates from the charge transport through theπ-orbitals(π-transport)and theσ-orbitals(σ-transport)of the molecules,but theσ-transport can not be observed due to the more rapid decay of the tunneling conductance in theσ-system compared to that in theπ-system.Here,we demonstrate that dominantσ-transport can be observed inπ-conjugated molecular junctions at the sub-nanometer scale using the scanning tunneling microscope break junction technique(STM-BJ).We have found that the conductance of meta-connected picolinic acid,which mainly occurs byσ-transport,is∼35 times higher than that of its para-isomer,which is entirely different from what is expected fromπ-transport through these systems.Flicker noise analysis reveals that the transport through the meta-connection exhibits more through-bond transport than the para-counterpart and density functional theory(DFT)shows that theσ-system provides the dominant transport path.These results reveal that theσ-electrons,rather than theπ-electrons,can dominate charge transport through conjugated molecular junctions at the sub-nanometer scale,and this provides a new avenue toward the future miniaturization of molecular devices and materials.
基金This work was supported by the National Natural Science Foundation of China(No.22073053)the Young Taishan Scholar Program of Shandong Province(No.tsqn201909139)+3 种基金the Program for Scientific Research Innovation Team in Colleges and Universities of Jinan(No.2021GXRC042)the Program for Introduced Innovation Teams from the New Collegiate 20 Items of Jinan(No.202228031)the Natural Science Foundation of Shandong Province(No.ZR2023MA089)Qilu University of Technology(Shandong Academy of Sciences)Basic Research Project of Science,Education and Industry Integration Pilot(No.2023PY046).
文摘Utilizing density functional theory(DFT)and non-equilibrium Green's function,we systematically studied the electrical transport and rectification properties of thiol-and amino-terminated molecules embedded in graphene nanoribbons.We firstly found the thiol-terminated moleculesshowbetterelectron transport properties compared to the amino-terminated,which can be attributed to the strong electronwithdrawing ability and favorable coupling effects.Secondly,the symmetrical molecules show almost symmetrical current-voltage(-V)curves and exhibit negligible rectification effects.On the other hand,the asymmetrical molecules exhibit asymmetrical I-V curves and better rectification performance.The rectification effect is closely related to molecular asymmetry degrees.For example,the rectification ratio of asymmetric N6((E)-N1-(3-aminopropyl)-but-2-ene-1,4-diamine)molecule is much smaller than the N4(5-phenylthiazole-2,4-diamine)and N5(2,6-diaminohexane-1,1,5-triol)molecules.Furthermore,we found the rectification ratio of the asymmetrical amino-terminated molecules can reach 400,while the biggest rectification ratio of the thiol-terminated molecule can only reach 45.These findings offer crucial insights for future graphene molecular electronic device design.
基金supported by the National Natural Science Foundation of China(grant nos.T2222002,21973079,22032004,92161118,12174324,21991130,and 21905238)the Ministry of Science and Technology of the People’s Republic of China(grant no.2021YFA1201502)the Natural Science Foundation of Fujian Province(grant no.2021J06008).
文摘Plasmonic optical manipulation has emerged as an affordable alternative to manipulate single chemical and biological molecules in nanoscience.Although the theoretical models of sub-5 nm single-molecule trapping have been considered promising,the experimental strategies remain a challenge due to the Brownian motions and weak optical gradient forces with significantly reduced molecular polarizability.Herein,we address direct trapping and in situ sensing of single molecules with unprecedented size,down to∼5Åin solution,by employing an adjustable plasmonic optical nanogap and single-molecule conductance measurement.The theoretical simulations demonstrate that local fields with a high enhancement factor,over 103,were generated at such small nanogaps,resulting in optical forces as large as several piconewtons to suppress the Brownian motion and trap a molecule of length sub-1 nm.This work demonstrates a strategy for directly manipulating the small molecule units,promising a vast multitude of applications in chemical,biological,and materials sciences at the single-molecule level.
基金This work was supported by the Swedish Research Council(VR),the Swedish National Infrastructure for Computing(SNIC)the Natural Science Foundation of China(No.20825312)and the Fok Ying Tong Education Foundation(No.111013).
文摘We have combined molecular dynamics simulations with first-principles calculations to study electron transport in a single molecular junction of perylene tetracarboxylic diimide (PTCDI) in aqueous solution under external electric gate fields. It is found that the statistics of the molecular conductance are very sensitive to the strength of the electric field. The statistics of the molecular conductance are strongly associated with the thermal fluctuation of the water molecules around the PTCDI molecule. Our simulations reproduce the experimentally observed three orders of magnitude enhancement of the conductance, as well as the temperature dependent conductance, under the electrochemical gates. The effects of the molecular polarization and the dipole rearrangement of the aqueous solution are also discussed.
