A new method for the determination of antithrombotic activity of egg white protein hydrolysate （EWPH） was developed using a microplate reader. Reaction was carried out at 37℃and pH 7.2 with fibrinogen concentration...A new method for the determination of antithrombotic activity of egg white protein hydrolysate （EWPH） was developed using a microplate reader. Reaction was carried out at 37℃and pH 7.2 with fibrinogen concentration 0.1%. Microplate reading was conducted at 405 nm. Inhibition rate of EWPH on thrombin activity showed linearity （R2 = 0.9971）, when the inhibition rate was in the range of 10-90%. The lower limit of detection （LLD, at 99.7% probability） and the biological limit of detection （BLD, at 99.7% probability） of the method were 10.643 and 40 mg/mL, respectively. The repeatability standard deviation （R.S.D.） was 1.08%. The standard deviation of the method was ±0.027 AT-U.展开更多
During the Paleozoic, the Ordos area in the western North China Plate was located at the intersecting position of microplates and controlled by their interaction. The structural framework in the Ordos area, which unde...During the Paleozoic, the Ordos area in the western North China Plate was located at the intersecting position of microplates and controlled by their interaction. The structural framework in the Ordos area, which underwent transformations in the Ordovician, the Carboniferous and the Permian respectively, was dominated by the alternation of uplift and depression. The transformations of structural framework are utilized as the clues to investigate the microplates＇ interacting type and its response in the Ordos area. According to the regional structural evolution, the Ordos area is simplified into an isopachous, isotropic and elastic shell model, and under proposed various boundary conditions, three series of numerical simulations corresponding to the three structural transformations are carried out to determine the detailed tectonic constraints. Numerical simulations reveal that the structure of the uplift and depression, which is similar to the actual pattern, develops only under one special boundary condition in each of the three series, indicating that the structural framework responds to the unique tectonic background. The simulation results show that in the Early Paleozoic, the L-shaped paleouplift formed nearby the southwestern corner of the Ordos area because the intensity of the compressions in the southern and western boundaries resulting from the ocean-continent collisions was similar. In the Late Paleozoic, it evolved into continent-continent （or arc-continent） interaction in the southern and northern boundaries; in the preliminary stage of the interaction, since the interface between the North China Plate and the plates on the south and north was narrow, the relative acting force was little and the regional western boundary immobile, and the structural framework in the basin was characterized by the N-S trending slender-waist-shaped uplift; as the interface between the plates expanded gradually, the extrusive force in the southern and northern boundaries of the North China Plate increased, resulti展开更多
POD activity in heolymph of shrimps determined on 96 microplate was reported first time. In order to testify the sensitivity of using the 96 microwell plate to determine the POD activity, two methods including Worthin...POD activity in heolymph of shrimps determined on 96 microplate was reported first time. In order to testify the sensitivity of using the 96 microwell plate to determine the POD activity, two methods including Worthington and 96 microplate were used to determine the POD of Penaeus japonicus and crawfishes respectively, the results show that the date from the two methods have a strong linear correlation and prove that the method determining POD activity in hemolymph of shrimps was reliable.展开更多
A microtiter plate assay (MPA) for soluble reactive phosphorus (SRP) was applied to samples in overlying water and pore water as well as in three forms of environmental soil test phosphorus (P) types: water soluble ph...A microtiter plate assay (MPA) for soluble reactive phosphorus (SRP) was applied to samples in overlying water and pore water as well as in three forms of environmental soil test phosphorus (P) types: water soluble phosphorus (WSP),diluted calcium chloride extractable phosphorus (PCaCl2), and Olsen-P in the sediments of Taihu Lake, China, where potential P release in response to pH was analyzed. MPA for rapid P analysis was shown to be promising when applied on samples of natural water and sediment extracts. Concentrations of WSP and PCaCl2 in the sediments were much lower than those of Olsen-P. Olsen-P levels in the littoral sediments along the north coast of Meiliang Bay in Taihu Lake (80to 140 mg kg-1) were much higher than those in the mouth of the bay (less than 50 mg kg-1). The risk of P release in the mouth area of Meiliang Bay was lower than that in the north littoral zone with a risk of sediment P release induced by pH increases.展开更多
文摘A new method for the determination of antithrombotic activity of egg white protein hydrolysate （EWPH） was developed using a microplate reader. Reaction was carried out at 37℃and pH 7.2 with fibrinogen concentration 0.1%. Microplate reading was conducted at 405 nm. Inhibition rate of EWPH on thrombin activity showed linearity （R2 = 0.9971）, when the inhibition rate was in the range of 10-90%. The lower limit of detection （LLD, at 99.7% probability） and the biological limit of detection （BLD, at 99.7% probability） of the method were 10.643 and 40 mg/mL, respectively. The repeatability standard deviation （R.S.D.） was 1.08%. The standard deviation of the method was ±0.027 AT-U.
