An acclimatized mixed microbial culture,predominantly Pseudomonas sp.,was enriched from a sewage treatment plant,and its potential to simultaneously degrade mixtures of phenol and m-cresol was investigated during its ...An acclimatized mixed microbial culture,predominantly Pseudomonas sp.,was enriched from a sewage treatment plant,and its potential to simultaneously degrade mixtures of phenol and m-cresol was investigated during its growth in batch shake flasks.A 22 full factorial design with the two substrates at two different levels and different initial concentration ranges(low and high),was employed to carry out the biodegradation experiments.The substrates phenol and m-cresol were completely utilized within 21 h when ...展开更多
Four sequence batch reactors（SBRs） fed by fermented sugar cane wastewater were continuously operated under the aerobic dynamic feeding（ADF） mode with different configurations of sludge retention time（SRT）, carbo...Four sequence batch reactors（SBRs） fed by fermented sugar cane wastewater were continuously operated under the aerobic dynamic feeding（ADF） mode with different configurations of sludge retention time（SRT）, carbon and initial biomass concentrations to enrich polyhydroxyalkanoate（PHA） accumulating mixed microbial cultures（MMCs） from municipal activated sludge.The stability of SBRs was investigated besides the enrichment performance. The microbial community structures of the enriched MMCs were analyzed using terminal restriction fragment length polymorphism（T-RFLP）. The optimum operating conditions for the enrichment process were： SRT of 5 days, carbon concentration of 2.52 g COD/L and initial biomass concentration of3.65 g/L. The best enrichment performance in terms of both operating stability and PHA storage ability of enriched cultures（with the maximum PHA content and PHA storage yield（YPHA/S） of61.26% and 0.68 mg COD/mg COD, respectively） was achieved under this condition. Effects of the SRT, carbon concentration and initial biomass concentration on the PHA accumulating MMCs selection process were discussed respectively. A new model including the segmentation of the enrichment process and the effects of SRT on each phase was proposed.展开更多
文摘An acclimatized mixed microbial culture,predominantly Pseudomonas sp.,was enriched from a sewage treatment plant,and its potential to simultaneously degrade mixtures of phenol and m-cresol was investigated during its growth in batch shake flasks.A 22 full factorial design with the two substrates at two different levels and different initial concentration ranges(low and high),was employed to carry out the biodegradation experiments.The substrates phenol and m-cresol were completely utilized within 21 h when ...
基金supported by the National Natural Science Foundation of China(No.51378142)the Program for New Century Excellent Talents in University(No.NCET-12-0156)+1 种基金the Open Project of the State Key Laboratory of Urban Water ResourceEnvironment(Harbin institute of Technology)(No.2015DX10)
文摘Four sequence batch reactors（SBRs） fed by fermented sugar cane wastewater were continuously operated under the aerobic dynamic feeding（ADF） mode with different configurations of sludge retention time（SRT）, carbon and initial biomass concentrations to enrich polyhydroxyalkanoate（PHA） accumulating mixed microbial cultures（MMCs） from municipal activated sludge.The stability of SBRs was investigated besides the enrichment performance. The microbial community structures of the enriched MMCs were analyzed using terminal restriction fragment length polymorphism（T-RFLP）. The optimum operating conditions for the enrichment process were： SRT of 5 days, carbon concentration of 2.52 g COD/L and initial biomass concentration of3.65 g/L. The best enrichment performance in terms of both operating stability and PHA storage ability of enriched cultures（with the maximum PHA content and PHA storage yield（YPHA/S） of61.26% and 0.68 mg COD/mg COD, respectively） was achieved under this condition. Effects of the SRT, carbon concentration and initial biomass concentration on the PHA accumulating MMCs selection process were discussed respectively. A new model including the segmentation of the enrichment process and the effects of SRT on each phase was proposed.