基金the support from the National Natural Science Foundation of China(Nos.21973079 and 22032004)the National Key R&D Program of China(No.2017YFA0204902)the Fundamental Research Funds for the Central Universities(Xiamen University:No.20720190002).
文摘Recent years have witnessed the fabrication of various non-covalent interaction-based molecular electronic devices.In the noncovalent interaction-based molecular devices,the strength of the interfacial coupling between molecule and electrode is weakened compared to that of the covalent interaction-based molecular devices,which provides wide applications in fabricating versatile molecular devices.In this review,we start with the methods capable of fabricating graphene-based nanogaps,and the following routes to construct non-covalent interaction-based molecular junctions with graphene electrodes.Then we give an introduction to the reported non-covalent interaction-based molecular devices with graphene electrodes equipped with different electrical functions.Moreover,we summarize the recent progress in the design and fabrication of new-type molecular devices based on graphene and graphene-like two-dimensional(2D)materials.The review ends with a prospect on the challenges and opportunities of non-covalent interaction-based molecular electronics in the near future.
基金This work was supported by the National Natural Science Foundation of China(Nos.21722305,21673195)the National Key R&D Program of China(2017YFA0204902)the Fundamental Research Funds for the Central Universities in China(Xiamen University)(20720190002).
文摘Single-molecule devices,which are fabricated by the single molecule bridged through electrodes,provide a promising approach to investigate the intrinsic chemical or physical properties of individual molecules.Beyond the studies of single-molecule wires,a large number of responsive single-molecule junctions or devices with unique chemical or physical properties have been designed and fabricated by introducing the external field,which further offers the chance to explore conductive materials at the molecular level.Here,we summarized the latest studies on the behaviors of single-molecule devices based on the photon,thermal,electric,or magnetic responses,and discussed the development of responsive single-molecule devices in prospect.
文摘A study on the fabrication and characterization of gold nanowires containing a 4,4′-azopyridine monolayer junctions was reported. Au nanowires were prepared by using a two-step alternative current(AC) electrodeposition inside the pores of AAO. Azopyridine molecules were adsorbed on the top of Au layer by self-assembly and electroless plating was done to introduce Au caps on the top of the SAMs. The top Au segments were prepared by using AC electrodeposition, too. The HRTEM image shows the thickness of the SAM is approximately 1.5 nm, which is in good agreement with the theoretical length of azopyridine. It is demonstrated that direct AC electrodeposition was more effective than chemical deposition on strengthening the electronic device. Utilizing 4,4′-azopyridine as the active component, current-voltage characterization of nanowires containing molecules was done at room temperature by a two-point probe method by using a Keithley 4200 semiconductor characterization system. A good conductive behavior at room temperature in this electronic device was observed.
基金Project supported by the National Natural Science Foundation of China (Grant Nos.10804064 and 10674084)
文摘A first-principles computational method based on the hybrid density functional theory is developed to simulate the electronic transport properties of oligomeric phenylene ethynylene molecular junctions with H2O molecules accumulated in the vicinity as recently reported by Na et al. [Nanotechnology 18 424001 （2007）]. The numerical results show that the hydrogen bonds between the oxygen atoms of the oligomeric phenylene ethynylene molecule and H2O molecules result in the localisation of the molecular orbitals and lead to the lower transition peaks. The H2O molecular chains accumulated in the vicinity of the molecular junction can not only change the electronic structure of the molecular junctions, but also open additional electronic transport pathways. The obvious influence of H2O molecules on the electronic structure of the molecular junction and its electronic transport properties is thus demonstrated.
文摘We present a novel ab initio non-equilibrium approach to calculate the current across a molecular junction. The method rests on a wavefunction-based full ab initio description of the central region of the junction combined with a tight binding approximation for the electrodes in the frame of the Keldysh Green function formalism. Our procedure is demonstrated for a dithiolethine molecule located between silver electrodes. The main conducting channel is identified and the full current voltage characteristic is calculated.
文摘Cadherin receptors mediate cell-cell adhesion, signal transduction and assembly of cytoskeletons. How a single transmembrane molecule Cadherin can be involved in multiple functions through modulating its binding activities with many membrane adhesion molecules and cytoskeletal components is an unanswered question which can be elucidated by clues from bead experiments. Human lung cells expressing N-Cadherin were examined. After co-incubation with anti-N-Cadherin monoclonal antibody coated beads, cell surface clustering of N-Cadherin was induced. Immunofluorescent detection demonstrated that in addition to Cadherin, β-Catenin, α-Catenin, a-Actinin and Actin fluorescence also aggregated respectively at the membrane site of bead attachment. Myosin heavy chain (MHC), another major component of Actin cytoskeleton, did not aggregate at the membrane site of bead attachment. Adhesion unrelated protein Con A and polylysine conjugated beads did not induce the clustering of adhesion molecules. It is indicated
