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马尾松种子园的遗传多样性与父本分析 被引量:41
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作者 艾畅 徐立安 +2 位作者 赖焕林 黄敏仁 王章荣 《林业科学》 EI CAS CSCD 北大核心 2006年第11期146-150,共5页
Eleven microsatellite(SSR)markers were used to evaluate the genetic diversity and paternal parents of a seed orchard for Pinus massoniana planted at Wuyi Forest Farm,Fujian Province.The seed orchard contains 286 open_... Eleven microsatellite(SSR)markers were used to evaluate the genetic diversity and paternal parents of a seed orchard for Pinus massoniana planted at Wuyi Forest Farm,Fujian Province.The seed orchard contains 286 open_pollinated progenies derived from 129 candidate paternal clones and three maternal trees.Both the parental and progeny populations displayed high genetic diversity(h),with the estimated h values of 0.543 8 and 0.455 6,respectively.Paternal clones contributed differently to the progeny;only 5.3%~15.8% clones contributed 27.08%~48.96% pollen.More strikingly,one paternal clone contributed 10% and 17% pollen,respectively,to two maternal trees.It was found that the mating compatibility of a clone was not related to its origin.There was no significant influence of the physical location of a clone on the amount of paternal constituent and genetic diversity.The rate of contamination by pollen outside the seed orchard was as high as 9.44%.No selfing progeny was observed,suggesting that selfing depression will not be an issue in the seed orchard of P.massoniana. 展开更多
关键词 马尾松 种子园 遗传多样性 父本分析 微卫星(ssr)
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油松种子园自由授粉与控制授粉种子父本分析 被引量:12
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作者 张冬梅 孙佩光 +1 位作者 沈熙环 茹广欣 《植物生态学报》 CAS CSCD 北大核心 2009年第2期302-310,共9页
利用"微卫星"标记对油松(Pinus tabulaeformis)种子园控制授粉和自由授粉的子代进行了父本分析,探讨自由授粉状态下种子园子代遗传组成与花粉传播的关系,以及人工控制授粉状态下花粉形态等指标与子代遗传组成的相关性。其中,... 利用"微卫星"标记对油松(Pinus tabulaeformis)种子园控制授粉和自由授粉的子代进行了父本分析,探讨自由授粉状态下种子园子代遗传组成与花粉传播的关系,以及人工控制授粉状态下花粉形态等指标与子代遗传组成的相关性。其中,利用SSR对281粒油松种子进行父本分析,在80%的置信水平下确定了其中126粒种子的花粉来源;对2株母树自由授粉种子的父本分析表明,种子园内花粉传播距离最大为85m,有效距离在30m以内;对11#和24#母树分别用21个无性系的等体积混合花粉进行控制授粉发现,子代由少数无性系的花粉受精产生,其中50#、7#、25#、33#无性系对2株母树子代的花粉贡献率分别达到50.62%和61.54%;花粉粒大小、花粉等体积重和花粉活力与选择性受精相关不显著,而花粉活力偏低的无性系也有授粉成功的可能性。 展开更多
关键词 油松种子园 控制授粉 自由授粉 微卫星标记 父本分析
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