文摘During the Paleozoic, the Ordos area in the western North China Plate was located at the intersecting position of microplates and controlled by their interaction. The structural framework in the Ordos area, which underwent transformations in the Ordovician, the Carboniferous and the Permian respectively, was dominated by the alternation of uplift and depression. The transformations of structural framework are utilized as the clues to investigate the microplates＇ interacting type and its response in the Ordos area. According to the regional structural evolution, the Ordos area is simplified into an isopachous, isotropic and elastic shell model, and under proposed various boundary conditions, three series of numerical simulations corresponding to the three structural transformations are carried out to determine the detailed tectonic constraints. Numerical simulations reveal that the structure of the uplift and depression, which is similar to the actual pattern, develops only under one special boundary condition in each of the three series, indicating that the structural framework responds to the unique tectonic background. The simulation results show that in the Early Paleozoic, the L-shaped paleouplift formed nearby the southwestern corner of the Ordos area because the intensity of the compressions in the southern and western boundaries resulting from the ocean-continent collisions was similar. In the Late Paleozoic, it evolved into continent-continent （or arc-continent） interaction in the southern and northern boundaries; in the preliminary stage of the interaction, since the interface between the North China Plate and the plates on the south and north was narrow, the relative acting force was little and the regional western boundary immobile, and the structural framework in the basin was characterized by the N-S trending slender-waist-shaped uplift; as the interface between the plates expanded gradually, the extrusive force in the southern and northern boundaries of the North China Plate increased, resulti
文摘POD activity in heolymph of shrimps determined on 96 microplate was reported first time. In order to testify the sensitivity of using the 96 microwell plate to determine the POD activity, two methods including Worthington and 96 microplate were used to determine the POD of Penaeus japonicus and crawfishes respectively, the results show that the date from the two methods have a strong linear correlation and prove that the method determining POD activity in hemolymph of shrimps was reliable.
基金Project supported by the National Key Basic Research Support Foundation (NKBRSF) of China (No. 2002CB412304) the Chinese Academy of Sciences (No. KZCX1-SW-Ⅱ-32).
文摘A microtiter plate assay (MPA) for soluble reactive phosphorus (SRP) was applied to samples in overlying water and pore water as well as in three forms of environmental soil test phosphorus (P) types: water soluble phosphorus (WSP),diluted calcium chloride extractable phosphorus (PCaCl2), and Olsen-P in the sediments of Taihu Lake, China, where potential P release in response to pH was analyzed. MPA for rapid P analysis was shown to be promising when applied on samples of natural water and sediment extracts. Concentrations of WSP and PCaCl2 in the sediments were much lower than those of Olsen-P. Olsen-P levels in the littoral sediments along the north coast of Meiliang Bay in Taihu Lake (80to 140 mg kg-1) were much higher than those in the mouth of the bay (less than 50 mg kg-1). The risk of P release in the mouth area of Meiliang Bay was lower than that in the north littoral zone with a risk of sediment P release induced by pH increases.
