AIM: To understand the pathogen characteristics and its sensitivity against antimicrobial agents in fatal bacterial granuloma after eyelid trauma (FBGT) in vitro, and to provide laboratory evidence for diagnosis. METH...AIM: To understand the pathogen characteristics and its sensitivity against antimicrobial agents in fatal bacterial granuloma after eyelid trauma (FBGT) in vitro, and to provide laboratory evidence for diagnosis. METHODS: The FBGT pathogens were isolaated and cultured with reformed rabbit-brain anaerobic enriched broth (RRAB), and identified by ATB/API 20A system. The minimum inhibiting concentration (MIC) was determined by anaerobic broth dilution method. RESULTS: A total of 22 strains of pathogen were separated from 21 patients with FBGT and identified as Propionibacterium acnes (PA) by ATB/API 20A system. The MIC of ciprofloxacin for 22 PA strains was 0.0625-0.5mg/L, the MIC of penicillin, ampicillin, ampicillin/sulbactam, cefoperazone, lincomycin, and imipenem/cilastatin were 0.125-0.5mg/L, the MIC of ticarcillin/clavulanic acid was 0.250-1.000mg/L, and the MIC of metronidazole was 64-256mg/L. The pathogen of FBGT was strictly anaerobic PA, which growed slowly and better in nutritious RRAB broth. All PA were resistant to metronidazole, but susceptive to other routine antimicrobial agents, such as penicillin, ampicillin and lincomycin. CONCLUSION: FBGT should not be treated with metronidazole. Clinicians should choose combined use of drugs or operation to treat FBGT according to patients' individual condition and the results of drug sensitivity test.展开更多
根据美国国家标准委员会制定的真菌体外药敏试验方法M-27A,M-38P,测定了那他霉素对黄曲霉菌、青霉菌、毛霉菌和白念珠菌的最小抑菌浓度,对那他霉素的抑菌和杀菌特性进行研究,结果表明:那他霉素对青霉菌的最小抑菌浓度为2.75 m g/kg,对...根据美国国家标准委员会制定的真菌体外药敏试验方法M-27A,M-38P,测定了那他霉素对黄曲霉菌、青霉菌、毛霉菌和白念珠菌的最小抑菌浓度,对那他霉素的抑菌和杀菌特性进行研究,结果表明:那他霉素对青霉菌的最小抑菌浓度为2.75 m g/kg,对黄曲霉菌为5.5 m g/kg,对毛霉菌为5.5 m g/kg,对白念珠菌为2.75 m g/kg.展开更多
文摘AIM: To understand the pathogen characteristics and its sensitivity against antimicrobial agents in fatal bacterial granuloma after eyelid trauma (FBGT) in vitro, and to provide laboratory evidence for diagnosis. METHODS: The FBGT pathogens were isolaated and cultured with reformed rabbit-brain anaerobic enriched broth (RRAB), and identified by ATB/API 20A system. The minimum inhibiting concentration (MIC) was determined by anaerobic broth dilution method. RESULTS: A total of 22 strains of pathogen were separated from 21 patients with FBGT and identified as Propionibacterium acnes (PA) by ATB/API 20A system. The MIC of ciprofloxacin for 22 PA strains was 0.0625-0.5mg/L, the MIC of penicillin, ampicillin, ampicillin/sulbactam, cefoperazone, lincomycin, and imipenem/cilastatin were 0.125-0.5mg/L, the MIC of ticarcillin/clavulanic acid was 0.250-1.000mg/L, and the MIC of metronidazole was 64-256mg/L. The pathogen of FBGT was strictly anaerobic PA, which growed slowly and better in nutritious RRAB broth. All PA were resistant to metronidazole, but susceptive to other routine antimicrobial agents, such as penicillin, ampicillin and lincomycin. CONCLUSION: FBGT should not be treated with metronidazole. Clinicians should choose combined use of drugs or operation to treat FBGT according to patients' individual condition and the results of drug sensitivity test.
文摘根据美国国家标准委员会制定的真菌体外药敏试验方法M-27A,M-38P,测定了那他霉素对黄曲霉菌、青霉菌、毛霉菌和白念珠菌的最小抑菌浓度,对那他霉素的抑菌和杀菌特性进行研究,结果表明:那他霉素对青霉菌的最小抑菌浓度为2.75 m g/kg,对黄曲霉菌为5.5 m g/kg,对毛霉菌为5.5 m g/kg,对白念珠菌为2.75 m g/kg.
